9 results about "Protein regulation" patented technology

The invention discloses a heterocyclic compound, a pharmaceutical composition and application thereof. Provided is a heterocyclic compound, or a pharmaceutically acceptable salt thereof, a stereoisomer thereof, a tautomer thereof, a polymorph thereof, a solvate thereof, an isotope derivative thereof, or a prodrug thereof; the compound has a structure as shown in a formula (I). . The heterocyclic compound provided by the invention has inhibitory activity on TEAD protein, can effectively inhibit interaction of TEAD and YAP, and further can treat diseases related to abnormal TEAD protein regulation or abnormal Hippo pathway.

The invention belongs to the technical field of respiratory disease targeted therapy and protein regulation and control, and relates to application of an ACTN2 protein enhancer in preparation of a medicine for preventing, relieving or treating asthma. The key'switch 'effect of the cytoskeleton protein ACTN2 in airway smooth muscle cell (ASMC) phenotype transformation is discovered and confirmed for the first time. The prior art mainly focuses on inflammatory pathways or bronchial relaxation, while the invention reveals that the expression down-regulation of ACTN2 is a key driving factor which causes the conversion of ASC from a resting systolic phenotype to a pathological synthetic phenotype. The discovery provides a brand new therapeutic target for asthma, especially refractory asthma accompanied by severe airway remodeling and hormone resistance.

The invention relates to the field of plant genetic engineering, in particular to an MMR1 protein for regulating and controlling ROS homeostasis of a plant root tip meristematic region as well as a coding gene and application of the MMR1 protein. The method comprises the following steps: knocking out an MMR1 gene to obtain stable transgenic lines mmr1-1 and mmr1-2 of arabidopsis thaliana, and constructing an MMR1 gene complement line and an MMRI gene overexpression line; compared with a wild type, the mutants mmr1-1 and mmr1-2 show that the root length is shortened, the root tip meristematic region is shortened, and the root tip division capability is weakened; the back-complemented strain shows that the MMR1 protein is positioned in mitochondria; the root of an overexpressed strain is longer than that of a wild type, which shows that the MMR1 gene plays an important role in the root elongation of arabidopsis thaliana, the development of a root tip meristematic zone and the cell viability of the root tip meristematic zone. According to the invention, the blank in the field of mitochondrial localization protein regulation and control of root development and ROS homeostasis is filled up, and a new genetic gene resource is provided for root improvement and transgenic breeding.

This invention relates to the field of plant genetic engineering, specifically to the MMR1 protein, its encoding gene, and its applications in regulating ROS homeostasis in the root tip meristem of plants. This invention involves knocking out the MMR1 gene to obtain stable transgenic lines of Arabidopsis thaliana. mmr1-1, mmr1-2 and build MMR1 Gene replacement lines and MMRI Gene overexpression lines; mutants compared to wild type mmr1-1, mmr1-2 The results showed shortened root length, shortened root tip meristem, and reduced root tip division ability; reintroduced lines indicated that the MMR1 protein was located in the mitochondria; the roots of the overexpressing lines were longer than those of the wild type, indicating... MMR1 The gene plays an important role in root elongation, root apical meristem development, and root apical meristem cell activity in Arabidopsis thaliana. This invention fills a gap in the field of mitochondrial-localized protein regulation of root development and ROS homeostasis, providing new genetic resources for root improvement and transgenic breeding.

The invention belongs to the technical field of plant inheritance and genetic engineering breeding, and particularly relates to application of a soybean GmLEA1-GmPM30-GmLEC1 interaction module in salt tolerance regulation and control and molecular breeding. According to the application of the soybean GmPM30 protein regulation and control network in the aspect of regulating and controlling the salt tolerance, the soybean GmPM30 protein is utilized to interact with GmLEA1 to form a heterodimer and GmLEC1 to interact with the heterodimer to form a module, and the salt tolerance is improved through a synergistic protection mechanism. Meanwhile, the invention provides a breeding method of the soybean polygene polymerization germplasm based on molecular marker-assisted selection of haplotype conjoint analysis, the polygene polymerization soybean salt-tolerant germplasm can be obtained in a short time by adopting the breeding method, the breeding efficiency is improved, and therefore the method has good practical application value.

This invention discloses a triazole compound, its preparation method, and its applications. The triazole compound is of formula I. The triazole compound provided by this invention exhibits strong binding activity to TEAD protein. After binding to TEAD protein, the compound significantly enhances the protein's thermal stability while inhibiting TEAD protein activity. It effectively disrupts the TEAD-YAP interaction and demonstrates strong anti-proliferative activity against the NCI-H226 cell line with abnormal Hippo pathway NF2 deficiency, thereby enabling the treatment of diseases related to abnormal TEAD protein regulation or Hippo pathway abnormalities.

The invention discloses a 2-pyrrolidone tolerance target mining method based on proteomics and application of the 2-pyrrolidone tolerance target mining method, and belongs to the technical field of genetic engineering. According to the invention, based on a high-resolution proteome map, a system response network of toxicity stress is analyzed, and a multi-protein regulation node considering membrane steady state, redox steady state and pathway enzyme activity is more clearly locked; a protein combination is designed in a calculation-experiment closed loop mode, terminal tolerance improvement of yield and synchronous amplification of the yield can be achieved without sacrificing growth and substrate utilization rate, the influence of genes Ncg10464, Ncg11062, Ncg10453, Ncg10983, cg2886, cg0095, cg0344 and cg0507 on the tolerance of corynebacterium glutamicum to 2-pyrrolidone is excavated, the genes are used as a modification target, a gene engineering bacterium is constructed, and the gene engineering bacterium is applied to the field of gene engineering. The yield of 2-pyrrolidone can be obviously improved, and the highest yield can reach 20.69 g / L.

The invention provides a method for multi-omics exploration of a regulation mechanism of duckweed protein content under environmental stress, and belongs to the technical field of protein regulation, target duckweed is firstly pretreated, and then corresponding environmental stress treatment is performed on the target duckweed after pretreatment, and the method is characterized in that crude protein content is measured on the target duckweed after the environmental stress treatment, and the content of protein in the target duckweed is measured; the method comprises the following steps: selecting a treatment group and a control group with significant phenotype difference according to a crude protein content determination result, carrying out transcriptome sequencing and proteome analysis, carrying out bioinformatics analysis on data of transcriptome sequencing and proteome analysis, and obtaining a regulation mechanism; the method can comprehensively, deeply and accurately reveal the internal molecular regulation mechanism of the duckweed responding to environmental stress and accumulating protein, so that a more accurate and suitable culture strategy can be obtained according to the regulation mechanism, and the protein content of the duckweed can be greatly increased.