127 results about "Pyridoxine phosphate" patented technology

The invention discloses aminotransferase, a mutant and application to Sitagliptin preparation. According to the application, wet thalli obtained by performing fermentation culture on recombinant escherichia coli containing aminotransferase encoding genes are used as biocatalysts; Sitagliptin precursor ketone is used as a substrate; dimethyl sulfoxide is used as a latent solvent; phosphopyridoxal is used as a coenzyme; isopropylamine is used as an auxiliary substrate; a trolamine buffer solution with the pH being 8 to 9 is used as a reaction medium; a reaction system is formed; the biocatalytic reaction is performed under the conditions of the temperature being 30 to 45 DEG C and the stirring speed being 100 to 250 r / min; after the reaction is completed, the reaction liquid is separated and purified; the Sitagliptin is obtained. The aminotransferase and the mutant are used as biocatalysts; the latent carbonyl compound of Sitagliptin precursor ketone is directly used as the substrate; meanwhile, biocatalytic reaction is performed by using isopropylamine as the auxiliary substrate and using the pyridoxal phosphate as the coenzyme; the separation and purification is performed; Sitagliptin with high optical purity is prepared. The method has the advantages that the total yield is 76 percent; the product e.e. value reaches 99 percent.

The invention discloses a microwave-assistant preparation method of hydroxylapatite nanometer structure porous microspheres. The microwave-assistant preparation method comprises the following steps of: mixing water-soluble calcium salt as a calcium source with pyridoxal phosphate as a phosphor source under an acidic condition; preparing hydroxylapatite nanometer structure porous microspheres as a carrier through a microwave-assistant hydrothermal reaction; adding the hydroxylapatite nanometer structure porous microspheres to organic solvent containing a hydrophobic drug or an aqueous solution containing hydrophilic protein; and oscillating the solution for 24-60 hours at 20-40 DEG C, and centrifuging, washing and drying the products to obtain the hydroxylapatite nanometer structure porous microspheres which are loaded with the hydrophobic drug or adsorbed with the hydrophilic protein.

The invention discloses threonine aldolase, a mutant, encoding genes of the threonine aldolase and the mutant, a recombinant vector constructed by the encoding genes, recombinant genetic engineering strains obtained by transforming the recombinant vector and application of the threonine aldolase and the mutant in preparation of 2- / 3- / 4-substituted phenylserine derivatives. The threonine aldolase and the mutant serve as biocatalysts, and 2- / 3- / 4-substituted benzaldehyde serves as a substrate, 5-pyridoxal phosphate serves as coenzyme, glycine / glycine ester serves as an auxiliary substrate, and enzymic catalytic reaction is performed in a medium under appropriate conditions to separate and prepare a series of phenylserine derivatives with different substituents. According to the method, the total yield ranges from 76% to 99%, and the ee value of the product is greater than 99%, and the de value ranges from 70% to 99%.

The invention provides a method for detecting water-soluble vitamins in blood. The method includes the steps of detecting a mixed standard solution with at least three concentrations through a liquidchromatograph-mass spectrometer to obtain a first detecting result, wherein the mixed standard solution comprises internal standard substances and the water-soluble vitamins, and the water-soluble vitamins include the vitamin B1, the vitamin B2, the vitamin B3, the vitamin B5, pyridoxic acid, pyridoxal phosphate, the vitamin B7, the vitamin B9, 5-methyltetrahydrofolate, the vitamin B12 and the vitamin C; conducting fitting to obtain standard curve equations corresponding to the water-soluble vitamins on the basis of the first detecting result and the concentrations of the water-soluble vitamins; centrifuging a to-be-detected blood sample with a precipitate protein agent and a certain amount of internal standard substances, and taking supernate to be detected through the liquid chromatograph-mass spectrometer to obtain a second detecting result; obtaining the concentration of the water-soluble vitamins in the to-be-detected blood sample on the basis of the standard curve equations and the second detecting result. By means of the scheme, the detecting efficiency of the blood sample can be improved.

