30results about How to "Polymorphic" patented technology

The invention discloses a molecular marking method of an indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer. The developed and designed gene Bph27 is closely interlocked with molecular marking primers B471 and B58, and differential fragments can be amplified from an anti-brown planthopper donor Balamawee and a susceptible indica rice variety; the anti-brown planthopper host gene Bph27 is genetically transferred through molecular marking of a gene Bph27 locus, the required fragments can be accurately and quickly guided, the selection efficiency of the gene Bph27 is greatly improved, and a new anti-brown planthopper indica rice variety 991RB is developed.

The invention provides primers for detecting SSR (simple sequence repeat) marks of prunus pseudocerasus. The group of primers comprises nine primer pairs which can be used simultaneously in the detection process, wherein the sequences of the nine primer pairs are shown in SEQ ID NO:1 to SEQ ID NO:18. The nine SSR marks screened out have polymorphism in species or varieties of prunus pseudocerasus, genetic diversity analysis performed in 22 species or varieties of prunus pseudocerasus basically coincides with classification common sense of cerasus plants, the SSR marks are new marks existing stably, and the nine marks provided in the invention can be directly applied to more prunus pseudocerasus or even transferred to prunus avium for genetic diversity analysis of germplasm resources, construction of genetic maps and molecular mark assisted breeding.

The invention provides an SNP molecular marker and method for identifying mycobacteria, a primer composition, a kit and application, and relates to the technical field of strain identification. The SNP molecular marker comprises seventeen SNP loca distributed in an Hsp65 gene, an rrs gene and a pncA gene, and when marked in mycobacterium tuberculosis, mycobacterium bovis, Africa mycobacterium, mycobacterium avium, intracellular mycobacterium, mycobacterium fortuitum, mycobacterium kansasii, mycobacterium gastri, mycobacterium marinum,volemycobacterium, mycobacterium scrofulaceum, mycobacteriumsmegmatis, suga mycobacterium, mycobacterium abscessus, mycobacterium ulcerans, toad mycobacterium, mycobacterium gordonae and ape mycobacterium, the SNP molecular marker has polymorphism. The primercomposition can amplify the Hsp65 gene, therrs gene and thepncA gene to be used for analyzing of the SNP molecular marker and strain identification.

The invention provides a detection method for rapidly and accurately identifying ericaceae vaccinium. Following primer pairs are used for detection. Nucleotide sequences of the primer pairs are shownas follows: the nucleotide sequences of VcSSR11-F and VcSSR11-R are shown in SEQ ID NO:1 and SEQ ID NO:2; the nucleotide sequences of VcSSR14-F and VcSSR14-R are shown in SEQ ID NO:3 and SEQ ID NO:4;the nucleotide sequences of VcSSR19-F and VcSSR19-R are shown in SEQ ID NO:5 and SEQ ID NO:6; the nucleotide sequences of VcSSR28-F and VcSSR28-R are shown in SEQ ID NO:7 and SEQ ID NO:8; the nucleotide sequences of VcSSR33-F and VcSSR33-R are shown in SEQ ID NO:9 and SEQ ID NO:10. In addition, the invention provides an establishing method of allele image patterns of the ericaceae vaccinium. By reference to the method, the allele image patterns are expanded, and the number of the available allele image patterns is increased.

The invention discloses a method for developing a bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing. The method comprises the following steps: (1) acquiringtranscriptome data; (2) identifying an SSR primer; and (3) designing the SSR primer. 28 pairs of SSR primers of the invention are obtained by screening 6 species of bidens bipinnata and 9 different geographical region populations in the bidens bipinnata species, the primer has polymorphisms in 15 bidens bipinnata species, the 28 primer pairs provided by the invention can be directly applied to more species of the bidens bipinnata to analyze germplasm resources and genetic diversity, and a good foundation can be set for the molecular marker assistant breeding of the bidens bipinnata.

