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Method for obtaining temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system

The invention discloses a method for obtaining a temperature-sensitive sterile line by performing site-specific mutagenesis on P/TMS12-1 through a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system. The method comprises the following steps: cloning and controlling a Pei'ai 64S temperature-sensitive sterile major gene P/TMS12-1 fragment; designing a target sequence according to the P/TMS12-1 sequence; constructing a pU3-gRNA carrier of the target-containing sequence fragment; constructing a pCRISPR/Cas9 carrier; obtaining a positive transgenic seedling by utilizing the pCRISPR/Cas9 carrier containing the target sequence fragment; screening a mutant plant from the positive transgenic seeding; performing subculture planting on the mutant plant to obtain the temperature-sensitive sterile line without transgenic components. According to the method disclosed by the invention, the CRISPR/Cas9 system is utilized to completely inactivate P/TMS12-1 non-coding RNA, and the temperature-sensitive sterile line without transgenic components is artificially cultivated. The method disclosed by the invention has the advantages of being strong in purposiveness, small in genome damages and capable of avoiding transgenic interference.
Owner:SOUTH CHINA AGRI UNIV

Method for controlling construction quality of asphalt pavement in real time

The invention discloses a method for controlling construction quality of an asphalt pavement in real time, which comprises the following steps of: 1, drawing a universal volumetric chart to evaluate volumetric parameter characteristics of various asphalt mixtures, and selecting a proportion of an asphalt mixture, which is suitable for the local environment and is difficult to segregate; 2, checking that whether volumetric parameter information of the asphalt mixture produced in the day meets requirements of design and construction specifications so as to quickly regulate the production process; 3, evaluating the aggregation condition of the asphalt pavement by using the universal volumetric chart; and 4, analyzing and judging the compaction degree condition and temperature condition of the asphalt pavement, judging the compaction qualified rate, judging the significance of the density data difference of the asphalt pavement, checking the construction quality of the asphalt pavement and providing quantitative basis for later period payment adjustment. The method has the advantages of reasonable design, high reliability, high operability, high real-time property, high efficiency of evaluating the construction quality of the asphalt pavement, and high practicability, and contributes to improving construction quality and saving economic cost.
Owner:XINJIANG BEIXIN ROAD & BRIDGE GRP +1

Method for massively and efficiently developing molecular markers on basis of Indel and SSR (simple sequence repeat) site techniques

The invention discloses a method for massively and efficiently developing molecular markers on the basis of Indel and SSR (simple sequence repeat) site techniques. The method comprises the following steps: (1) selecting at least 3 samples to be developed, and respectively extracting DNAs (deoxyribonucleic acids) of the samples to be developed; (2) carrying out enzyme digestion on the DNA samples of the samples to be developed, establishing a sequencing library, and carrying out sequencing; (3) mixing the genomes of all the samples to be developed, and assembling to obtain Contigs; (4) comparing the Contigs with the sequences of the sample individuals to be developed, and acquiring SSR sites with Indel inside according to the Indel and SSR site information as a candidate polymorphism SSR site; (5) designing primers according to the obtained candidate polymorphism SSR site, carrying out PCR (polymerase chain reaction) amplification and sequencing, and selecting a ribbon-shaped stable clear strip as molecular marker primers to be verified; and (6) carrying out PCR amplification on different samples to be developed by using the obtained molecular marker primers, and selecting the molecular markers with diversity, thereby obtaining the molecular markers. The method enhances the molecular marker development efficiency; and the developed SSR molecular markers can be efficiently applied to research in the aspects of genetics, multiplication release evaluation and the like.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Method of preparing instant nursing barley green product through using hot-air dehydrated barley grass powder as raw material

