15 results about "Chemical library" patented technology

Provided herein are compositions comprising a particle comprising a library encoded bead (e.g., DEL bead) coated in a polymeric matrix forming a core-shell particle, wherein the core-shell particle is further inside a 3D tissue culture. Also provided herein are methods of making such particles, and methods of using such particles for drug discovery such as identification of bioactive compounds via high-throughput cellular activity-based phenotypic screens of DNA-encoded chemical library beads.

The application discloses a kind of chemical warehouse fire accident wastewater unpowered control and collection system, mainly including fire accident wastewater collection well, wastewater valve control well, rainwater valve control well, fire wastewater ditch, outdoor wastewater pipeline, wastewater diversion well, accident pool.It is characterized by: system utilizes hydraulic power from fire accident wastewater collection well, wastewater valve control well, rainwater valve control well Joint automatic control different function valve in wastewater diversion well opening and closing;Normal working condition, wastewater diversion well inside accident pool one side valve is closed, rainwater pipe one side valve is opened, fire condition, wastewater diversion well inside accident pool one side valve is opened, rainwater pipe one side valve is closed.The application solves the current chemical warehouse fire accident wastewater collection system needs manual switching valve in diversion well, causes heavy amount of repeated labor, cannot guarantee timely switching and other safety problems.Simultaneously system composition and working principle are simple, easy to construction installation, easy to large-scale use and promotion.

We provide a system for screening compounds. [Solution] This application provides beads with covalently attached compounds and covalently attached DNA barcodes, as well as methods for using such beads. The beads have many substantially identical copies of compounds and many substantially identical copies of DNA barcodes. The compounds consist of one or more chemical monomers, and the DNA barcodes take the form of barcode modules, each module enabling the identification of corresponding and corresponding chemical monomers. The nucleic acid barcodes may have a chain-like or orthogonal structure. Methods for sequencing bead-bound nucleic acid barcodes, methods for cleaving compounds from beads, and methods for evaluating the biological activity of released compounds are provided.

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are a method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

This disclosure provides methods for screening DNA-encoded chemical libraries using mirror images of target molecules. These methods allow for the efficient selection of compounds that bind to target molecules, even when the target molecules are able to bind to the DNA tag of the encoded compound.

The invention belongs to the field of computer-aided drug design, and discloses a metal binding group extraction method based on chemical informatics analysis and modeling. The method comprises the following steps: constructing a metal binding compound data set; training and optimizing a metal combined compound prediction model by using a decision tree-based model and a graph neural network algorithm; using an SHAP interpretable method to analyze a relationship between the key features and a prediction result, and deriving a structure fragment with potential metal binding capacity; according to the attention score of the Attentive FP model, an atomic weight heat map of molecules in the test set is drawn, and key metal binding fragments are determined; molecules in the training set are fragmented, and representative substructures are extracted; a metal binding fragment chemistry library is compiled. According to the method, the problem that metal binding group extraction is limited to a compound structure data set is solved, efficient extraction of any metal ion binding group is realized, and discovery and research of metalloenzyme-related drugs are accelerated.

Organic compounds for target identification, drug discovery, chemical library production, high-throughput screening, fluorophore conjugation, chemiluminescent compound conjugation, creation of proximity induced modulators (e.g., protein degraders) / chimeric molecules, or a combination thereof are described. The compounds can contain small molecule moieties for identification of their potential targets; an isocyanate, photoactivatable groups; chemical moieties for enrichment and detection of target-small molecule moiety interactions; proximity induced modulator element; fluorophores; chemiluminescent groups; or combinations thereof.