The invention discloses microsatellite molecular marker sites of big pacific salmons as well as polymorphic primers and application thereof. The invention discloses microsatellite molecular marker sites of big pacific salmons as well as polymorphic primers and application thereof. According to the invention, 10 microsatellite loci are screened from genomic DNA of big pacific salmons; and specificprimers are designed on flanking sequences at both ends of the microsatellite loci according to the 10 screened microsatellite loci. Polymorphism and stability are shown by amplification results usingthe primers, so that the microsatellite loci are suitable for the fields of genetic relationship identification, genetic diversity detection of population and molecule-assisted breeding of the big pacific salmons.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

The invention provides an SNP molecular marker for identifying a mycobacterium tuberculosis complex, a PCR primer and application, and relates to the technical field of molecular markers and application thereof. The SNP molecular marker comprises SNP 1 to SNP 8 which are positioned on an Hsp65 gene and SNP 9 to SNP 15 which are positioned on an rpsA gene. The SNP molecular marker has polymorphism in 10 types of mycobacteria, and the types of the mycobacteria to be detected can be identified by detecting the basic group types of the mycobacteria on the SNP sites.

The invention discloses a polymorphic high-stability resistive random access memory of an all-inorganic perovskite thin film and a preparation method of the polymorphic high-stability resistive randomaccess memory. The preparation method comprises the following steps: preparing CsPbBr3 powder by utilizing a solid-phase reaction method, and dissolving the powder into a solvent to obtain a uniformsolution; selecting a proper substrate, depositing a layer of metal film on the surface of the substrate as a lower electrode material, performing spin-coating of the prepared solution on the substrate deposited with the lower metal electrode, and performing annealing in an inert gas environment (such as nitrogen or argon) to obtain a CsPbBr3 film resistive layer; and finally, depositing a metal electrode on the CsPbBr3 thin film by using a mask plate to serve as an upper metal electrode, thereby obtaining the resistive random access memory with high stability. By regulating and controlling the saturation current, the prepared resistive random access memory has a polymorphic characteristic. The resistive random access memory with the polymorphic and high-stability characteristics has the advantages of simple structure, economy, low consumption, excellent performance and good stability.

The invention discloses a space traveling wave tube reliability evaluation method based on multi-source data fusion. The method comprises the following steps: S1, processing acquired data and extracting features; S2, calculating an edge probability of a bottom layer input node of an established Bayesian network model according to a result obtained in the step S1; S3, calculating edge probabilitiesof input nodes of other layers of the Bayesian network model according to the edge probabilities of the bottom layer input nodes obtained in the step S2; and S4, calculating a reliability point estimation result of the traveling wave tube according to the calculation result obtained in the step S3 and the Bayesian network model of the traveling wave tube. According to the invention, while staticreliability evaluation is carried out on the product, real-time on-orbit telemetry data can also be utilized to realize operation reliability evaluation on the traveling wave tube, and the result canbe used as an important basis for the working state and on-orbit health management of a satellite key single machine.

The invention discloses a kit for detecting a specific microsatellite locus of a sheep Y chromosome and an application of the kit. The kit comprises 0.6 microliter of to-be-detected sample template DNA, 0.2 microliter of specific microsatellite forward primer, 0.2 microliter of reverse primer, 1 microliter of nucleic acid amplification system 6*Buffer, 0.8 microliter of dNTP, 0.2 microliter of TaqDNA polymerase, and finally adding ddH2O to obtain 10 microliters of mixture. A microsatellite locus is screened out from a specific region of the sheep Y chromosome, and a product can be amplified when the locus is applied to male sheep, but no product is amplified when the locus is applied to a female sheet body. The male sheep group has certain polymorphism by further research, which shows that the locus can be subjected to early embryo sex identification of the sheep, and can also be applied to paternal origin evolution research of the sheep.

The invention discloses disease-resistant Toll9 protein of prawns. The disease-resistant Toll9 protein has the amino acid sequence shown in SEQ ID NO.2. The invention further discloses cDNA for encoding the disease-resistant Toll9 protein of the prawns. The cDNA has the nucleotide sequence shown in SEQ ID NO.1. The invention further discloses an expression vector containing the cDNA and provides an antibody specifically bound with the disease-resistant Toll9 protein of the prawns. Besides, the invention further provides an application of the disease-resistant Toll9 protein of the prawns and an application of the cDNA for encoding the disease-resistant Toll9 protein of the prawns.

