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Preparation method of tobacco raffinate used for amino acid analysis

A technology of tobacco extract and amino acid, which is applied in the field of chemical analysis and detection, can solve the problems of loss of amino acid and amino acid derivatives, and achieve the effects of good chromatographic peak shape, accurate analysis results, and suitable chromatographic running time

Active Publication Date: 2009-01-14
CHINA TOBACCO GUANGDONG IND
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AI Technical Summary

Problems solved by technology

Existing pre-treatments such as solid phase extraction will cause the loss of certain amino acids (or amino acid derivatives) to a certain extent, and some interfering impurities are difficult to eliminate through pre-treatment

Method used

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  • Preparation method of tobacco raffinate used for amino acid analysis
  • Preparation method of tobacco raffinate used for amino acid analysis
  • Preparation method of tobacco raffinate used for amino acid analysis

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Experimental program
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Embodiment 1

[0027] 1. Instruments, materials and reagents

[0028] LC-MS LTQ Liquid Chromatography-Ion Trap Mass Spectrometer from Thermo Electric Company, equipped with autosampler, quaternary gradient mass spectrometer pump, LTQ ion trap mass spectrometer charged spray ionization source (ESI), Xcalibur 1.4 SR1 system control software; Italy Claind N2LCMS Nitrogen generator; French Millipore Element A10 pure water machine; American Cole-Parmer 8892 ultrasonic cleaner; Sartorious CP225D electronic balance (Max 220g, d=0.01mg (80g), 0.1mg (220g)); Whatman 0.2μm nylon needle type filter head; tobacco powder from a mill.

[0029] Mixed amino acid standard solution (Fluka Company), containing 17 kinds of amino acids, the concentration is 1mM each; internal standard L-norvaline (Shanghai Livzon Dongfeng Biotechnology Co., Ltd.); asparagine water (Shanghai Boao Biotechnology Co., Ltd. Co., Ltd.); tryptophan, proline and glutamine (Sinopharm Chemical Reagent Co., Ltd.); γ-aminobutyric acid (Sig...

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Abstract

The invention discloses a preparation method of a tobacco extracting solution that is used for amino acid analysis, takes HCl solution that contains norvaline as extracting solution, extracts tobacco ashes under ultrasonic oscillation, and obtains the extracting solution by filtering. The extracting solution is combined with a coupling technique of a liquid chromatogram and an electrospray tandom mass spectrometry, thus providing a complete experimental proposal which avoids fuzzy pre-column and post-column derivatization operations of tobacco samples, obtains good chromatogram peak form and proper chromatogram running time in the experiment, further leads the isomerides Leu and Ile to reach baseline separation, and consequently obtains accurate analyzing result.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a method for preparing tobacco extract for amino acid analysis. Background technique [0002] Amino acids are an important part of tobacco leaves, and are tobacco components closely related to smoke aroma, cigarette quality and brand characteristics. Amino acids participate in the non-enzymatic brown reaction in tobacco leaves, namely the Maillard reaction, which can produce a variety of important Aroma ingredients. Therefore, the determination of amino acids in tobacco is of great significance to formula development, process production and quality control. [0003] Because there are many kinds of amino acids in tobacco, and they have three different chemical properties: acidic, neutral and basic, and the matrix of tobacco is complex, it is difficult to simultaneously measure multiple amino acids in tobacco. Currently, liquid chromatography (LC) method i...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 黄翼飞
Owner CHINA TOBACCO GUANGDONG IND
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