3results about How to "Purposeful" patented technology

The invention provides a development method and application of a novel molecular marker, and belongs to the technical field of molecular markers. The invention provides a molecular marker development method, according to a significant variation site provided by a GWAS result, a common primer is designed near the significant variation site, a new variation site is found through Sanger sequencing, and genetic typing, correlation analysis and the like are carried out by utilizing a molecular biological technology for verification, so that a brand new molecular marker is obtained. According to the method, the number of variation sites is large, the requirement for experimental equipment is relatively low, the experimental process is relatively simple, result guiding is more accurate, purposiveness is higher, and developed molecular markers are also remarkable. The brand new molecular marker screened by the method provided by the invention provides an experimental basis for genetic breeding of HuaMongolian mutton sheep, and provides a new idea for accelerating breeding of new sheep varieties.

The invention relates to a coating debugging and processing control method, which comprises the following steps: S1. injecting resin raw material and solvent into a reactor and continuously stirring, and recording the injected mass of the resin raw material as m, and recording the injected mass of the solvent as n; S2. injecting an emulsifier into the reactor through a first feeding mechanism and continuously stirring, and recording the injected mass of the emulsifier as a; S3. injecting a wetting dispersant into the reactor through a second feeding mechanism and continuously stirring, and recording the injected mass of the wetting dispersant as b; S4. injecting a defoaming agent into the reactor through a third feeding mechanism and continuously stirring, and recording the injected mass of the defoaming agent as c; S5. injecting a pigment into the reactor through a fourth feeding mechanism and continuously stirring, and recording the injected mass of the pigment as d; the method performs performance monitoring item by item according to the properties of different raw materials, the monitoring has a strong purpose, and the method of adding raw materials item by item can achieve high-precision adjustment of the raw material ratio.

This invention relates to the field of plant breeding technology, and particularly to SNP loci related to the oil content of Camellia oleifera seed kernels in Hainan and their applications. The SNP loci include one or more of Chr27_83344296, Chr43_56056639, or Chr15_65268299; Chr27_83344296 is located at position 83344296 on chromosome 27, with a polymorphism of G / T; Chr43_56056639 is located at position 56056639 on chromosome 43, with a polymorphism of C / T; and Chr15_65268299 is located at position 65268299 on chromosome 15, with a polymorphism of G / A. By detecting the polymorphism of these SNP loci, the oil content of Camellia oleifera seed kernels in Hainan can be detected at the seedling stage, significantly reducing production costs and improving selection efficiency, thus possessing significant application value.