The invention provides a biological preparation method of R-3-aminobutanol. The method includes: conducting whole-gene synthesis according to an artificially designed sequence shown as SEQ ID NO:1, and performing cloning into pET24a so as to obtain a recombinant expression vector; transferring the recombinant expression vector into E.coli to obtain a recombinant D-transaminase genetic engineering bacterium; culturing the bacterium to prepare recombinant D-transaminase; adding 3-carbonyl butanol with a final concentration of 10-300mmol / L, dimethyl sulfoxide or acetonitrile with a mass percentage concentration of 2-15%, pyridoxal phosphate with a final concentration of 0.1-1mmol / L, isopropylamine or D-alanine with a final concentration of 0.02-1mol / L, and recombinant D-transaminase with a mass percentage concentration of 0.01-1% are added into the reaction solution to compose a reaction system for reaction; and at the end of the reaction, extracting R-3-aminobutanol from the reaction solution. With a low cost, the method provided by the invention can achieve a conversion rate of more than 97% and a product yield of greater than 87%, and does not generate by-product, thus being more suitable for industrial application.

The invention relates to the technical field of microorganisms, in particular to genetically engineered bacteria for increasing synthesis of PLP (pyridoxal phosphate) in escherichia coli mycetocyte and a method for producing 1,5-pentanediamine from the genetically engineered bacteria. A gene engineering technique is utilized, a key gene for synthesis of PLP through ribose-5-phosphate is subjected to heterologous expression in escherichia coli, and an efficient conversion production strain of 1,5-pentanediamine is established. Under the condition that PLP is not externally added, the genetically engineered bacteria can be converted within 4 h, and the content of produced 1,5-pentanediamine reaches 250 g / l and is far higher than that reported in the prior art.

The invention relates to threonine aldolase, a coding gene thereof, and an application of the threonine aldolase to biosynthesis of L-threo-DOPS, and belongs to the field of a biological technology. The threonine aldolase is protein as shown in SEQID NO.1, or protein having the same functions, obtained through replacing and / or deleting and / or adding one or more amino acid residues to an amino acidsequence as shown in SEQID NO.1. The threonine aldolase can use benzaldehyde with replaced 3- / 4- hydroxyl as a substrate, 5-pyridoxal phosphate as a coenzyme and glycine as an assistance substrate. Under the appropriate condition and in an appropriate medium, an enzyme catalysis reaction is performed, and biosynthesis is performed to obtain the L-threo-DOPS (L-threo-DOPS). The non-antipode selectivity of the method can achieve 30%. The threonine aldolase having high selectivity has important significance for biocatalysis of the L-threo-DOPS.

The invention relates to a batch type feed-batch fermentation method for streptomyces roseosporus to produce Daptomycin efficiently, comprising the following steps: taking streptomyces roseosporus slant spore at normal temperature to prepare spore suspension by sterile water; inoculating 0.5ml of the spore suspension in a 250ml shake flask filled with 30ml of seed culture medium, and culturing for 48h in a shaker when the temperature is 28-32 DEG C and the revolution speed is 180-220 rpm; adding 1% of inoculum size in an automatic 7.5 LDE fermentation tank loaded with fermentation medium; adding one or combination of cofactor ferroheme or leucovorin or pyridoxal phosphate VB6 with the concentration of 0.5-30mg / L; and controlling the throughput to be 0.8-1.2v / v / m, the pH value to be 6.5-7.5 and fermenting for 90-144h at the temperature of 28-32 DEG C. By adopting the method in the invention to regulate biological enzyme activity to increase intracellular metabolism flux introduced in Daptomycin synthesis, the Daptomycin yield is improved; the method has simple operation and low cost; and in the streptomyces roseosporus fermented and cultured by the invention, the fermentation unit of the Daptomycin can be improved by 0.24-4.6 times compared with a contrast sample.

