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Method for detecting water-soluble vitamins in blood

A technology for water-soluble vitamins and vitamins, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as high cost, long analysis time, and single detection items

Active Publication Date: 2019-11-05
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of them have problems such as single detection items, slow detection speed, long analysis time, and high cost

Method used

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  • Method for detecting water-soluble vitamins in blood
  • Method for detecting water-soluble vitamins in blood
  • Method for detecting water-soluble vitamins in blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0121] Embodiment 1: prepare the mixed standard solution of series concentration

[0122] Prepare standard song mixed intermediate solution: pipette the same volume of vitamin B1 standard stock solution, vitamin B2 standard stock solution, vitamin B3 standard stock solution, vitamin B5 standard stock solution, pyridoxic acid standard stock solution, pyridoxal phosphate standard stock solution , vitamin B7 standard stock solution, vitamin B9 standard stock solution, 5-methyltetrahydrofolate standard stock solution, vitamin B12 standard stock solution and vitamin C standard stock solution;

[0123] Pipette the vitamin B1 standard stock solution with a concentration of 100 μg / mL, the vitamin B2 standard stock solution with a concentration of 50 μg / mL, the vitamin B3 standard stock solution with a concentration of 100 μg / mL, and the vitamin B5 standard stock solution with a concentration of 1000 μg / mL. solution, pyridoxic acid standard stock solution with a concentration of 100 μg...

Embodiment 2

[0127] Embodiment 2: fitting standard curve equation

[0128] A liquid chromatography-mass spectrometer was used to detect the mixed standard solutions of each concentration obtained in Example 1 above, and the spectra of seven mixed standard solutions with different concentrations were obtained.

[0129] Vitamin B1, vitamin B2, vitamin B3, vitamin B5, pyridoxic acid, pyridoxal phosphate, vitamin B7, vitamin B9, 5-methyltetrahydrofolate, vitamin B12, vitamin C, vitamin B1 isotope, vitamin B2 isotope, vitamin B3 isotope, vitamin B5 isotope, pyridoxic acid isotope, pyridoxal phosphate isotope, vitamin B7 isotope, vitamin B9 isotope, 5-methyltetrahydrofolate isotope, vitamin Chromatographic peak areas of B12 isotope and vitamin C isotope.

[0130] The ratio of the chromatographic peak area of ​​vitamin B1 and the chromatographic peak area of ​​vitamin B1 isotope in the mixed standard solution of above-mentioned seven kinds of different concentrations is used as the ordinate y1 o...

Embodiment 3

[0147] Embodiment 3, processing of blood samples to be tested

[0148] Centrifuge the blood to be tested at a centrifugal speed of 3000-4000RPM for 8-15min, and use the centrifuged supernatant as the blood sample to be tested; pipette the same amount of mixed internal standard working solution as in the mixed standard solution, and add Add the blood sample to be tested into the internal standard working solution, vortex and shake at a speed of 1500-2500RPM for 30s-1min, then add 500μL of 3%-20% sulfosalicylic acid solution, and vortex at a speed of 1200-2500RPM After vortexing and mixing for 5-15 minutes, centrifuge at a high speed of 10000-15000 RPM for 5-15 minutes, and take the supernatant after centrifugation.

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Abstract

The invention provides a method for detecting water-soluble vitamins in blood. The method includes the steps of detecting a mixed standard solution with at least three concentrations through a liquidchromatograph-mass spectrometer to obtain a first detecting result, wherein the mixed standard solution comprises internal standard substances and the water-soluble vitamins, and the water-soluble vitamins include the vitamin B1, the vitamin B2, the vitamin B3, the vitamin B5, pyridoxic acid, pyridoxal phosphate, the vitamin B7, the vitamin B9, 5-methyltetrahydrofolate, the vitamin B12 and the vitamin C; conducting fitting to obtain standard curve equations corresponding to the water-soluble vitamins on the basis of the first detecting result and the concentrations of the water-soluble vitamins; centrifuging a to-be-detected blood sample with a precipitate protein agent and a certain amount of internal standard substances, and taking supernate to be detected through the liquid chromatograph-mass spectrometer to obtain a second detecting result; obtaining the concentration of the water-soluble vitamins in the to-be-detected blood sample on the basis of the standard curve equations and the second detecting result. By means of the scheme, the detecting efficiency of the blood sample can be improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting water-soluble vitamins in blood. Background technique [0002] Water-soluble vitamins are a group of vitamins that can be dissolved in water. They are a type of trace organic substances necessary for the human body to maintain normal physiological functions. Adequate intake can actively promote growth, metabolism, and development. Excessive intake can cause serious harm. Therefore, the detection of water-soluble vitamins in blood is of great significance in clinical medicine. [0003] At present, there are many methods commonly used for the determination of the concentration of water-soluble vitamins in blood, each with its own advantages and disadvantages. Most of them have problems such as single detection items, slow detection speed, long analysis time, and high cost. Contents of the invention [0004] The embodiment of the present inve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/72G01N30/88
CPCG01N30/02G01N30/06G01N30/54G01N30/72G01N30/88G01N2030/027G01N2030/062G01N2030/884
Inventor 赵金宝解娜贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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