A method for producing isopropanol and butanol by fermenting lignocellulose as raw material
A technology for lignocellulose and isopropanol, which is applied in the field of fermentation production of isopropanol and butanol, can solve the problems that the product yield needs to be improved, the construction process is complicated, and the effect needs to be improved, so as to improve the fermentation effect and reduce the metabolic flux. , the effect of reducing fermentation costs
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Embodiment 1
[0037] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:3mL, enter them into the retainer of the steam explosion device, maintain them at 150°C and a pressure of 1.0MPa for 10 minutes, and instantly release the pressure to explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 19.11% (relative to dry matter), cellulose The content is 39.7% (relative to dry matter).
[0038] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 10%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 30FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 5.0 , Enzymolysis at 50°C, 150r / min for 72h. After testing, the concentration of xylose was 19g / L, the concentration of glucose was 39g / L, the enzymati...
Embodiment 2
[0043] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:2mL, enter them into the retainer of the steam explosion device, maintain them at 120°C and a pressure of 1.3MPa for 20 minutes, and instantly release the pressure and explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 15.86% (relative to dry matter), cellulose The content is 42.71% (relative to dry matter).
[0044] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 8%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 20FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 4.5 , Enzymolysis at 45°C, 100r / min for 72h. After testing, the concentration of xylose was 13 g / L, the concentration of glucose was 33 g / L, the enzym...
Embodiment 3
[0049] (1) Take crushed corn stalks, soak them in tap water according to the solid-to-liquid ratio of 1g:4mL, enter them into the retainer of the steam explosion device, maintain them at 160°C and a pressure of 0.8MPa for 5 minutes, and instantly release the pressure and explode to obtain neutral Steam explosion pretreated corn stover. Mainly composed of xylose, xylooligosaccharide, xylan, cellulose and lignin, the dry matter concentration is 32%, the content of xylose, xylooligosaccharide and xylan is 20.34% (relative to the dry matter), cellulose The content is 38.57% (relative to dry matter).
[0050] (2) Add the pretreated corn stalks to tap water to adjust the dry matter concentration to 20%. The cellulase used is Novozymes Ctec2, the amount of cellulase added is 50 FPIU / g cellulose, and the pH value of enzymatic hydrolysis is 5.5 , Enzymolysis at 55°C, 200r / min for 72h. After testing, the concentration of xylose was 45 g / L, the concentration of glucose was 73 g / L, the ...
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