The invention discloses a method for preparing flame retardant aqueous polyurethane coating and adhesive. Polytetrahydrofuran ether glycol and isocyanate are mixed in a 500ml four-opening flask where a stirrer, a thermometer and a condenser pipe are arranged, reaction is performed for 2.5 hours at the temperature of 75 DEG C under the condition of existing of catalyzer dibutyl tin dilaurate to obtain a polyurethane prepolymer A, pyridoxal phosphate and inrumescent flame retardants are added into the polyurethane prepolymer A, reaction is performed for 2.5 hours at the temperature of 85 DEG C, triethylamine is added for neutralization reaction for 90 minutes, water is added for emulsification, ethylenediamine tetramethylenephosphonic acid, dipotassium ethylene diamine tetraacetate and bis(4-carboxyphenyl)phenyl-phosphine oxide are added, reaction temperature is 85 DEG C, the reaction time is one hour, and a system potential of hydrogen (pH) is adjusted to 7-8 to form the flame retardant aqueous polyurethane coating and adhesive. The polyurethane material is high in flame retardant property, environmental friendly, low in price and capable of meeting low cost requirements of market.

The invention discloses an L-threonine aldolase mutant, a gene and a method for preparing L-syn-p-methylsulfonylphenylserine. The L-threonine aldolase mutant is obtained by mutation of wild type L-threonine aldolase; and with glycine and p-methylsulfonylbenzaldehyde as substrates and pyridoxal phosphate as a coenzyme, the L-syn-p-methylsulfonylphenylserine can be generated from the L-threonine aldolase mutant through a catalytic condensation reaction. The L-syn-p-methylsulfonylphenylserine produced by using the L-threonine aldolase mutant disclosed by the invention has the following advantages: 1, the production process is simple, and the reaction conditions are mild; 2, the L-threonine aldolase has high selectivity and high optical purity and does not need to be split; 3, the atom utilization rate is high and theoretically can reach 100%; 4, the production process is environment-friendly and less in pollution, and conforms to the concept of green chemistry; and 5, product separation and purification are simple.

The invention discloses an aminopherase mutant and an application thereof in the production of L-glufosinate-ammonium. The application of the aminopherase mutant in the production of L-glufosinate-ammonium is as follows: a reaction system is formed by using a wet thallus obtained through the fermentation culture of recombinant escherichia coli containing a aminopherase mutant coding gene as a biocatalyst, 2-carbonyl-4 (methyl hydroxyl phosphoryl)-butyrate as a substrate, pyridoxal phosphate as a coenzyme, an amino donor as a cosubstrate, and a buffer solution with the pH value of 6-9 as a reaction medium, and is subjected to biocatalytic reaction at the temperature of 40 to 50 DEG C and the stirring speed of 150 to 250r / min to obtain the L-glufosinate-ammonium. According to the method, thetotal yield is 98%, and the e. e. value of the product reaches 99%.

The invention relates to an enterococcus faecalis with the collection number of CGMCC No.3164. A bacterial colony is cultured on an MRS agar culture medium at the temperature of 37 DEG C for 24 hours to be gathered; the bacterial colony is white, thallus is ovate, extends along chain direction, is in pair or in short chain, is Gram-negative, generates lactic acid when fermenting glucose and is elliptic under a common microscope. When being applied, the bacterial colony is inoculated into a test tube filled with culture medium A to stand at the temperature of 37 DEG C to be cultured for 24 hours; after being repeatedly activated five times, the bacteria solution is inoculated into a culture medium B to stand at the temperature of 37 DEG C to be cultured for four days; the cultivated bacteria solution is decentralized at 1000 rpm for 10 minutes; and supernate is extracted to obtain high concentration tyramine solution. The culture medium A is an MRS liquid culture medium of 0.1% of tyrosine, and the culture medium B is an MRS liquid culture medium of 0.005% of pyridoxal phosphate and 0.1% of tyrosine.