The invention discloses a method of preparing an instant nursing barley green product through using hot-air dehydrated barley grass powder as a raw material, and belongs to the technical field of research and development of health care foods. The method comprises the following steps: taking the hot-air dehydrated barley grass powder as the main raw material, adding other nutrient substances and food fillers (such as maltodextrin and beta-cyclodextrin) as processing auxiliary materials to produce the instant nursing barley green health care product, and specifically comprises the steps of extraction, concentration, mixing, pH value regulation, drying, packaging and the like. Compared with the conventional extraction method, the nutrients are extracted by a double-frequency ultrasonic wall breaking method from the hot-air dehydrated barley grass powder; the method is short in extraction time, high in nutrient extraction ratio and low in loss; the auxiliary materials can be added according to demands of different users, so that the quality of the barley green product can be improved, the product can achieve a better pertinence, and consumers can purchase the product with more accurate purposes; a low-temperature microwave vacuum spray drying method is adopted, so that the method is short in drying time, and low in damage to thermosensitive substances; the product is fine and smooth, and good in solubility.
Owner:NINGBO HAITONG FOOD SCI & TECH CO LTD +1

Method for separating and de-noising wave fields of longitudinal and transverse earthquake waves

The invention discloses a method for separating and de-noising wave fields of longitudinal and transverse earthquake waves. The method comprises the following steps of: reading earthquake data of a sampling point into a two-dimensional array; performing normal time difference correction on the primary earthquake data; decomposing singular values of the earthquake data after the primary normal time difference correction; extracting singular value reconstruction signals of target signals; performing normal time difference back correction on the reconstructed data; outputting the data after the normal time difference back correction according to an earthquake data format during inputting so as to realize earthquake P-P wave separation and de-noising; performing normal time difference correction on earthquake records after the P-P wave is separated; decomposing singular values of the earthquake records after the secondary normal time difference correction; extracting the singular value reconstruction signals of the target signals; performing normal time difference back correction on the reconstructed data; outputting the data after the normal time difference back correction according to the earthquake data format during inputting so as to realize earthquake P-S wave field separation and de-noising; and outputting the data after the normal time difference back correction according to the earthquake data format during inputting. The method has the characteristic of thoroughly separating the longitudinal and transverse earthquake waves.
Owner:XI'AN PETROLEUM UNIVERSITY

Manufacturing method for lifting guide rail of side window glass of automobile

The invention relates to a manufacturing method for a lifting guide rail of a piece of side window glass of an automobile. The method comprises the following steps: selecting a midpoint connected with any point on the glass to be applied for serving as a tangent plane of the glass; extracting the characteristic line of a column B; connecting the characteristic line into a straight line by taking the midpoint in the tangent plane as an original point, and forming a normal plane of the straight line on the midpoint; projecting the characteristic line on the normal plane to obtain a projection line, forming a circle by selecting any three points from the projection line, and forming a cylindrical face; changing included angles between the midpoint of the tangent plant and the straight line of the characteristic line as well as between the midpoint of the tangent plant and the characteristic line to make intersecting lines fit the characteristic line of a boundary virtual guide rail, which is opposite to the column B, of the glass, and forming a spiral line fitting the characteristic line of the column B; forming a spiral line of the same thread pitch on another boundary according to the previous spiral line, wherein the two spiral lines are taken as lifting lines of the glass; manufacturing the glass lifting guide rail according to the lifting lines.
Owner:ANHUI JIANGHUAI AUTOMOBILE GRP CORP LTD

Polysaccharide extract of Chinese caterpillar fungi as well as preparation method and application of polysaccharide extract

