44 results about "Norvaline" patented technology

The instant invention is drawn to the methods and compositions necessary to provide recombinant proteins with a substantially reduced or eliminated content of norleucine or other non-standard amino acids. Various embodiments of the invention provide for the substantial elimination of the incorporation of non-standard amino acids into recombinant proteins by the co-expression or enhanced expression of a protein (or the enzymatically active portion thereof) capable of degrading norleucine or other non-standard amino acids, including norvaline, beta-methylnorleucine, and homoisoleucine. In certain particular embodiments of the invention, the norleucine is degraded by a glutamate dehydrogenase, a leucine dehydrogenase, a valine dehydrogenase, a phenylalanine dehydrogenase, a glutamate/leucine/phenylalanine/valine dehydrogenase, or an opine dehydrogenase. Also provided are the cells and DNA constructs for carrying out these methods.

The invention discloses a synthesizing method of D-norvaline, which comprises the following steps: adopting n-pentatonic acid as main original material; acylating and chlorinating; bromining; ammonifying; detaching; recrystallizing; hydrolyzing.

The invention discloses a synthesizing method of chiral norvaline, which comprises the following steps: adopting n-butyladehyde, sodium cyanide and ammonia chloride as raw material; proceeding cyano amidation; detaching; recrystallizing; hydrolyzing.

The invention discloses a synthesizing method of L-norvaline based on valerianic acid as raw material, which comprises the following steps: 1) proceeding acylated chloride reaction for valerianic acid acted by sulfone chloride to obtain valeryl chloride; 2) reacting valeryl chloride and liquid bronmine; removing sulfone chloride and bromine to obtain the rough product of alpha-bromovaleryl chloride; 3) dissolving alpha-bromovaleryl chloride in the solvent to ammonolyze; washing; condensing to obtain racemic alpha-aminovaleramide; 4) detaching the racemic alpha-aminovaleramide to obtain L-aminovaleramide tartrate; 5) recrystallizing; 6) hydrolyzing.

A composition of variant biocatalysts, specifically variants of D-amino acid oxidases, with improved biocatalytic activity towards D-amino acid substrates such as, but not limited to, D-tert-leucine, D-norvaline, D-2-aminobutyrate, D-alanine, D-isoleucine, D-valine, D-methionine, D-hydroxyadamantlyglycine, D-penicillamine, or D-norleucine is disclosed. Further disclosed is a method of preparing enantioselective amino acids using variant D-amino acid oxidases of the present invention.

The invention discloses a preparation method of a tobacco extracting solution that is used for amino acid analysis, takes HCl solution that contains norvaline as extracting solution, extracts tobacco ashes under ultrasonic oscillation, and obtains the extracting solution by filtering. The extracting solution is combined with a coupling technique of a liquid chromatogram and an electrospray tandom mass spectrometry, thus providing a complete experimental proposal which avoids fuzzy pre-column and post-column derivatization operations of tobacco samples, obtains good chromatogram peak form and proper chromatogram running time in the experiment, further leads the isomerides Leu and Ile to reach baseline separation, and consequently obtains accurate analyzing result.

Prodrugs of glutamine analogs, such as prodrugs of aza-serine, and 6-diazo-5-oxo-norleucine (DON), and 5-diazo-4-oxo-L-norvaline (L-DONV) are disclosed.

The invention discloses a method for inducing rice to improve salt tolerance, and belongs to the technical field of rice planting. The method comprises the following steps of 1, disinfecting the surfaces of rice seeds, and then dissolving 5-hydroxy-L-norvaline with a 0.3-1 wt.% NaCl solution to prepare a 5-hydroxy-L-norvaline solution with the concentration of 20-400 mg/L; and 2, soaking the riceseeds in the 5-hydroxy-L-norvaline solution prepared in the step 1 at the temperature of 20-28 DEG C for 16-24 hours to obtain treated rice seeds, and enabling the treated rice seeds to normally germinate and grow in a salt stress environment. According to the method, the exogenous 5-hydroxy-L-norvaline is used for soaking the rice seeds and irrigating, so that the germination rate and the growthrate of the induced rice seeds can be improved under the salt stress condition, and the rice can be effectively induced to improve the salt tolerance; and the method can meet the urgent requirements of coastal beaches in China for high-yield, high-quality, disease-resistant, salt-resistant and direct-seeding rice varieties, and has very high application potential and value.