The invention provides an L-tyrosine preparation method through enzymatic conversion. The method comprises the following steps: 1, respectively culturing strains having the activities of serine deaminase and strains having the activities of tyrosine phenol-lyase in a culture medium; 2, mixing two cells of the serine deaminase and the tyrosine phenol-lyase with an L-serine-containing amino acid material liquid, adding an aqueous phenol solution having a concentration of 500g / L, pyridoxal phosphate having a concentration of 0.2g / L and a surfactant having a concentration of 0.01-1.0g / L, adjusting the pH value to 6-11 by using ammonia water having a volume percentage of 25%, and carrying out an enzymatic reaction for 6-35h; and 3, centrifuging the obtained conversion liquid for 15min in 4000r / min, collecting the obtained mixture, dissolving the mixture by using 200-7000ml of pure water, adding 10mol / L sodium hydroxide in a dropwise manner to adjust the pH value to 12-13, heating to 80DEG C while stirring, adjusting the pH value of the obtained filtrate to 6 by using 6mol / L hydrochloric acid, and drying to obtain required L-tyrosine.

The invention discloses a genetically engineered bacterium, and applications thereof in preparation of L-phosphinothricin. The genetically engineered bacterium comprises Escherichia coli and recombinant plasmids transferred into Escherichia coli; the recombinant plasmids contain transaminase gene and pyridoxal phosphate synthetic route enzyme gene; the nucleotide sequence of the transaminase geneis represented by SEQ ID NO.1; the nucleotide sequence of the pyridoxal phosphate synthetic route enzyme gene is represented by SEQ ID NO.2-5. According to the applications, genetic engineering technology is adopted to express ribose-5-phosphate pathway synthesis key gene PdxST in Escherichia coli, or enhance Escherichia coli self PLP synthesis pathway key gene epd, pdxJ, and dxs expression; preparation of the coenzyme reinforced transaminase genetically engineered bacterium through construction is capable of increasing intracellular coenzyme PLP content obviously, avoiding adding of externally-sourced PLP in production of L-phosphinothricin using the genetically engineered bacterium, increasing transaminase enzyme activity and stability obviously, prolonging transaminase half life, reducing production cost, and increasing L-phosphinothricin production efficiency.

The invention discloses a culturing method of Lb.plantarum capable of highly producing gamma-aminobutyric acid (GABA) and application, and belongs to the technical field of microorganism fermentation. The method disclosed by the invention includes the steps that: Lb.plantarum ATCC14917 is activated, and the activated Lb.plantarum is cultured in a fermentation system according to the inoculum size of 3%, wherein the culture medium is MRS culture medium, the initial pH value for fermentation is 4.0-6.8, the concentration of L-sodium glutamate is 0-1 mol / L, the addition amount of pyridoxal phosphate is 0-0.001 mol / L, the fermentation temperature is 25-42 DEG C, and the fermentation time is 8-72 h. The method provided by the invention has the characteristics of short culture time, less inoculum size, and high yield of GABA, and has the advantages that the inoculum size is only 3%, the culture time is only 36 h, while the yield of GABA is 360.67 mM / L of fermentation broth, being 7.77 times higher than the control group.

The invention relates to a transaminase-PLP co-immobilized enzyme and a preparation and application thereof, and application of the co-immobilized transaminase for preparing a Sitagliptin medicine chiral midbody. Epoxy resin is adopted as a carrier, and the transaminase-PLP co-immobilized enzyme is obtained by covalence immobilizing of transaminase and coenzyme pyridoxal phosphate (PLP for short).The transaminase-PLP co-immobilized enzyme is adopted as a catalyst, the stability is good, the service life is long, the organic solvent tolerance is good, the transaminase-PLP co-immobilized enzymecan be repeatedly used, no expensive exogenous coenzymes are added in the reaction process, and the production cost is greatly lowered. The method is simple in technology, low in cost, and high in product yield and purity, and the enzyme has the great application value in industrial production of the Sitagliptin medicine chiral midbody.

The invention discloses a method for synthetizing chiral cyclic alkyl amino acid by amino transferase. The commercialized material ketonic acid or corresponding soluble ketonic acid salt compound in the market is selected as an initial material; the initial material is dissolved into phosphate buffer, and added to an amino supply body; pyridoxal phosphate (PLP) and amino transferase main enzyme are added to a system containing the amino supply body and main material ketonic acid or corresponding soluble ketonic acid salt compound to react under constant temperature, and obtaining a product with a high ee value, wherein n is equal to 1, 2, 3, 4, 5, or obtaining a product wherein n is equal to 0 and 1. The method is stable in technological condition, simple to operate, high in yield, low in cost, and suitable for large-scale production, and beneficial for environmental protection; and a novel train of thought and a method are provided for the preparation of chiral cyclic alkyl amino acid compound.

