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This invention relates to the field of plant breeding technology, and particularly to SNP loci related to the oil content of Camellia oleifera seed kernels in Hainan and their applications. The SNP loci include one or more of Chr27_83344296, Chr43_56056639, or Chr15_65268299; Chr27_83344296 is located at position 83344296 on chromosome 27, with a polymorphism of G / T; Chr43_56056639 is located at position 56056639 on chromosome 43, with a polymorphism of C / T; and Chr15_65268299 is located at position 65268299 on chromosome 15, with a polymorphism of G / A. By detecting the polymorphism of these SNP loci, the oil content of Camellia oleifera seed kernels in Hainan can be detected at the seedling stage, significantly reducing production costs and improving selection efficiency, thus possessing significant application value.