The invention belongs to the field of biotechnology and in particular relates to a polysaccharide extract of Chinese caterpillar fungi as well as a preparation method and application of the polysaccharide extract. The preparation method of the polysaccharide extract of the Chinese caterpillar fungi comprises the following steps: after submerged fermentation culture of mycelium of the Chinese caterpillar fungi, removing the mycelium of a fermented product by virtue of centrifugal separation to obtain a fermentation broth; after removing small molecule substances of the fermentation broth by virtue of ultrafiltration, concentrating the fermentation broth; and carrying out treatments of protein removal, ethanol precipitation, decoloring by hydrogen peroxide, dialysis and vacuum freeze-drying on the concentrated fermentation broth to prepare the polysaccharide extract of the Chinese caterpillar fungi. The preparation method is simple in process and is capable of carrying out efficient mass production with high quality. The prepared polysaccharide extract of the Chinese caterpillar fungi is nonhazardous to a human body, has good physiological function of reducing blood fat and can be used for developing a medicament or health food with the capability of reducing blood fat.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Charge indicator of high-voltage power transmission line

The invention provides a charge indicator of a high-voltage power transmission line. The charge indicator comprises an electricity obtaining unit, an adjusting unit and an indicating unit, wherein the electricity obtaining unit is arranged on the high-voltage power transmission line and obtains electricity from the high-voltage power transmission line in a sensing mode, the input end of the adjusting unit is electrically connected with the output end of the electricity obtaining unit, the adjusting unit is used for outputting stable direct-current electricity, and the indicating unit is electrically connected with the output end of the adjusting unit and is used for charge indication of the high-voltage power transmission line. According to the charge indicator of the high-voltage power transmission line, the whole device is powered up in an isolating electricity obtaining mode, the charge indicator has no contacts with the high-voltage power transmission line, and safety potential risks are avoided. The charge indicator can clearly indicate that whether the high-voltage power transmission line is charged or not, can send out fault information timely when the high-voltage power transmission line has a fault, can indicate the position where the fault appears, enables overhaul personnel to have a strong purpose in the overhaul process, facilitates timely elimination of the fault, improves work efficiency, and saves manpower.
Owner:STATE GRID CORP OF CHINA +1

Power system additional wide-area damping controller design method giving consideration to time lag

The invention discloses a power system additional wide-area damping controller design method giving consideration to time lag, and the method comprises the steps: (1), enabling an oscillation mode with the weakest damping of a power system to serve as a target control mode, determining the optimal installation positions of AVR and FACTS equipment, further building a power system mode and a WADC model which comprise the AVR and FACTS equipment and do not give consideration to time lag; (2), introducing a time lag link on the basis of the power system mode and the WADC model which comprise the AVR and FACTS equipment and do not give consideration to time lag, and further building a closed loop time lag power system model giving consideration to a time lag factor; (3), building a WADC parameter-optimization mathematic model according to the closed loop time lag power system model; (4), employing a PSO algorithm, and solving the optimal parameters of WADC at step (3). The method gives consideration to the time lag factor in each iteration optimization process, employs the SOD algorithm to solve the key characteristic value of the right side of a closed loop time lag power system, so as to achieve a big purpose that the damping of the key characteristic value is the biggest.
Owner:SHANDONG UNIV

Gas-containing-coal multistage permeability increasing method

The invention discloses a gas-containing-coal multistage permeability increasing method. The gas-containing-coal multistage permeability increasing method includes the steps that firstly, the geological conditions of a coal seam hosting region, the coal metamorphism degree, the coal class and gas parameters are determined; secondly, according to whether a coal seam is located at a fault portion or a fold portion, different schemes are adopted to achieve first-stage permeability increasing of gas containing coal and detect the permeability increasing effect, and if a preset effect is achieved, permeability increasing of the whole coal seam is carried out with the first-stage permeability increasing scheme; thirdly, if the preset effect is not achieved, on the basis of the first-stage permeability increasing, second-stage acidification permeability increasing is carried out on the gas containing coal; finally, after the second-stage acidification permeability increasing is completed, gas extraction is carried out on the gas containing coal. According to the gas-containing-coal multistage permeability increasing method, different permeability increasing schemes are formulated according to the geology of different coal-seam hosting regions, and high pertinence is achieved; meanwhile, after multistage permeability increasing is adopted, the gas permeability of the coal seam is greatly improved, and the gas extraction rate is increased.
Owner:田成林
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