The invention relates to an enterococcus faecalis with the collection number of CGMCC No.3165. A bacterial colony is cultured on an MRS agar culture medium at the temperature of 37 DEG C for 24 hours to be gathered; the bacterial colony is white, oval, is in short chain and Gram-negative, generates acid when fermenting glucose and is elliptic under a common microscope. When being applied, the bacterial colony is selected from a slope to be inoculated into a test tube filled with culture medium A to stand at the temperature of 37 DEG C to be cultured for 24 hours; after being repeatedly activated five times, the finally activated bacteria solution is inoculated into a culture medium B to stand at the temperature of 37 DEG C to be cultured for four days; inoculation amount is 106cfu / ml; cultured bacteria solution is decentralized at 1000 rpm for 10 minutes; and supernate is extracted to obtain high concentration tyramine solution. The culture medium A is an MRS liquid culture medium added with 0.1% of tyrosine, and the culture medium B is an MRS liquid culture medium added with 0.005% of pyridoxal phosphate and 0.1% of tyrosine.

The invention relates to a pretreatment method for extracting allicin. The pretreatment method for an extraction method for allicin comprises the following steps: garlic peeling and cleaning, and homogenizing, wherein pyridoxal phosphate and / or glutathione is added in garlic slurry in the homogenizing step. According to the pretreatment method disclosed by the invention, the yield of extraction for allicin is remarkably increased.

The invention relates to the technical field of enzyme catalysis, and specifically relates to a transaminase catalyst; and the invention further relates to a method for synthesizing (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting an enzymatic way, as well as a production method of the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way comprises a step of, in the presence of pyridoxal phosphate and the transaminase catalyst, allowing reaction of N-tert-butoxycarbonyl-3-piperidinone as a reaction substrate with an amino donor so as to produce the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way utilizes relatively few reagents, and is mild in reaction conditions, so that tedious steps needed for chemical process synthesis are greatly simplified; and moreover, a target product withan ee value up to 99.77% or above can be obtained without performance of separation. Therefore, the transaminase catalyst and the process method utilizing the transaminase catalyst to synthesize the (R)-1-tert-butoxycarbonyl-3-aminopiperidine provided by the invention have broad application prospects as well as great market values.

The present invention relates to methods for regulating the condition of mammalian keratinous tissue wherein the methods each comprise the step of topically applying to the keratinous tissue of a mammal needing such treatment, a safe and effective amount of a skin care composition comprising:a) a safe and effective amount of a vitamin B6 compound selected from the group consisting of pyridoxine, esters of pyridoxine, amines of pyridoxine, salts of pyridoxine and derivatives thereof, including pyridoxamine, pyridoxal, pyridoxal phosphate, and pyridoxic acid;b) a safe and effective amount of a skin care active selected from the group consisting of peptides, phytosterol, sugar amines, their derivatives, and combinations thereof; andc) a dermatologically acceptable carrier for the pyridoxine and the skin care active.

The present invention belongs to the technical field of chiral amine preparations and particularly relates to a preparation method of chiral amine (R)-N1,N1-diethyl-1,4-pentanediamine. A synthesis ofthe (R)-N1,N1-diethyl-1,4-pentanediamine comprises the following steps: using 5-diethylamino-2-pentanone as a raw material and catalyzing the 5-diethylamino-2-pentanone with an amino group donor isopropylamine to conduct a transamination reaction under catalysis of omega-transaminase ATA-117 and coenzyme pyridoxal phosphate to produce the(R)-N1,N1-diethyl-1,4-pentanediamine. The product prepared by the provided method is good in stereoselectivity and produced enantiomer by-products are less than 0.5%; after purification by distillation, purity reaches 99.3% or more and single impurity reachesstarting raw material indexes of raw material medicines; and a total yield can reach as high as 70-85%, the yield is obviously higher than that of traditional process, and the preparation method has avery good industrial application prospect.

