Gene encoding hydrogen peroxide fluorescent probe, preparation method and applications thereof
A technology of fluorescent probes and hydrogen peroxide, which is applied in chemical instruments and methods, fluorescence/phosphorescence, and the introduction of foreign genetic material using carriers, etc., which can solve the problems of limiting dynamic changes and small fluorescence
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[0044] I. Definition:
[0045] As used herein, the term "about" when a value or range is given means that the value or range is within 20%, within 10%, and within 5% of the given value or range.
[0046] As used herein, the terms "comprising", "comprising" and their equivalents include the meanings of "containing" and "consisting of", for example a composition "comprising" X may consist solely of X or may contain other substances, such as X+ Y.
[0047] In the present invention, the term "HyPer" refers to a genetically encoded hydrogen peroxide fluorescent probe that has been reported to insert the cpYFP protein into OxyR-RD, which is connected by a short chain of amino acids in the middle.
[0048] The term "HyPer protein mutant" or "HyPer mutant" or "rationally designed HyPer mutant" as used herein refers to introducing a mutant site respectively at the corresponding site on the HyPer probe cpYFP element, and then increasing the fluorescence The mutants were continuously i...
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