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Supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine

A tyrosinase method and ultrasonic technology, applied in the biological field, can solve the problems of low efficiency, high cost and long reaction time of L-tyrosine, and achieve the effects of simple process flow, low cost and short production cycle.

Inactive Publication Date: 2015-04-08
NINGBO ZHENHAI HAIDE BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a high-efficiency, environmentally friendly, safe and cost-saving L-tyrosine with supplementary material and ultrasonic assisted in order to solve the defects of low efficiency, long reaction time and high cost in the existing preparation of L-tyrosine. -Tyrosinase conversion preparation method

Method used

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  • Supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine

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preparation example Construction

[0027] The main body of the reaction device of the L-tyrosinase conversion preparation method includes: a feeding device, a reaction device and an ultrasonic treatment device. The feeding device includes a feeding tank 1, a feeding outlet valve 2, and a feeding pump 4; the reaction device includes Exhaust pipe 5, agitator 6, reaction tank 3, discharge valve 10, liquid outlet pump 11, filter 12, liquid outlet valve 13; ultrasonic device includes display 7, control panel 8 and tank body 9, reaction tank 3 is placed In the tank body 9 of the ultrasonic device, there are two feeding tanks 1, phenol is added in one feeding tank 1, and a mixture of sodium pyruvate, ammonium chloride and pyridoxal phosphate is added in the other feeding tank 1. solution.

[0028] The raw materials and strains used in the present invention can be purchased from the market.

Embodiment 1

[0030] A preparation method for feeding and ultrasonically assisted L-tyrosinase conversion, said preparation method comprising the following steps:

[0031] a) Citrobacter freundii Citrobacter freundii ATCC 8090 obtained resting cells after slant-activated seeds were cultured and fermented in the fermentation medium; the fermentation medium was 3 g / L of yeast extract, 1 g / L of L-tyrosine, 1 g / L of dipotassium hydrogen phosphate, Magnesium sulfate heptahydrate 0.5g / L, ferrous sulfate heptahydrate 0.01g / L and pyridoxine hydrochloride 0.05g / L;

[0032] b) Add the resting cells in step a) into the reaction tank, the pH of the potassium phosphate buffer in the reaction tank is 8, 100mM, and the amount of the resting cells is 10 10 CFU / mL, the reaction tank is placed in the tank of the ultrasonic device, and the action time is 15 minutes; the ultrasonic power is 5w, and the temperature is 8°C

[0033] c) Add phenol, sodium pyruvate, ammonium chloride and pyridoxal phosphate in ...

Embodiment 2

[0035] A preparation method for feeding and ultrasonically assisted L-tyrosinase conversion, said preparation method comprising the following steps:

[0036] a) Citrobacter freundii Citrobacter freundii ATCC 8090 obtained resting cells after slant activated seed culture and access to fermentation medium for fermentation; wherein, the fermentation medium was yeast extract 5g / L, L-tyrosine 2g / L, dipotassium hydrogen phosphate 2g / L, Magnesium sulfate heptahydrate 1g / L, ferrous sulfate heptahydrate 0.02g / L and pyridoxine hydrochloride 0.1g / L;

[0037] b) Add the resting cells in step a) into the reaction tank, the pH of the potassium phosphate buffer in the reaction tank is 9, 100mM, and the amount of the resting cells is 10 10 CFU / mL, the reaction tank is placed in the tank of the ultrasonic device, and the action time is 20min; the ultrasonic power is 85w, and the temperature is 10°C

[0038] c) Add phenol, sodium pyruvate, ammonium chloride and pyridoxal phosphate in the fe...

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Abstract

The invention relates to a supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises the following steps of: culturing a strain with tyrosine phenol lyase activity by using an slant activated inoculum, inoculating the cultured strain against a fermentation culture medium, carrying out fermentation culturing, and collecting resting cells; adding the resting cells into the buffer solution of potassium phosphate, and putting the buffer solution of potassium phosphate into an ultrasonic device; adding phenol, sodium pyruvate, ammonium chloride and pyridoxal phosphate into the resting cells, converting to synthesize, and filtering to obtain resting cells and a product of conversion, and filtering and drying the product of conversion to obtain L-tyrosine, wherein the resting cells are recycled for next reaction. The supplementation and enzymatic conversion based ultrasonic-assisted preparation method of L-tyrosine comprises has the advantages of simple process, advanced technology, high safety and short production cycle, is suitable for industrial production and has good market prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of L-tyrosine, in particular to an enzymatic conversion preparation method of L-tyrosin with supplementary materials and ultrasonic assisting. Background technique [0002] L-tyrosine is an aromatic amino acid, one of the important amino acids needed for protein synthesis in animals, and its amino and carboxyl groups have important physiological functions. In the field of medicine, L-tyrosine is the precursor for the synthesis of thyroxine, dopa, and adrenal gland; in the field of food, L-tyrosine is an important additive; clinically, L-tyrosine is also used for reproduction Contraceptive Efficacy Research. According to current literature reports, the preparation methods of L-tyrosine mainly include extraction method, chemical synthesis method, fermentation method and enzymatic conversion method. [0003] Enzymatic conversion has been paid more and more attention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12M1/42C12M1/02C12R1/01
Inventor 王雷缪铭张涛夏小乐李永彪
Owner NINGBO ZHENHAI HAIDE BIOCHEM
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