The invention discloses a method for preparing an immobilized metal affinity material by using a chelating agent 5-pyridoxal phosphate, and an application of the immobilized metal affinity material in sample pretreatment. The method comprises the following steps: firstly selecting several common nano materials to carry out chemical modification so as to prepare amino modified nano materials to obtain Y-NH2; grafting the 5-pyridoxal phosphate on the Y-NH2 to obtain a surface modified phosphate radical Y-NH2-PLP; and finally immobilizing metal ions on the phosphate radical to obtain Y-NH2-PLP-M<n+>. The modification method of the invention is simple and time-saving, and does not destroy the morphological characteristics of the base material, and the modification is used for realizing the functionalization of the base material, thereby increasing the practicability of the base material. The Y-NH2-PLP-M<n+> can be directly used as extraction material for the sample pretreatment of complex biological samples, the material is separated from the sample in the extraction process through centrifugal or external magnetic field action, and the immobilized metal affinity material has been successfully applied to extraction of phosphorylated polypeptide of standard peptide mixed liquor, milk enzymatic hydrolysate, human serum samples and rat brain lysate.

The invention relates to a supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises the following steps of: culturing a strain with tyrosine phenol lyase activity by using an slant activated inoculum, inoculating the cultured strain against a fermentation culture medium, carrying out fermentation culturing, and collecting resting cells; adding the resting cells into the buffer solution of potassium phosphate, and putting the buffer solution of potassium phosphate into an ultrasonic device; adding phenol, sodium pyruvate, ammonium chloride and pyridoxal phosphate into the resting cells, converting to synthesize, and filtering to obtain resting cells and a product of conversion, and filtering and drying the product of conversion to obtain L-tyrosine, wherein the resting cells are recycled for next reaction. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises has the advantages of simple process, advanced technology, high safety and short production cycle, is suitable for industrial production and has good market prospect.

The invention provides a biological preparation method of gamma-aminobutyric acid, which comprises the following steps of: 1) preparing glutamic acid decarboxylase whole cell thallus by using an E. coli engineering strain capable of expressing glutamic acid decarboxylase; 2) using monosodium glutamate and concentrated hydrochloric acid to prepare L-glutamic acid; 3) dissolving L-glutamic acid as asubstrate in water, adding glutamic acid decarboxylase whole cell thallus and pyridoxal phosphate, stirring and reacting to obtain gamma-aminobutyric acid. The method uses water to replace buffer salt solution to form a water-phase reaction system, uses monosodium glutamate to replace high-purity glutamic acid, so that the purity of the gamma-aminobutyric acid is up to 97.7 percent, and the costof raw materials is reduced; whole cells used for catalysis can be produced in a large scale by microbial fermentation, the cost is low, and the source is wide; the biological enzyme-promoted catalytic reaction is a water-phase reaction, the enzymatic conversion condition is mild, the raw material conversion is thorough, the post-treatment is simple, the product is separated by adopting a method of evaporation concentration and natural crystallization, the cost is low, the process is environment-friendly, and the method is suitable for industrial production of gamma-aminobutyric acid.

The invention provides a method for catalytic synthesis of a sitagliptin chiral intermediate (3R)-3-amino-1-[3-(trifluoromethyl)-5,6,7,8-teralin-1,2,4-triazol[4,3-a]pyrazine-1-yl]-4-(2,4,5-trifluoro-phenyl)buta-1-one by means of engineering bacterium cells containing transaminase-coenzyme pyridoxal phosphate co-immobilization Escherichia coli, E.coli). The method comprises the steps of culturing engineering bacteria containing transaminase Escherichia coli, preparing co-immobilization cells, and synthesizing the immobilization cell asymmetric catalysis itagliptin chiral intermediate. The transaminase-PLP co-immobilization cells are adopted as a catalyst, the stability is good, the service life is long, the organic solvent is good in tolerance, repeated usage can be performed, no expensiveexogenous coenzyme needs to be added in the reaction process, and the production cost is greatly lowered. The method is simple in technology, low in cost and high in product yield and purity, and hasthe quite high application value in the industrial production of the sitagliptin chiral intermediate.

