127 results about "Pyridoxine phosphate" patented technology

The invention discloses a microwave-assistant preparation method of hydroxylapatite nanometer structure porous microspheres. The microwave-assistant preparation method comprises the following steps of: mixing water-soluble calcium salt as a calcium source with pyridoxal phosphate as a phosphor source under an acidic condition; preparing hydroxylapatite nanometer structure porous microspheres as a carrier through a microwave-assistant hydrothermal reaction; adding the hydroxylapatite nanometer structure porous microspheres to organic solvent containing a hydrophobic drug or an aqueous solution containing hydrophilic protein; and oscillating the solution for 24-60 hours at 20-40 DEG C, and centrifuging, washing and drying the products to obtain the hydroxylapatite nanometer structure porous microspheres which are loaded with the hydrophobic drug or adsorbed with the hydrophilic protein.

The invention relates to the technical field of microorganisms, in particular to genetically engineered bacteria for increasing synthesis of PLP (pyridoxal phosphate) in escherichia coli mycetocyte and a method for producing 1,5-pentanediamine from the genetically engineered bacteria. A gene engineering technique is utilized, a key gene for synthesis of PLP through ribose-5-phosphate is subjected to heterologous expression in escherichia coli, and an efficient conversion production strain of 1,5-pentanediamine is established. Under the condition that PLP is not externally added, the genetically engineered bacteria can be converted within 4 h, and the content of produced 1,5-pentanediamine reaches 250 g / l and is far higher than that reported in the prior art.

The invention relates to the technical field of enzyme catalysis, and specifically relates to a transaminase catalyst; and the invention further relates to a method for synthesizing (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting an enzymatic way, as well as a production method of the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way comprises a step of, in the presence of pyridoxal phosphate and the transaminase catalyst, allowing reaction of N-tert-butoxycarbonyl-3-piperidinone as a reaction substrate with an amino donor so as to produce the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way utilizes relatively few reagents, and is mild in reaction conditions, so that tedious steps needed for chemical process synthesis are greatly simplified; and moreover, a target product withan ee value up to 99.77% or above can be obtained without performance of separation. Therefore, the transaminase catalyst and the process method utilizing the transaminase catalyst to synthesize the (R)-1-tert-butoxycarbonyl-3-aminopiperidine provided by the invention have broad application prospects as well as great market values.

The invention provides a biological preparation method of gamma-aminobutyric acid, which comprises the following steps of: 1) preparing glutamic acid decarboxylase whole cell thallus by using an E. coli engineering strain capable of expressing glutamic acid decarboxylase; 2) using monosodium glutamate and concentrated hydrochloric acid to prepare L-glutamic acid; 3) dissolving L-glutamic acid as asubstrate in water, adding glutamic acid decarboxylase whole cell thallus and pyridoxal phosphate, stirring and reacting to obtain gamma-aminobutyric acid. The method uses water to replace buffer salt solution to form a water-phase reaction system, uses monosodium glutamate to replace high-purity glutamic acid, so that the purity of the gamma-aminobutyric acid is up to 97.7 percent, and the costof raw materials is reduced; whole cells used for catalysis can be produced in a large scale by microbial fermentation, the cost is low, and the source is wide; the biological enzyme-promoted catalytic reaction is a water-phase reaction, the enzymatic conversion condition is mild, the raw material conversion is thorough, the post-treatment is simple, the product is separated by adopting a method of evaporation concentration and natural crystallization, the cost is low, the process is environment-friendly, and the method is suitable for industrial production of gamma-aminobutyric acid.

The object of the present invention is to provide organisms capable of producing ergothioneine which are simpler and less time-consuming than the prior art and which are capable of producing ergothioneine in high yields, thus enabling the production of ergothioneine on an industrial scale Thioneine. The above objects are solved by a transformed filamentous bacterium that has inserted a gene encoding the following enzyme (1) or genes encoding the following enzymes (1) and (2) and overexpressed the inserted gene. (1) In the presence of S-adenosylmethionine, iron (II) and oxygen, catalyze the formation of histidine trimethyl cysteine ​​sulfoxide from histidine and cysteine The enzyme of reaction; (2) with 5 ' - pyridoxal phosphate as coenzyme, catalyzes the enzyme of the reaction that generates ergothioneine by histidine trimethyl cysteine ​​sulfoxide.

The invention relates to a method for synthesizing a key enzyme cofactor by adding daptomycin to improve the daptomycin yield, which comprises the following steps of: putting streptomyces reseosporus slant spores into sterile water to prepare a spore suspension at room temperature; then inoculating the 0.5-ml spore suspension into a 250ml shake flask filled with a 30ml seed culture medium at the temperature of 28-32 DEG C, and cultivating in a 180-220rpm shaking bed for 48 hours; then adding the mixture with a 1-percent inoculated dose into an automatic 7.5LDE fermentation tank filled with a fermentation culture medium; adding one or the composition of 0.5-30mg / l cofactor heme or calcium leucovorin or pyridoxal phosphate VB6; controlling the ventilation volume to be 0.8-1.2v / v / m at the temperature of 28-32 DEG C, and fermenting for 90-144 hours with a pH value of 6.5-7.5. The method is utilized for regulating biological enzyme activity, so that an intracellular metabolic flux leading to daptomycin synthesis is increased, and the yield of the daptomycin is improved; the method has simple operation and low cost, and the fermentation unit of the daptomycin in the fermented cultivated streptomyces reseosporus can be improved by 0.24-4.6 times than a reference substance.