132 results about "Subcellular membrane" patented technology

The present invention provides systems, methods, and compositions for targeted delivery of nanoparticles and/or agents to tissues, cells, and/or subcellular locales. In general, compositions comprise a nanoparticle (e.g. quantum dot, polymeric particle, etc.), at least one modulating entity (such as a targeting moiety, transfection reagent, protective entity, etc.), and at least one agent to be delivered (e.g. therapeutic, prophylactic, and/or diagnostic agent). The present invention provides methods of making and using nanoparticle entities in accordance with the present invention.

Targeted therapeutics that localize to a specific subcellular compartment such as the lysosome are provided. The targeted therapeutics include a therapeutic agent and a targeting moiety that binds a receptor on an exterior surface of the cell, permitting proper subcellular localization of the targeted therapeutic upon internalization of the receptor. Nucleic acids, cells, and methods relating to the practice of the invention are also provided.

Targeted therapeutics that localize to a specific subcellular compartment such as the lysosome are provided. The targeted therapeutics include a therapeutic agent and a targeting moiety that binds a receptor on an exterior surface of the cell, permitting proper subcellular localization of the targeted therapeutic upon internalization of the receptor. Nucleic acids, cells, and methods relating to the practice of the invention are also provided.

The present invention provides methods and compositions related to the fields of chemoinformatics, chemogenomics, drug discovery and development, and drug targeting. In particular, the present invention provides subcellular localization signals (e.g., chemical address tags) that influence (e.g., direct) subcellular and organelle level localization of associated compounds (e.g., drugs and small molecule therapeutics, radioactive species, dyes and imagining agents, proapoptotic agents, antibiotics, etc) in target cells and tissues. The compositions of the present invention modulate the pharmacological profiles of associated compounds by influencing the compound's accumulation, or exclusion, from subcellular loci such as mitochondria, endoplasmic reticulum, cytoplasm, vesicles, granules, nuclei and nucleoli and other subcellular organelles and compartments. The present invention also provides methods for identifying chemical address tags, predicting their targeting characteristics, and for rational designing chemical libraries comprising chemical address tags.

In vitro culture devices and methods are described. The subject methods and devices provide a means whereby cells and/or subcellular material are grown or held in a culture device that maintains the cells and/or subcellular material in a physiologically representative environment, thereby improving the predictive value of toxicity and metabolism assays, and the relevance of experimental results derived from such assays to actual in vivo conditions, processes and outcomes. The culture devices of the invention comprise a fluidic channel connected to or otherwise integrated with at least one chamber, preferably integrated in a chip format. The specific chamber geometry is designed to provide cellular interactions, liquid flow, and liquid residence and other parameter values that correlate with those found in or produced by the corresponding cell, organs or tissues, or components or products thereof, in vivo. Each device comprises at least one chamber and at least one inlet and one outlet port that allow for recirculation of the culture medium. The device will usually include a mechanism for obtaining signals from the cells and culture medium.

The present invention is directed to a method and apparatus for detecting and analyzing cell migration. More specifically, the present invention is directed to novel technology for analyzing cellular movement, including whole cell migration and subcellular component movement. Cells are distributed onto a substrate and monitored for migration or movement. According to one embodiment, when a labelled cell or portion of a cell passes over one of the delineations between detection units, such as individual fibers in a fiber optic bundle, the label causes a large intensity increase, which stays for a given “residence time” until the cell departs from the detection unit.

A system for analyzing tissue samples, comprising: a storage device for at least temporarily storing one or more images of one or more cells, wherein at least one of the images is indicative of one or more channels comprising a receptor tyrosine kinase (RTK); and a processing device that determines an extent to which one or more of the RTKs may have translocated from at least one subcellular region to another subcellular region of one or more of the cells; and generates a score based at least in part on the RTK translocation.

The present invention provides methods for treating or preventing portal hypertension comprising administering to a patient in need of such treatment or prevention at least one Rev-erb modulating agent (REMA) in an amount effective to treat or prevent said portal hypertension. The REMA can be SR6452 or a related compound, such as SR9009 or SR9011. The amount of the REMA administered can comprises a quantity sufficient to modulate Rev-erbα expression, activity, and/or subcellular location and/or to inhibit or reverse hepatic stellate cell (HSC) activation and contractility.

