Method for extracting glutamine from fermentation liquor

An extraction method and glutamine technology, applied in the field of fermentation, can solve problems such as large amount of exchange resin, high acid-base consumption, environmental pollution, etc., and achieve the effects of improving yield, reducing energy consumption and high safety.

Active Publication Date: 2013-02-13
TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yin-yang double-column separation process has the problems of large amount of exchange resin and high consumption of acid and alkali in the process of elution and regeneration, resulting in serious environmental pollution.
In addition, glutamine is easily converted into glutamic acid in a strong acid or alkali environment, resulting in a very low yield

Method used

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  • Method for extracting glutamine from fermentation liquor
  • Method for extracting glutamine from fermentation liquor
  • Method for extracting glutamine from fermentation liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: The method for extracting glutamine from fermentation broth described in the present invention extracts glutamine

[0062] Take 27L of glutamine fermentation broth (glutamine content is 78mg / mL), filter with a molecular weight cut-off of 30kDa ceramic membrane to obtain the filtrate, the filtrate is concentrated in vacuum until the glutamine content is 30%, then slowly cool down to 7°C, cool and crystallize After 10 hours, centrifuge to obtain 2302.6 grams of primary crystallization crude product, add 1064 mL of water to wash the crystal, and obtain 2201.8 g of washed crystal crude product after separation, add water to dissolve until the glutamine content is 3.9%, add 856 mL of cation exchange resin to the solution and stir for 2 hours , filter, then add 1150mL of anion exchange resin, stir for 2 hours, filter to obtain 29.08L of filtrate, the filtrate is passed through a nanofiltration membrane with a molecular weight cut-off of 1000Da to obtain the nanof...

Embodiment 2

[0063]Embodiment 2: the method for extracting glutamine from fermentation broth described in the present invention extracts glutamine

[0064] Take 28L of glutamine fermentation broth (glutamine content is 76mg / mL), and filter it with a ceramic membrane with a molecular weight cut-off of 30kDa to obtain the filtrate. hours, centrifuged to obtain 2250.1 grams of primary crystallization crude product, added 1070mL water to wash the crystal, and separated to obtain 2133.3 grams of washed crystal crude product, added water to dissolve until the glutamine content was 3.7%, first added 890mL cation exchange resin to the solution, and stirred for 2 hours , filter, then add 1190mL of anion exchange resin, stir for 2 hours, filter to obtain 32L of filtrate, the filtrate is passed through a nanofiltration membrane with a molecular weight cut-off of 1000Da to obtain the nanofiltration, add 88.8 grams of injection charcoal for decolorization, filter, and the decolorization solution is conc...

Embodiment 3

[0065] Embodiment 3: Existing method for extracting glutamine extracts glutamine

[0066] Take 32L of glutamine fermentation broth (glutamine content is 75.2mg / mL), filter the filtrate with a molecular weight cut-off of 30kDa ceramic membrane, pass through an anion and cation exchange column, the volume of the cation resin is 9L, and the volume of the anion resin is 9L, and the resulting The desalted solution was concentrated in vacuo until the glutamine content was 25%, slowly cooled to 7°C, cooled and crystallized for 10 hours, and centrifuged to obtain a crude product of 1 g of crystals, which was dissolved in water until the glutamine content was 4.5%, and 120 g of charcoal for injection was added. Decolorization, filtration, the decolorization liquid obtained by filtration is passed through a nanofiltration membrane with a molecular weight cut-off of 800Da to obtain a nanofiltration liquid, concentrated to a glutamine content of 40%, the concentrated liquid is cooled to be...

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Abstract

The invention belongs to the technical field of fermentation and discloses a method for extracting glutamine from fermentation liquor. The method comprises the following steps of: cooling and filtering glutamine fermentation liquor, concentrating, cooling and crystallizing the obtained filtrate, and separating to obtain a crystallized crude product; adding 30-60 weight percent of water for washing the crystal, and separating to obtain a crude product in which the crystal is washed; and adding water for dissolving, sequentially adding a cation exchange resin and an anion exchange resin for removing salt, and filtering to obtain desalted liquid; performing nanofiltration to obtain nanofiltration liquid, decolorizing and filtering to obtain decolorized solution, concentrating, cooling, crystallizing, separating and drying to obtain glutamine. According to the method, the crude product is washed and crystallized, most of impurities on the surface of the crystallized crude product are removed, and the amount of the desalted resin is reduced, so that the sewage quantity is reduced, the energy consumption is reduced, and the pollution is reduced; and meanwhile, the method of sequentially adding the cation exchange resin and the anion exchange resin into the feed liquid is used for desalting, the glutamine loss caused by long desalting period is greatly reduced, and the yield of the glutamine is improved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a method for extracting glutamine from fermentation broth. Background technique [0002] L-glutamine (Gln) is a conditionally essential amino acid for the γ-carboxyl amidation of L-glutamic acid. It is one of the 20 basic amino acids that make up proteins. It can be converted from glucose in the body. Animal non-essential amino acids. L-glutamine is a neutral amino acid, which is easily decomposed into glutamic acid or cyclized into pyrrolidone dicarboxylic acid under partial acidity, partial alkalinity and high temperature. Its chemical molecular formula is C 5 h 10 o 3 N 2 , the molecular weight is 146.15, the decomposition temperature is 184-185°C, the isoelectric point is 5.65, and the structural formula is as follows: [0003] [0004] Studies in recent years have shown that glutamine is the most abundant amino acid in the body, accounting for more than 60% of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C237/06C07C231/24
Inventor 闫美凤尹春蕾王海雷刘康乐龚华
Owner TONGLIAO MEIHUA BIOLOGICAL SCI TECH CO LTD
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