Transformed filamentous bacteria with enhanced ergothioneine production capacity and preparation method for ergothioneine

Abstract

The object of the present invention is to provide organisms capable of producing ergothioneine which are simpler and less time-consuming than the prior art and which are capable of producing ergothioneine in high yields, thus enabling the production of ergothioneine on an industrial scale Thioneine. The above objects are solved by a transformed filamentous bacterium that has inserted a gene encoding the following enzyme (1) or genes encoding the following enzymes (1) and (2) and overexpressed the inserted gene. (1) In the presence of S-adenosylmethionine, iron (II) and oxygen, catalyze the formation of histidine trimethyl cysteine ​​sulfoxide from histidine and cysteine The enzyme of reaction; (2) with 5 ' - pyridoxal phosphate as coenzyme, catalyzes the enzyme of the reaction that generates ergothioneine by histidine trimethyl cysteine ​​sulfoxide.

Description

[0001] Cross-referencing of related applications [0002] This application claims the priority of Japanese Patent Application No. 2015-17328 filed on January 30, 2015 and Japanese Patent Application No. 2015-157444 filed on August 7, 2015 , and is disclosed herein by reference in its entirety. technical field [0003] The present invention relates to fungi with approved ergothioneine production capacity and a method for preparing ergothioneine using the fungi. Background technique [0004] The following formula [II]: [0005] [0006] The indicated ergothioneine is a sulfur-containing amino acid, and is a biological substance confirmed to be present in organs such as the liver and blood of animals including humans. [0007] Ergothioneine is known to have antioxidant activity, and has been reported to have, for example, a scavenging action of hydroxyl radicals, an action of inhibiting the production of hydroxyl radicals derived from iron or copper-dependent hydrogen pero...

AI Technical Summary

Problems solved by technology

In addition, in the following non-patent documents 1 and 2 (the entire contents of which are disclosed here by reference), it is described that most bacteria do not have ergothioneine production ability, and it is also described that among fungi, although Aspergillus niger (Aspergillus niger , Aspergillus niger) or Neurospora crassa (Pink bread mold) also have the ability to produce ergothioneine, but Saccharomyces cerevisiae ( Saccharomyces cerevisiae , baker's yeast) have little ability to produce ergothioneine

Images