The invention provides a method for determining other amino acids in an L-valine raw material by high performance liquid chromatography. The method comprises the following steps of preparing L-valine,leucine, isoleucine, glycine, alanine and phenylalanine reference substance solutions; detecting through a high performance liquid chromatograph, comparing the appearance time and peak shape of L-valine, leucine, isoleucine, glycine, alanine and phenylalanine in a chromatogram of the test sample with those of L-valine, leucine, isoleucine, glycine, alanine and phenylalanine in a chromatogram of the reference substance solution, and judging whether the test sample solution contains other amino acids or not. According to the method, by screening an aminopropyl bonded silica gel chromatographiccolumn, the separation effects are found, and by screening the wavelengths, the maximum absorption of each amino acid is found, and the retention time of each amino acid peak on the chromatographic column is regulated and controlled by regulating the ratio of triethylamine, the pH value and the ratio of two phases by utilizing an acetonitrile/phosphate buffer solution binary mobile phase, so thatthe hydrolysis and shedding of bonded aminopropyl in the amino column are effectively prevented, and the problems of short reverse-phase service life and unstable baseline of the amino column are solved.

The invention provides a method for synthesizing L-norvaline. The method comprises the following steps: 1, adding acetic acid into a reaction kettle, adding DL-norvaline and D-tartaric acid while stirring, heating to 80DEG C, adding salicylaldehyde, and maintaining the temperature in a range of 80-85DEG C for 10h; 2, cooling to 20-25DEG C after temperature maintenance, keeping the decreased temperature for 1h, and centrifuging to obtain crude L-norvaline and D-tartaric acid; 3, adding petroleum ether to the crude L-norvaline and D-tartaric acid, stirring and washing for 30min, and centrifuging to obtain a purified L-norvaline and D-tartaric acid double salt; 4, heating the double salt in water to 70DEG C in order to completely dissolve the double salt, adjusting the pH value to 7 by using ammonia water, cooling to 20-25DEG C, crystallizing for 2h, centrifuging, and rinsing by using methanol to obtain crude L-norvaline; and 5, heating the crude L-norvaline in water to 80-85DEG C in order to completely dissolve the crude L-norvaline, adding active carbon, decoloring for 1h, filtering, concentrating, cooling to 20-25DEG C, crystallizing for 3h, centrifuging, rinsing with water to obtain wet L-norvaline, and carrying out 60DEG C vacuum drying to obtain dry L-norvaline. The method has the advantages of few steps, good environmental protection property and high yield.

The invention relates to daily cosmetics, and particularly relates to physical sunscreen cream. The cream is prepared from water-in-oil emulsion, coconut oil, octyl methoxycinnamate, glycerinum, hyaluronic acid, titanium dioxide, vitamin A palmitate, peppermint essential oil, lemon oil, lycopene, norvaline, arginine, hydroxyproline, cysteine and deionized water. The physical sunscreen cream does not contain harmful chemical components or heavy metal components, does not irritate skin, is long in duration, contains multiple amino acids, is easy to clean, and has a good moistening effect.

The invention discloses a waterproof sunscreen, which comprises the following components: 1-5 parts of zinc oxide, 0.2-2 parts of citric acid, 0.1-1 parts of sodium hydroxymethyl glycinate, 0.5-0.8 parts of octyl cyanodiphenyl acrylate, Butylmethoxydibenzoylmethane 0.5-2 parts, polyglycerol 1-8 parts, arginine 1-3 parts, cysteine ​​0.1-0.5 parts, norvaline 0.1-0.5 parts. The waterproof sunscreen disclosed in the invention is not easily soluble in water, and has better effects of sunburn and tanning.

Norvaline and/or other amino acids that are capable of being acylated onto tRNA^Leu by LeuRS, in combination with substituted benzoxaboroles, such as a compound having a structure according to formula III: and methods for decreasing the frequency of resistance and/or reducing the rate of resistance and/or suppressing the emergence of resistance that develops in bacteria exposed to a substituted benzoxaborole or salt thereof by administering a combination of a substituted benzoxaborole such as a compound of formula III or salt thereof and an amino acid or a salt thereof.

The invention relates to the technical field of chemical synthesis, and discloses an L-norvaline preparation method, which comprises: 1) acyl chlorination: washing a reaction kettle, drying, adding thionyl chloride, starting stirring, adding n-valeric acid, and controlling the reaction temperature at 20-80 DEG C and the reaction time at 2-8 h to obtain n-valeryl chloride. According to the invention, thionyl chloride is fed, the thionyl chloride is decomposed into sulfur dioxide and hydrogen chloride or chlorinated hydrocarbon when meeting water or alcohol so as to provide the selective substitution effect on hydroxyl, the thionyl chloride is soluble in benzene, chloroform and carbon tetrachloride, is decomposed after being heated to 150 DEG C, and is completely decomposed at a temperatureof 500 DEG C to obtain n-valeryl chloride, liquid bromine reacts with the n-valeryl chloride, and has strong affinity with hydrogen, and the mixing is performed with organic matters to obtain racemicalpha-aminopentanamide, such that the yield is far higher than the yield of the amino acid produced by common fermentation, and the industrial production requirements can be met.