Method for Tissue Culture Regeneration of Mature Bromegrass Seeds

Abstract

The invention discloses a method for tissue culture and regeneration of mature seeds of awnless brome. The mature seeds of awnless brome are used as explants, and the surface is first sterilized, and then the sterilized seeds of awnless brome are transferred to cultured in the callus induction medium; the induced callus was transferred to the first subculture medium for the first subculture, and then transferred to the differentiation medium for culture; the adventitious buds obtained from the differentiation culture were inserted into the second The second subculture was carried out in the subculture medium, and after differentiation and emergence, the seedlings were inserted into the rooting medium to induce rooting. In this method, mature brome seeds are used as explants, and callus is induced, differentiated and rooted by adding different concentrations of auxin and cytokinin in the medium, so as to establish its complete tissue culture regeneration system. Wheat provides the basis for genetic improvement through genetic engineering.

Description

technical field

[0001] The invention belongs to the field of agricultural biotechnology, in particular to a method for culturing and regenerating mature brome seed tissue. Background technique

[0002] Bromus inermis is a perennial herbaceous plant belonging to the genus Bromus in the grass family (Gramineae). It has the characteristics of strong adaptability, high nutritional value, large yield, long utilization season, and strong regeneration. It is an excellent forage grass. Its wild species are widely distributed in various provinces and autonomous regions of my country, and it is the dominant grass species in the 1000-3500m mountain meadow. Because of its well-developed underground rhizomes and strong drought resistance, it is also a pioneer plant for establishing artificial pastures and environmental sand fixation.

[0003] In recent years, with the adjustment of the industrial structure and the demand for environmental governance, the artificial planting area of ​​b...

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