Heat-resisting and freeze-drying protector for live vaccine and preparation method thereof as well as live newcastle disease vaccine
A technology of heat-resistant freeze-drying and Newcastle disease, which is applied to medical preparations, antiviral agents, and pharmaceutical formulas with non-active ingredients, and can solve the problems of long-term storage and long-distance transportation restrictions, harsh storage conditions, and poor protective performance of vaccines. Achieve the effect of long storage time, stable protection performance and good protection effect
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[0017] 1. Preparation of Live Newcastle Disease Vaccine
[0018] Measure the hemagglutination titer of the chicken Newcastle disease vaccine strain to be 640, dilute it 20 times with sterile normal saline, and insert 0.2ml of the dilution solution of the Newcastle disease vaccine strain into 9-10-day-old SPF chicken embryos under aseptic conditions, and each chicken Embryos were inoculated in the allantoic cavity, sealed with paraffin and incubated in a 36°C incubator for 4-5 days. After the culture was completed, they were placed in a 4°C refrigerator overnight, and then the allantoic fluid was taken out under aseptic conditions. Finally, the hemagglutination titer of the virus was determined to be between 604-1280, and then a 0.45 μ microporous filter membrane was used to filter under pressure.
[0019] 2. Configuration of heat-resistant freeze-drying protective agent
[0020] The protective agent can be divided into two parts: one part is high temperature resistant polyvin...
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