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Method for inducing rice to improve salt tolerance

A technology of salt tolerance and rice, applied in the field of inducing rice to improve salt tolerance, can solve the problems of slow growth, poor quality, low seedling emergence rate, etc. Effect

Active Publication Date: 2020-12-04
JIANGSU COASTAL AREA AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This application provides a good way to solve the problems of few salt-tolerant rice varieties in coastal beaches, low yield of existing salt-tolerant varieties, poor disease resistance, especially rice blast resistance, and poor quality
However, this method is not in a salt stress environment throughout the whole process, and has little effect on improving the germination rate, and it is difficult to solve the problems of low emergence rate and slow growth in salt stress environments (such as direct-seeding rice on coastal beaches)

Method used

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  • Method for inducing rice to improve salt tolerance
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Examples

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Effect test

Embodiment 1

[0022] A method for inducing rice to improve salt tolerance, the steps are as follows:

[0023] Step 1. Disinfect the surface of rice seeds, and then dissolve 5-hydroxynorvaline with 3 wt.‰NaCl solution to prepare a 5-hydroxynorvaline solution with a concentration of 20 mg / L. The specific process of surface disinfection of rice seeds As follows: select mature rice seeds with plump grains and no mold spots, rinse twice with 70 wt.% ethanol solution for 30 s each time, rinse twice with sterile water, and then sterilize with 5 wt.% NaClO solution for 5 min , rinsed with sterile water for 3 times, and finally blotted the water attached to the surface of the seeds with sterile filter paper;

[0024] Step 2. Soak the rice seeds with the 5-hydroxynorvaline solution prepared in step 1 for 16 hours at 20° C. to obtain treated rice seeds, and allow them to germinate and grow normally under salt stress. The salt stress environment is a mixed solution of 5-hydroxyl-L-norvaline and NaCl, ...

Embodiment 2

[0027] A method for inducing rice to improve salt tolerance, the steps are as follows:

[0028] Step 1. Disinfect the surface of rice seeds, and then dissolve 5-hydroxynorvaline with 10 wt.‰NaCl solution to prepare a 5-hydroxynorvaline solution with a concentration of 400 mg / L. The specific process of surface disinfection of rice seeds As follows: select mature rice seeds with plump grains and no mold spots, rinse them with 75 wt.% ethanol solution for 3 times, each time for 40 s, rinse with sterile water for 3 times, and then sterilize with 10 wt.% NaClO solution for 15 min , rinsed with sterile water for 5 times, and finally blotted the water attached to the seed surface with sterile filter paper;

[0029]Step 2. Soak the rice seeds with the 5-hydroxynorvaline solution prepared in step 1 for 24 hours at 28° C. to obtain treated rice seeds, and allow them to germinate and grow normally under salt stress. The salt stress environment is a mixed solution of 5-hydroxyl-L-norvali...

Embodiment 3

[0032] A method for inducing rice to improve salt tolerance, the steps are as follows:

[0033] Step 1. Disinfect the surface of rice seeds. The specific process of surface disinfection of rice seeds is as follows: select mature rice seeds with full grains and no mold spots, rinse them with 70 wt.% ethanol solution twice, each time for 30 s, and rinse with sterile water 2 times, then sterilized with 5wt.% NaClO solution (plus 1-2 drops of Tween 20) for 5 min, rinsed with sterile water for 3-5 times, and finally blotted the water attached to the seed surface with sterile filter paper, and then used 5 wt. .‰NaCl solution dissolves 5-hydroxyl-L-norvaline to prepare a 5-hydroxyl-L-norvaline solution with a concentration of 200 mg / L;

[0034] Step 2. Soak the rice seeds with the 5-hydroxy-L-norvaline solution prepared in step 1 for 24 hours at 25° C. to obtain treated rice seeds, and allow them to germinate and grow normally under salt stress. The salt stress environment is irriga...

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Abstract

The invention discloses a method for inducing rice to improve salt tolerance, and belongs to the technical field of rice planting. The method comprises the following steps of 1, disinfecting the surfaces of rice seeds, and then dissolving 5-hydroxy-L-norvaline with a 0.3-1 wt.% NaCl solution to prepare a 5-hydroxy-L-norvaline solution with the concentration of 20-400 mg / L; and 2, soaking the riceseeds in the 5-hydroxy-L-norvaline solution prepared in the step 1 at the temperature of 20-28 DEG C for 16-24 hours to obtain treated rice seeds, and enabling the treated rice seeds to normally germinate and grow in a salt stress environment. According to the method, the exogenous 5-hydroxy-L-norvaline is used for soaking the rice seeds and irrigating, so that the germination rate and the growthrate of the induced rice seeds can be improved under the salt stress condition, and the rice can be effectively induced to improve the salt tolerance; and the method can meet the urgent requirements of coastal beaches in China for high-yield, high-quality, disease-resistant, salt-resistant and direct-seeding rice varieties, and has very high application potential and value.

Description

technical field [0001] The invention relates to the technical field of rice planting, in particular to a method for inducing rice to improve salt tolerance. Background technique [0002] Soil salinization is the second most serious abiotic stress that restricts crop growth and development and leads to yield reduction. According to statistics, the area of ​​saline soil in the world is about one billion hectares, and the area of ​​saline soil in China reaches 100 million hectares. Due to improper irrigation and fertilization in my country's existing cultivated land, at least 8 million hectares of soil have accumulated salt, which affects the growth of crops to varying degrees. Under the severe pressure of increasing population, decreasing arable land area and serious shortage of fresh water resources, actively using large areas of salinized land has become a major issue of global concern. [0003] Rice is a moderately salt-sensitive crop. As the staple food of more than ha...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01C1/08A01H1/06A01G31/00A01N37/44A01P21/00
CPCA01C1/00A01C1/08A01H1/06A01G31/00A01N37/44A01N37/46A01P21/00A01G7/00Y02A40/135A01H3/04
Inventor 张桂云孙明法朱国永唐红生王爱民严国红刘凯施伟孙一标赵绍路宛柏杰朱静雯代金英胡蕾
Owner JIANGSU COASTAL AREA AGRI SCI RES INST
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