The invention discloses a dynamic correction method and system for a game. The dynamic correction method for the game comprises the implementation steps that according to a system design of the game,basic data is configured, data couplers are arranged, correctors conduct data adjustment for the dependency couplers, dynamic correctors which mainly depend on an economic model and a mathematical model are arranged, and real user expectations and deviations are established to achieve dynamic balance; user data is collected during a game test, and the system dynamically generates self-adaptive parameters based on game data. The dynamic correction method and system for the game have the advantages that planned data is only basic data or is called reference data, and the dynamic correction system carries out correction based on the reference data; a numerical system has polymorphism, and the dynamic correction system dynamically adjusts the data according to the level of a player to reach the expectations of the player for the game; the numerical system has fault tolerance, for a data system design failure of a single module, the dynamic correction system can carry out correction according to threshold values, and therefore the avalanche effect is mitigated.

The invention discloses an SSR marker primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis of which the nucleotide sequence is shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the coeno-species seed purity of the Jinyan brilliant green No.1 brassica chinensis. The specific primer and method can complete the seed purity identificationof Jinyan brilliant green No.1 coeno-species in a laboratory for 3-5 hours, have the advantages of being rapid and stable and not being affected by the environment, can replace a traditional field seed purity identification method, and are conductive to the efficient and accurate quality control of the Jinyan brilliant green No.1 seeds.

The invention discloses an InDel molecular marker specific primer for detecting the purity of sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds. The primer is a nucleotide sequence shown in SEQ ID NO:1-2. The invention further discloses a method for identifying the purity of the sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds through the specific primer. According to thespecific primer and the method, the purity of the brassica rapa L. ssp. chinensis makino sujun 316 hybrid seeds can be identified in a laboratory within 3-5 hours, and the method has the advantages that the method is quick to use and stable and is not influenced by the environment. The method can replace a traditional field seed purity identification method and is beneficial to efficient and accurate qualify control over the sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds.

The invention discloses a method for acquiring molecular markers associated with peony-type properties and special primers thereof. The special primers for acquiring molecular markers associated with peony-type properties provided by the invention comprise the following primer pair: DNA sequences represented by the sequences 1 and 2 in the sequence table. The method provided by the invention adopts the special primers to perform PCR amplification on the peony genomes to be detected and modified polyacrylamide gel electrophoresis on the PCR amplified products, and determine the molecular markers associated with properties of the peony to be detected according to the type of electrophoretic band. The molecular marker provided by the invention is capable of detecting the peony-type properties, improving the accuracy and efficiency of the peony selective breeding and reducing the breeding period, thus providing an effective method for the breeding of peonies upon types.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

The invention relates to a data visual display method and device, equipment and a storage medium. The method can comprise the steps: obtaining a data display instruction which comprises information of a to-be-displayed object; according to the data display instruction, obtaining hierarchical data of the to-be-displayed object; and according to the hierarchical data, generating a hierarchical display graph of the to-be-displayed object and displaying the hierarchical display graph. In the process of visually displaying the to-be-displayed object, the computer equipment can obtain the data of the different hierarchies of the to-be-displayed object, and the hierarchical display graph can be generated based on the data of the different hierarchies of the to-be-displayed object, so that hierarchical display of the to-be-displayed object is realized through the hierarchical display graph. Compared with a single-level display mode in the traditional technology, the technical scheme provided by the invention enables the data display mode to be richer and more polymorphic, so that the intelligence of the data display mode is improved.

