A method for producing isopropanol and butanol by fermenting lignocellulose as raw material

A technology for lignocellulose and isopropanol, which is applied in the field of fermentation production of isopropanol and butanol, can solve the problems that the product yield needs to be improved, the construction process is complicated, and the effect needs to be improved, so as to improve the fermentation effect and reduce the metabolic flux. , the effect of reducing fermentation costs

Active Publication Date: 2022-04-08
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The above-mentioned methods for producing isopropanol and butanol are basically realized by engineering bacteria constructed by genetic engineering, the construction process is complicated, and the product yield needs to be improved
In addition, a certain amount of ethanol will be generated, and the effect needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:3mL, enter them into the retainer of the steam explosion device, maintain them at 150°C and a pressure of 1.0MPa for 10 minutes, and instantly release the pressure to explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 19.11% (relative to dry matter), cellulose The content is 39.7% (relative to dry matter).

[0038] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 10%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 30FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 5.0 , Enzymolysis at 50°C, 150r / min for 72h. After testing, the concentration of xylose was 19g / L, the concentration of glucose was 39g / L, the enzymati...

Embodiment 2

[0043] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:2mL, enter them into the retainer of the steam explosion device, maintain them at 120°C and a pressure of 1.3MPa for 20 minutes, and instantly release the pressure and explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 15.86% (relative to dry matter), cellulose The content is 42.71% (relative to dry matter).

[0044] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 8%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 20FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 4.5 , Enzymolysis at 45°C, 100r / min for 72h. After testing, the concentration of xylose was 13 g / L, the concentration of glucose was 33 g / L, the enzym...

Embodiment 3

[0049] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:4mL, enter them into the retainer of the steam explosion device, maintain them at 160°C and a pressure of 0.8MPa for 5 minutes, and instantly release the pressure and explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 20.34% (relative to the dry matter), cellulose The content is 38.57% (relative to dry matter).

[0050] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 20%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 50 FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 5.5 , Enzymolysis at 55°C, 200r / min for 72h. After testing, the concentration of xylose was 45 g / L, the concentration of glucose was 73 g / L, the ...

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Abstract

The invention relates to a method for producing isopropanol and butanol by fermenting lignocellulose as a raw material. Firstly, the lignocellulose raw material is subjected to steam explosion pretreatment at 120-160°C and 0.8-1.3 MPa; and then the pretreated material is Carry out enzymatic hydrolysis to obtain cellulose and hemicellulose hydrolysis mixed sugar liquid; then use the hydrolyzed mixed sugar liquid as carbon source, add nitrogen source, nutrient salt and vitamins to prepare fermentation medium; inoculate the seed liquid of Clostridium beijerinckii XH0906 Into the fermentation medium, fermented to produce butanol and isopropanol. The invention first performs steam explosion pretreatment on lignocellulosic raw materials, and then uses Clostridium beijerinckii XH0906 to ferment and produce butanol and isopropanol, and has the characteristics of simple preparation process and high fermentation yield.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for fermenting and producing isopropanol and butanol by using lignocellulose as a raw material. Background technique [0002] Butanol is an important organic chemical raw material, which is widely used in chemical, pharmaceutical and petroleum industries. Butanol has two more methylene groups than ethanol. Compared with ethanol, butanol has higher hydrophobicity and lower volatility. It can be mixed with gasoline in any proportion, and has a calorific value equivalent to gasoline. As a potential renewable bioenergy that can replace gasoline, butanol has attracted more and more attention from all over the world. [0003] Isopropanol is an important chemical product and raw material. It is mainly used as dehydrating agent and cleaning agent in pharmaceutical, cosmetics, plastics, spices, coatings and electronic industries. Isopropanol is widely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12P7/04C12R1/145
CPCC12P7/04C12P7/16C12P2203/00C12P2201/00Y02E50/10
Inventor 王领民张全高慧鹏关浩乔凯曹长海
Owner CHINA PETROLEUM & CHEM CORP
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