The invention discloses an oxygen carrier based on a hemoglobin-haptoglobin compound and a preparation method of the oxygen carrier. The oxygen carrier comprises haptoglobin, hemoglobin, antioxidant and pyridoxal phosphate. The hemoglobin-haptoglobin compound is formed as a unit for bringing the oxygen carrying activity into play by virtue of special combination ability of the haptoglobin and the hemoglobin. The oxygen carrier based on the hemoglobin-haptoglobin compound disclosed by the invention is free of hypertension reaction after being infused, and capable of protecting an isolated heart. And therefore, the oxygen carrier can be used for clinical blood transfusion, protecting vital organs including the heart and the like from ischemia reperfusion injuries, and has good application prospects and economical benefits.

The invention discloses a method for producing lactic acid and simultaneously realizing coupling preparation of GABA (gamma-aminobutyric acid). The method comprises the steps of inoculating rhizopus oryzae into a fermentation culture medium to perform fermentation, adding glutamic acid decarboxylase, pyridoxal phosphate and L-sodium glutamate into a fermentation solution when the pH value of the fermentation solution is less than 4.8, then continuously adding L-sodium glutamate into the fermentation solution, and generating GABA of glutamic acid decarboxylase by utilizing the vitality of catalyzing glutamic acid to consume H+ in an environment so as to maintain the pH value of the fermentation solution at 4.8-6.0 and achieve the purposes of relieving acid inhibition against fermentation of lactic acid and realizing simultaneous production of lactic acid and GABA. The invention provides a new method for relieving the inhibition of a substrate for fermentation of lactic acid; furthermore, in the fermentation process, the coupling production of a functional compound, namely gamma-aminobutyric acid, is realized, so that the yield of lactic acid is increased, and the economy of the process is further improved.

The object of the present invention is to provide organisms capable of producing ergothioneine which are simpler and less time-consuming than the prior art and which are capable of producing ergothioneine in high yields, thus enabling the production of ergothioneine on an industrial scale Thioneine. The above objects are solved by a transformed filamentous bacterium that has inserted a gene encoding the following enzyme (1) or genes encoding the following enzymes (1) and (2) and overexpressed the inserted gene. (1) In the presence of S-adenosylmethionine, iron (II) and oxygen, catalyze the formation of histidine trimethyl cysteine ​​sulfoxide from histidine and cysteine The enzyme of reaction; (2) with 5 ' - pyridoxal phosphate as coenzyme, catalyzes the enzyme of the reaction that generates ergothioneine by histidine trimethyl cysteine ​​sulfoxide.

The invention relates to a method for synthesizing a key enzyme cofactor by adding daptomycin to improve the daptomycin yield, which comprises the following steps of: putting streptomyces reseosporus slant spores into sterile water to prepare a spore suspension at room temperature; then inoculating the 0.5-ml spore suspension into a 250ml shake flask filled with a 30ml seed culture medium at the temperature of 28-32 DEG C, and cultivating in a 180-220rpm shaking bed for 48 hours; then adding the mixture with a 1-percent inoculated dose into an automatic 7.5LDE fermentation tank filled with a fermentation culture medium; adding one or the composition of 0.5-30mg / l cofactor heme or calcium leucovorin or pyridoxal phosphate VB6; controlling the ventilation volume to be 0.8-1.2v / v / m at the temperature of 28-32 DEG C, and fermenting for 90-144 hours with a pH value of 6.5-7.5. The method is utilized for regulating biological enzyme activity, so that an intracellular metabolic flux leading to daptomycin synthesis is increased, and the yield of the daptomycin is improved; the method has simple operation and low cost, and the fermentation unit of the daptomycin in the fermented cultivated streptomyces reseosporus can be improved by 0.24-4.6 times than a reference substance.