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Cell cryopreservation fluid

A cryopreservation solution and cell technology, applied in the field of cell cryopreservation solution, can solve problems such as instability, virus infection, immune rejection, etc., and achieve good results and clear ingredients

Inactive Publication Date: 2016-09-28
SHENZHEN HORNETCORN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal-derived serum has the risk of infection with pathogens such as viruses and bacteria, and some of the proteins in it may cause immune rejection
In addition, serum has problems such as batch-to-batch variation and instability

Method used

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  • Cell cryopreservation fluid
  • Cell cryopreservation fluid
  • Cell cryopreservation fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The basic ingredient is D / F12 (Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM / F-12)) basal medium, and the added ingredients are as follows:

[0028] Chinese name

Embodiment 2

[0030] The basic ingredient is D / F12 basal medium, and the added ingredients are as follows:

[0031] Chinese name

Embodiment 3

[0033] The basic ingredient is D / F12 basal medium, and the added ingredients are as follows:

[0034] Chinese name

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PUM

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Abstract

The invention discloses a cell cryopreservation fluid which can be used for performing cryopreservation on mesenchymal stem cells or other cells. The cell cryopreservation fluid is prepared from the following ingredients: PBS or normal saline or a basal culture medium serving as a main ingredient, as well as one or more of polyethylene glycol, propanediol, Ectoin, albumin, trehalose, proline and poloxamer 188 which serve as additive ingredients. The cell cryopreservation fluid disclosed by the invention does not contain serums and DMSO, have the definite additive ingredients, and is controllable in quality, high in batch stability and high in safety. By virtue of preserving cells with the cell cryopreservation fluid, revived cells are high in survival rate, and the cellular morphology, the multiplication capacity and the differentiation potential are not influenced; the cell cryopreservation fluid can be used for replacing cryopreservation fluids containing the serums and the DMSO.

Description

Technical field [0001] The invention relates to the field of cells and bioengineering, specifically a cell cryopreservation solution. Background technique [0002] Stem cells have been widely used in scientific research and clinical treatment due to their potential for self-renewal and multi-lineage differentiation. Such as embryonic stem cells, induced pluripotent stem cells and mesenchymal stem cells. Stem cells have a variety of uses and functions. They can be used to study the growth and development of tissues and organs in vitro, establish disease models, screen drugs, and can also be used for clinical treatment. Mesenchymal stem cells among stem cells have been widely used in therapeutic research in animal disease models and human clinical trials. Diseases that can be treated with mesenchymal stem cells include diabetes, liver cirrhosis, skin damage, myocardial infarction, and nerve damage. In addition, mesenchymal stem cells also have functions such as promoting he...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 刘聪黄馨萍陈晨魏志璋罗晓玲
Owner SHENZHEN HORNETCORN BIOTECH
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