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A kind of fermentation production method of high-yield glutamine

A technology of glutamine and production method, which is applied in the field of high-yield glutamine fermentation production, can solve the problems of less L-glutamine, the difference in the acid production level of strains, etc., so as to improve acid production, reduce carbon frame loss, reduce cost effect

Active Publication Date: 2018-04-27
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Glutamine is mainly produced by industrial microbial fermentation. At present, there are very few enterprises in my country that have truly industrialized the production of L-glutamine, and the level of acid production by strains is far behind that of advanced foreign countries.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A fermentative production method for high-yield glutamine, comprising the following steps:

[0025] Step 1. First use Corynebacterium glutamicum YG-3131 for seed culture, and control the air volume and speed during the 7-9 hours of the culture process to reduce the dissolved oxygen to 15-20%. After the culture is completed, the strains are obtained for use;

[0026] Step 2: Inoculate the bacterial classification obtained in step 1 into the fermentation medium according to the inoculation amount of 10%. In the described fermentation medium, except water, it contains 7% glucose, 1% corn steep liquor, NH 4 Cl2%, KH 2 PO 4 0.2%, MgSO 4 0.08%, KCl0.1%, according to each liter, each liter of fermentation medium also contains yeast powder 1g / L, FeSO 4 20mg / L, CuSO 4 2mg / L and thiamine 1mg / L; Also add the inhibitor citric acid that mass percentage is 0.1% in the described fermentation medium, and the activator of glutamine synthetase, the activator of glutamine synthetase ...

Embodiment 2

[0031] A fermentative production method for high-yield glutamine, comprising the following steps:

[0032] Step 1. First use Corynebacterium glutamicum YG-3131 for seed culture, and control the air volume and speed during the 7-9 hours of the culture process to reduce the dissolved oxygen to 15-20%. After the culture is completed, the strains are obtained for use;

[0033] Step 2, inoculate the strain obtained in step 1 into the fermentation medium according to the inoculum size of 15%, in the described fermentation medium, contain glucose 3%, corn steep liquor 1%, NH 4 Cl2%, KH 2 PO 4 0.2%, MgSO 4 0.2%, KCl0.2%, according to each liter, each liter of fermentation medium also contains yeast powder 1.5g / L, FeSO 4 10mg / L, CuSO 4 10mg / L and thiamine 10mg / L; Also add the inhibitor citric acid that mass percentage is 0.2% in the described fermentation medium, and the activator of glutamine synthetase, the activator of glutamine synthetase by MnCl 2 and ZnSO 4 Composition, th...

Embodiment 3

[0038] A fermentative production method for high-yield glutamine, comprising the following steps:

[0039] Step 1. First use Corynebacterium glutamicum YG-3131 for seed culture, and control the air volume and speed during the 7-9 hours of the culture process to reduce the dissolved oxygen to 15-20%. After the culture is completed, the strains are obtained for use;

[0040] Step 2, according to the inoculation amount of 13%, inoculate the bacterial classification obtained in step 1 into the fermentation medium, in the described fermentation medium, contain glucose 5%, corn steep liquor 2%, NH 4 Cl3%, KH 2 PO 4 0.3%, MgSO 4 0.1%, KCl0.15%, according to each liter, each liter of fermentation medium also contains yeast powder 1.2g / L, FeSO 4 15mg / L, CuSO 4 6mg / L and thiamine 6mg / L; Also add the inhibitor citric acid that mass percentage is 0.15% in the described fermentation medium, and the activator of glutamine synthetase, the activator of glutamine synthetase by MnCl 2 and...

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Abstract

The invention discloses a high-yield glutamine fermentation production method, which comprises the following steps: Step 1, seed cultivation; Step 2, inoculating the strain obtained in Step 1 into a fermentation medium for cultivation; during the fermentation process, controlling the fermentation cultivation Glucose content, temperature, pH value, dissolved oxygen and other conditions of the base; step 3, after the fermentation is completed, extract and refine glutamine from the fermentation broth. The high-yield glutamine fermentation production method provided by the invention has the advantages of low raw material cost, easy control, and high fermentation acid production, with an average acid production as high as 79.8g / L.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, and in particular relates to a high-yield glutamine fermentation production method. Background technique [0002] L-Glutamine is an essential amino acid for the human body. It has special effects such as improving human immunity and significantly promoting brain function and gastrointestinal function. At present, it has a wide range of applications and good development prospects in the fields of food health care, feed additives, medicine and health. [0003] Glutamine is mainly produced by industrial microbial fermentation. At present, there are very few enterprises in my country that have truly industrialized the production of L-glutamine, and the level of acid production by strains is far behind that of advanced foreign countries. Contents of the invention [0004] The object of the present invention is to address the deficiencies in the prior art, and provide a high-yield gl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14C12R1/15
Inventor 岳贵龙孙朝阳徐晓鹏
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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