Electrophoretic separation methods and systems for performing the same are provided. The methods and systems find use in a variety of different electrophoretic separation applications, such as sub-cellular Western blotting of single cells.

A compound comprising, in combination: a cell surface binding ligand or internalizing factor, such as an IL-13Rα2 binding ligand; at least one effector molecule (e.g., one, two, three or more effector molecules); optionally but preferably, a cytosol localization element covalently coupled between said binding ligand and said at least one effector molecule; and a subcellular compartment localization signal element covalently coupled between said binding ligand and said at least one effector molecule (and preferably with said cytosol localization element between said binding ligand and said subcellular compartment localization signal element). Methods of using such compounds and formulations containing the same are also described.

The invention provides a high content image flow biological microscopic analysis system. The system comprises a liquid flow system and an optical system, wherein the liquid flow system enables cells in sample cell suspension to be focused in the center of a liquid flow under the constraint of the system sheath liquid flow and to flow through a detection window of a flow chamber one by one; the optical system comprises optical sources, an optical path system and an optical filter stack; the optical sources include a halogen lamp and a laser; through the optical path system and the optical filter stack formed by a plurality of dichroscopes, the optical system generates a bright field cell image, a six-channel fluorescent cell image and six PMT (photomultiplier tube) optical intensity signals with different wave bands. The high content image flow biological microscopic analysis system integrates flow cytometry and fluorescence microscopic imaging, has a plurality of detection channels, can collect the cell images of the cells passing through the flow chamber one by one and can carry out quantitative analysis on each cell image by utilizing analysis software, thus providing the statistical data of a cell group and the information of the cell morphology, cell structures and subcellular signal distribution.

Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between subcellular copartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing β-galactosidase fragments are provided. These β-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided.

Affinity separation compositions and methods are disclosed for separating targets from complex mixtures. Affinity reagents are bound to a solid support oriented in a manner to facilitate the activity of the affinity reagents which are capable of binding specific targets by affinity recognition. Affinity reagents include IgY antibodies, proteins, peptides, nucleotides and polymers. Targets include proteins, protein-protein complexes, protein-nucleotide complexes, nucleotides, cells and subcellular organelles.

Optical detection techniques for the assessment of the physiological state, health and/or viability of biological materials are provided. Biological materials which may be examined using such techniques include cells, tissues, organs and subcellular components. The inventive techniques may be employed in high throughput screening of potential diagnostic and/or therapeutic agents.

The present invention relates to a kind of magnetic separating column and its application in separating biological samples. The magnetic separating column has structure comprising a material holding section, a filler column, a discharge port and a pushing plug. The filler column is filled with small magnetic soft iron balls of sizes 0.01-0.1mm, 0.1-0.2mm and 0.2-1mm separately. During separation, the sample fed through the inlet in the upper end is made to flow through the filler column and the discharge port under the gravity action, and the pushing plug applies pressure to push out the blocked sample. Under the action of outer magnetic field, the magnetic separating column can generate high gradient magnetic field for effective fast separation of various kinds of cells, subcellular matters, bacteria, viruses, nucleic acids, proteins and other biological samples.

The present invention relates to pH-tunable, highly activatable multicolored fluorescent nanoplatforms and methods of using the nanoplatforms in a variety of applications including, but not limited to, investigating fundamental cell physiological processes such as pH regulation in endocytic vesicles, endosome/lysosome maturation, and effect of pH on receptor cycling and trafficking of subcellular organelles.

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

The present invention provides proteins comprising immunoglobulin-type binding regions for cell-type specific targeting and Shiga toxin A Subunit effector regions for Shiga toxin effector functions (e.g. cellular internalization, directing subcellular routing, and/or cytotoxicity), wherein the binding regions and Shiga toxin effector regions are combined such that the Shiga toxin effector regions are proximal to the amino-terminals of the proteins. The presently disclosed proteins can comprise additional exogenous materials, such as, e.g., antigens, cytotoxic agents, and detection-promoting agents, and are capable of targeted delivery of these additional exogenous materials into the interiors of target cells. The proteins of the present invention have uses in methods such as, e.g., methods involving targeted killing of target cells, delivering exogenous materials into target cells, labeling subcellular compartments of target cells, and diagnosing and/or treating a variety of conditions including cancers, tumors, other growth abnormalities, immune disorders, and microbial infections.