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A method for improving production efficiency of l-tryptophan

A technology for production efficiency and tryptophan, which is applied in the field of improving the production efficiency of L-tryptophan, can solve the problems of bacterial strain death and difficult operation, and achieve the effect of increasing production, acid production rate and yield

Active Publication Date: 2021-06-29
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The applicant has made in-depth research on the fermentation of L-tryptophan. For example, "CN201711289822" discloses the method of fermenting with a single strain, adding tourmaline and using dialysis medium to increase the amount of acid produced by fermentation; this method improves the fermentation Acid production, but the medium needs to be replaced, the operation is difficult, and it is easy to cause the death of some strains

Method used

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  • A method for improving production efficiency of l-tryptophan
  • A method for improving production efficiency of l-tryptophan
  • A method for improving production efficiency of l-tryptophan

Examples

Experimental program
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Embodiment 1

[0036] A method for improving L-tryptophan production efficiency, comprising the steps of:

[0037] The components of the fermentation medium are: glucose 20g / L, dipotassium hydrogen phosphate 9g / L, yeast extract 5g / L, citric acid 4.6g / L, ammonium sulfate 1.8g / L, magnesium sulfate heptahydrate 1.6g / L, Choline chloride 0.4g / L, ferrous sulfate heptahydrate 65mg / L, biotin 0.2mg / L;

[0038] The components of nutrient solution A are: 20 mg / L of cobalt chloride hexahydrate, 10 g / L of urea;

[0039]The components of nutrient solution B are: glucose 100g / L, indole 1g / L, inositol 0.5g / L, phenylalanine 0.5g / L, tyrosine 0.4g / L;

[0040] Taking the Escherichia coli strain E.coli TRTH as an example, the seed solution (OD 600 value of 11.2) into a 1000L fermenter equipped with 600L fermentation medium, the culture temperature is 36.8°C, the dissolved oxygen is 20%, and 25% ammonia water is automatically added by numerical control to maintain the system pH in the tank at 7.0-7.2. Add a de...

Embodiment 2

[0044] A method for improving L-tryptophan production efficiency, comprising the steps of:

[0045] The components of the fermentation medium are: glucose 20g / L, dipotassium hydrogen phosphate 9g / L, yeast extract 5g / L, citric acid 4.6g / L, ammonium sulfate 1.8g / L, magnesium sulfate heptahydrate 1.6g / L, Choline chloride 0.4g / L, ferrous sulfate heptahydrate 65mg / L, biotin 0.2mg / L;

[0046] The components of nutrient solution A are: 30 mg / L of cobalt chloride hexahydrate, 15 g / L of urea;

[0047] The components of nutrient solution B are: glucose 100g / L, indole 1g / L, inositol 0.5g / L, phenylalanine 0.4g / L, tyrosine 0.3g / L;

[0048] Taking the Escherichia coli strain E.coli TRTH as an example, the seed solution (OD 600 value of 11.8) into a 1000L fermenter equipped with 600L fermentation medium, the culture temperature is 36.8°C, the dissolved oxygen is 20%, and 25% ammonia water is automatically added by numerical control to maintain the system pH in the tank at 7.0-7.2. Add a d...

Embodiment 3

[0058] To verify the effects of phenylalanine and tyrosine on the yield of tryptophan and the rate of fermentation acid production, method 1: add 0.5g / L phenylalanine and 0.4g / L tyrosine to the fermentation medium; method 2 : Add 0.5g / L phenylalanine and 0.4g / L tyrosine to the nutrient solution; figure 1 As shown, mode 2 chooses to add phenylalanine and tyrosine to the nutrient solution, and the yield of tryptophan and the rate of fermentation acid production are higher than that of mode 1. The nutrient solution is added in the middle of fermentation. At this time, the aromatic amino acid The production of phenylalanine is in the process of rapid accumulation. By adding phenylalanine and tyrosine, there will be a feedback inhibition effect on the conversion of mycolic acid to prebenzoic acid, so that more mycolic acid flows to the anthranilic acid pathway, making The yield of tryptophan was increased; however, the way 1 was added to the fermentation medium, the accumulation of...

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Abstract

The invention belongs to the technical field of L-tryptophan production, and discloses a method for improving the production efficiency of L-tryptophan. The Escherichia coli seed liquid producing L-tryptophan is inserted into the In the fermenter with fermentation medium, the culture temperature is 36-37°C, the dissolved oxygen is 20-30%, and the pH of the tank system is maintained at 6.8-7.2 by automatic feeding of ammonia water through numerical control, and defoaming agent is added to defoam; The total fermentation time is 40-42h to obtain L-tryptophan fermentation broth. Based on the prior art, the present invention needs to further optimize the fermentation conditions to increase the yield of L-tryptophan.

Description

technical field [0001] The invention belongs to the technical field of L-tryptophan production, and in particular relates to a method for improving the production efficiency of L-tryptophan. Background technique [0002] The molecular formula for L-tryptophan is C 11 h 12 o 2 N 2 , with a molecular weight of 204.21 and a nitrogen content of 13.72%. L-Tryptophan is a neutral aromatic amino acid containing indole groups. It is silky luster, hexagonal flaky self-color crystal, odorless and sweet. The solubility in water is 1.14g / L (25°C). Dilute acid or alkali, relatively stable in lye, decomposed in strong acid, slightly soluble in ethanol, insoluble in chloroform and ether. [0003] L-tryptophan is one of the eight essential amino acids in the life activities of humans and animals. It plays an important role in the growth, development and metabolism of humans and animals. It is called the second essential amino acid. L-tryptophan plays an important role in the growth an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/22C12N1/20C12R1/19
CPCC12N1/20C12P13/22
Inventor 包鑫冯世红张宗华刘福玲边恩来史静东李江雷崔小红
Owner XINJIANG FUFENG BIOTECH
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