The application discloses a kind of chemical warehouse fire accident wastewater automatic control and collection system, mainly including electrical control cabinet, fire accident wastewater signal detection well, fire wastewater ditch, outdoor wastewater pipeline, wastewater diversion well, accident pool.It is characterized by: electrical control cabinet receives fire accident wastewater signal detection well signal, automatically controls and opens and closes different electric valves in the wastewater diversion well connected with outdoor wastewater pipeline, outdoor rainwater pipeline, accident pool;Normal working condition, the valve on the side of accident pool in wastewater diversion well is closed, the valve on the side of rainwater pipe is opened, fire condition, the valve on the side of accident pool in wastewater diversion well is opened, the valve on the side of rainwater pipe is closed.The application solves the safety problems that chemical warehouse fire accident wastewater collection system needs manual switching of valve in diversion well, causes heavy amount of repeated labor, cannot guarantee timely switching, etc.At the same time, system composition and working principle are simple, easy to construction installation, easy to large-scale use and popularization.

The present invention relates to an electrode array comprising two-electrode assemblies and a corresponding device for use in performing electrochemical synthesis. The invention further relates to use of the device in the high-throughput electrochemical synthesis of chemical compounds, particularly for the purpose of chemical library synthesis and chemical reaction discovery.

The present application provides beads bearing covalently bound compounds and covalently bound DNA barcodes, as well as methods for using such beads. The beads bear multiple copies of substantially identical compounds and multiple copies of substantially identical DNA barcodes. The compounds are composed of one or more chemical monomers, and the DNA barcodes take the form of barcode modules, each corresponding to a corresponding chemical monomer and enabling its identification. The nucleic acid barcodes can have linked or independent structures. Methods are provided for sequencing the bead-bound nucleic acid barcodes to cleave the compounds from the beads and evaluate the biological activity of the released compounds.

The present document relates to headpieces for DNA-encoded chemical libraries and processes for preparing them, to DNA-encoded chemical libraries and DNA-supported pharmacophores or control molecules comprising the headpiece, and to uses thereof. The present document further relates to a method for generating multidimensional selection maps for a DNA-encoded library (DEL) (e.g., double-stranded or single-stranded DEL) against a single target.

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

Methods of synthesizing and screening nanoscale high-throughput chemical libraries for compounds that modulate cellular activity. In one aspect, is a synthesis and high-throughput screening method for a compound that affects at least one biological property in a biological assay comprising the steps of: performing a first reaction (R-I) in each well of a plurality of wells of a microplate to produce a different compound (C-I) in each well, wherein each well of the plurality of wells of the microplate comprises a different plurality of reactants and a solvent / s for the first reaction; optionally performing a second (R-II), third (R-III) and / or more reaction step in the wells; without purification of the reaction mixture, individually performing a biological assay with the contents of each well of the plurality of wells of the microplate; and assessing whether or not at least one compound in each well has a biological effect.

The application discloses application of Biochanin A in preparation of a medicine for inhibiting or improving uterine decidua stromal cell senescence, and belongs to the field of biomedical technology. Through drug chemical library screening and experimental verification, it is found that Biochanin A can significantly alleviate the senescence phenotype of human uterine decidua stromal cells, reduce the expression of senescence-related markers, and alleviate decidua stromal cell senescence through regulation of mitochondrial energy metabolism. The application provides a brand-new drug target and intervention strategy for improving endometrial decidua function and treating related reproductive health problems in clinic, and provides application of Biochanin A in preparation of a medicine for alleviating uterine decidua stromal cell senescence.

This invention belongs to the field of compound technology, specifically a small molecule compound inhibitor antagonizing GREM1 and its application in the treatment of colorectal cancer. The specific preparation process of this small molecule compound inhibitor is as follows: First, the DEL (DNA-encoded chemical library) library is dried under vacuum, then dissolved in a protein solution containing 5-10 µM human Grem1 protein, and incubated in a DEL screening buffer system for 30 minutes; after incubation, the Grem1 protein is immobilized using 20 µl of HisPur™ Ni-NTA Magnetic Beads, and incubation continues for 15 minutes, followed by washing with 10 × 100 µl of screening buffer under shaking, and finally removing unbound library molecules by vacuum aspiration; the small molecule inhibitor screened by this application using DNA-encoded libraries (DEL) has the advantages of small size, strong permeability, tunable pharmacokinetics, and low cost, and can inhibit the migration and invasion of colorectal cancer.