The invention discloses a high-throughput plant organ development related SSR molecular marker method of the molecular biology field. According to the method provided by the invention, with the flowers of rose as the example, more than hundred thousand of SSR primers can be obtained at one time, and 3387 pairs of the SSR primers are related to functional genes expressed in a rose development process. A lot of SSR molecular markers are selected, have polymorphism, are suitable for different application selections, can well provide genetic background for analysis and excavation of related genes, understand the domestication history of Chinese old rose, cultivation domestication of rose, and rose germplasm resource diversity, and also can provide a set of SSR molecular marker system for relative relationship identification and genetic map construction of other rosaceae plants. The method provided by the invention almost completely relies on computer simulation and calculation, gets rid of the condition restriction of laboratories and the influence of uncontrollable factors, and the obtained molecular markers are large in quantity, reliable, and have good polymorphism.

The present invention discloses a method for developing SSR primers for P. spp. plants based on transcriptome sequencing, comprising the following steps: (1) obtaining transcriptome data; (2) identifying SSR primers; (3) designing SSR primers; 28 pairs of SSR primers were obtained by screening populations from 6 species of P. genus and 9 different geographic regions in P. genus. All of the 15 P. sp. species had polymorphisms, and the present invention can be directly applied The provided 28 primer pairs were applied to more varieties of P. spp. for germplasm resources and genetic diversity analysis, which laid a good foundation for molecular marker-assisted breeding of P. spp.

The invention discloses a multi-input parallel multiplication, addition, storage and calculation integrated circuit based on a Hall bar. The multi-input parallel multiplication, addition, storage and calculation integrated circuit comprises an input coding module, a parallel multiplication module and an analog addition module. The input coding module is used for coding an input signal into a current signal. The parallel multiplication module comprises a plurality of parallel Hall strips, the current of the input end of each Hall strip is used as a multiplier of multiplication, the resistance value is used as a multiplicand, and the Hall voltage of the output end is used as a multiplication result. And the parallel Hall strips output respective multiplication calculation results, and accumulation is completed in the analog addition module, so that multiplication and addition operation of the circuit is realized. Because the multiplicand Hall resistance value is directly stored in the Hall strip, the circuit can achieve the purpose of integrating storage and calculation without an additional storage unit. The storage and calculation integrated circuit has the advantages of non-volatile storage of multiplicands, continuous change, multi-input parallel operation and the like, and is very suitable for hardware acceleration of convolutional neural network calculation.

The invention discloses an SSR marker primer for identifying the purity of Jinyan Kuailu No. 1 pakchoi seeds, which is the nucleotide sequence shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage hybrid seeds by using SSR marker primers. The specific primer and method provided by the present invention can complete the identification of the seed purity of Jinyan Kuailu No. 1 hybrid in 3-5 hours in the laboratory, has the advantages of rapidity, stability, and is not affected by the environment, etc., and can replace the traditional field seed purity The identification method is beneficial to the efficient and accurate quality control of the seeds of Jinyan Kuailu 1.

The present invention relates belongs to marine engineering field. The dispersed self-organized underwater job platform consists of several motion modules connected together with or without carrying modules. Two motion modules may be connected in one of two connection modes, with parallel rotation shafts or perpendicular rotation shafts; two carrying modules may have parallel or perpendicular geometric symmetrical axles; the rotating shaft of one motion module may be parallel or perpendicular to the geometric symmetrical axle of one carrying module; and several motion modules and / or several carrying modules may be connected to form platform of various structural forms. The platform of the present invention in dispersed structure has polymorphism and thus powerful adaptability, and is suitable for detection job in complicated and unknown underwater environment.

A method for evaluating the reliability of space traveling wave tubes based on multi-source data fusion, the method includes the following steps: S1, processing the collected data and extracting features; S2, according to the results obtained in step S1, calculating the established The marginal probability of the bottom input node of the Yasian network model; S3, according to the marginal probability of the bottom input node obtained in step S2, calculate the marginal probability of the input node of other layers of the Bayesian network model; S4, the calculation result obtained according to step S3 and The Bayesian network model of the traveling wave tube calculates the reliability point estimation results of the traveling wave tube. The present invention can also use the real-time on-orbit telemetry data to realize the operation reliability evaluation of the traveling wave tube while evaluating the static reliability of the product. in accordance with.