Cryopreservation of cells and subcellular fractions

a cell and subcellular technology, applied in the field of cryopreservation of self-sustaining bodies, can solve the problems of limiting the ability to conduct experiments using such a system, and affecting the viability of cells after thawing
US20110105359A1Inactive Publication Date: 2011-05-05XENOTECH CALIFORNIA
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
XENOTECH CALIFORNIA
Publication Date
2011-05-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides cryopreserved compositions of cells, wherein the compositions are advantageously in the form of self-sustaining bodies that can be individually handled and combined independently of a container, allowing for easy customization of the eventual pooled preparation. The invention also provides pre-pooled stacks of the self-sustaining cryopreserved compositions for eventual thawing to produce pooled preparations of cells. A mold and methods for forming the self-sustaining bodies are also provided. The invention is also concerned with methods of forming pooled preparations of cells using single-cryopreserved compositions of cells.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present application claims the benefit of and priority from U.S. Provisional Patent Application Ser. No. 61 / 340,259, filed Mar. 15, 2010, and U.S. Provisional Patent Application Ser. No. 61 / 256,833, filed Oct. 30, 2009, the entire disclosures of which are hereby incorporated by reference herein.BACKGROUND OF THE INVENTION

[0002] 1. Field of the Invention

[0003] The present invention is broadly concerned with cryopreserved self-sustaining bodies formed from compositions of cells, methods of forming the same, and methods of using the same to produced pooled preparations of cells.

[0004] 2. Description of Related Art

[0005] Hepatocytes are parenchymal liver cells, and make up 60-80% of the cytoplasmic mass of the liver. Hepatocytes play a key role in the detoxification, modification, and excretion of exogenous and endogenous substances. One of the detoxifying functions of hepatocytes is to modify ammonia to urea for excretion. They are also impo...

Claims

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