30results about How to "Polymorphic" patented technology

The invention provides primers for detecting SSR (simple sequence repeat) marks of prunus pseudocerasus. The group of primers comprises nine primer pairs which can be used simultaneously in the detection process, wherein the sequences of the nine primer pairs are shown in SEQ ID NO:1 to SEQ ID NO:18. The nine SSR marks screened out have polymorphism in species or varieties of prunus pseudocerasus, genetic diversity analysis performed in 22 species or varieties of prunus pseudocerasus basically coincides with classification common sense of cerasus plants, the SSR marks are new marks existing stably, and the nine marks provided in the invention can be directly applied to more prunus pseudocerasus or even transferred to prunus avium for genetic diversity analysis of germplasm resources, construction of genetic maps and molecular mark assisted breeding.

The present invention proposes a detection method for quickly and accurately identifying plants of the genus Vaccinium, using the following primer pair for detection, and the nucleotide sequences of the primer pair are as follows: the nucleotide sequences of VcSSR11‑F and VcSSR11‑R are respectively shown in SEQ ID Shown in NO:1,2; The nucleotide sequence of VcSSR14-F and VcSSR14-R is shown in SEQ ID NO:3,4 respectively; The nucleotide sequence of VcSSR19-F and VcSSR19-R is shown in SEQ ID NO: 5 and 6; the nucleotide sequences of VcSSR28‑F and VcSSR28‑R are shown in SEQ ID NO:7 and 8 respectively; the nucleotide sequences of VcSSR33‑F and VcSSR33‑R are respectively shown in SEQ ID NO:9, 10 shown. In addition, the present invention also proposes a method for constructing an allele map of Vaccinium plants. Refer to this method to expand the allelic map to enrich the number of available allelic maps.

The invention provides a SNP molecular marker, PCR primer and application for identifying Mycobacterium tuberculosis complex, and relates to the technical field of molecular marker and its application. The SNP molecular markers include SNP1-SNP 8 located on the Hsp65 gene and SNP 9-SNP 15 located on the rpsA gene. The SNP molecular marker provided by the present invention has polymorphisms in 10 kinds of mycobacteria, and the type of the mycobacteria to be detected can be identified by detecting the base type of the mycobacteria on the SNP site.

The present invention discloses an InDel molecular marker specific primer for detecting the purity of Sujun 316 non-heading Chinese cabbage hybrid seeds, which is the nucleotide sequence shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the purity of Sujun 316 non-heading Chinese cabbage hybrid seeds by using specific primers. The specific primers and method provided by the present invention can complete the identification of the purity of non-heading Chinese cabbage Sujun 316 hybrid seeds in 3-5 hours in the laboratory. The identification method is beneficial to the efficient and accurate quality control of the hybrid seeds of Sujun 316 non-heading Chinese cabbage.

The invention discloses a method for acquiring molecular markers associated with peony-type properties and special primers thereof. The special primers for acquiring molecular markers associated with peony-type properties provided by the invention comprise the following primer pair: DNA sequences represented by the sequences 1 and 2 in the sequence table. The method provided by the invention adopts the special primers to perform PCR amplification on the peony genomes to be detected and modified polyacrylamide gel electrophoresis on the PCR amplified products, and determine the molecular markers associated with properties of the peony to be detected according to the type of electrophoretic band. The molecular marker provided by the invention is capable of detecting the peony-type properties, improving the accuracy and efficiency of the peony selective breeding and reducing the breeding period, thus providing an effective method for the breeding of peonies upon types.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

The invention discloses a high-throughput plant organ development related SSR molecular marker method of the molecular biology field. According to the method provided by the invention, with the flowers of rose as the example, more than hundred thousand of SSR primers can be obtained at one time, and 3387 pairs of the SSR primers are related to functional genes expressed in a rose development process. A lot of SSR molecular markers are selected, have polymorphism, are suitable for different application selections, can well provide genetic background for analysis and excavation of related genes, understand the domestication history of Chinese old rose, cultivation domestication of rose, and rose germplasm resource diversity, and also can provide a set of SSR molecular marker system for relative relationship identification and genetic map construction of other rosaceae plants. The method provided by the invention almost completely relies on computer simulation and calculation, gets rid of the condition restriction of laboratories and the influence of uncontrollable factors, and the obtained molecular markers are large in quantity, reliable, and have good polymorphism.

The invention provides a SNP molecular marker, a method, a primer composition, a kit and an application for identifying mycobacteria, and relates to the technical field of bacterial species identification. The SNP molecular marker contains a total of 17 SNP sites, which are distributed in the Hsp65 gene, rrs gene and pncA gene. Mycobacterium endogenous, Mycobacterium fortuitum, Mycobacterium kansasii, Mycobacterium gastric, Mycobacterium marinum, Mycobacterium microti, Mycobacterium scrofula, Mycobacterium smegmatis, Mycobacterium suja, Abscessus Polymorphisms in Mycobacterium, Mycobacterium ulcerans, Mycobacterium xenopus, Mycobacterium gordonii and Mycobacterium simiensis. The above primer composition can amplify Hsp65 gene, rrs gene and pncA gene, so as to be used for subsequent analysis of SNP molecular markers and identification of strains.

The present invention discloses a method for developing SSR primers for P. spp. plants based on transcriptome sequencing, comprising the following steps: (1) obtaining transcriptome data; (2) identifying SSR primers; (3) designing SSR primers; 28 pairs of SSR primers were obtained by screening populations from 6 species of P. genus and 9 different geographic regions in P. genus. All of the 15 P. sp. species had polymorphisms, and the present invention can be directly applied The provided 28 primer pairs were applied to more varieties of P. spp. for germplasm resources and genetic diversity analysis, which laid a good foundation for molecular marker-assisted breeding of P. spp.

The invention discloses an SSR marker primer for identifying the purity of Jinyan Kuailu No. 1 pakchoi seeds, which is the nucleotide sequence shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage hybrid seeds by using SSR marker primers. The specific primer and method provided by the present invention can complete the identification of the seed purity of Jinyan Kuailu No. 1 hybrid in 3-5 hours in the laboratory, has the advantages of rapidity, stability, and is not affected by the environment, etc., and can replace the traditional field seed purity The identification method is beneficial to the efficient and accurate quality control of the seeds of Jinyan Kuailu 1.

The present invention relates belongs to marine engineering field. The dispersed self-organized underwater job platform consists of several motion modules connected together with or without carrying modules. Two motion modules may be connected in one of two connection modes, with parallel rotation shafts or perpendicular rotation shafts; two carrying modules may have parallel or perpendicular geometric symmetrical axles; the rotating shaft of one motion module may be parallel or perpendicular to the geometric symmetrical axle of one carrying module; and several motion modules and / or several carrying modules may be connected to form platform of various structural forms. The platform of the present invention in dispersed structure has polymorphism and thus powerful adaptability, and is suitable for detection job in complicated and unknown underwater environment.

A method for evaluating the reliability of space traveling wave tubes based on multi-source data fusion, the method includes the following steps: S1, processing the collected data and extracting features; S2, according to the results obtained in step S1, calculating the established The marginal probability of the bottom input node of the Yasian network model; S3, according to the marginal probability of the bottom input node obtained in step S2, calculate the marginal probability of the input node of other layers of the Bayesian network model; S4, the calculation result obtained according to step S3 and The Bayesian network model of the traveling wave tube calculates the reliability point estimation results of the traveling wave tube. The present invention can also use the real-time on-orbit telemetry data to realize the operation reliability evaluation of the traveling wave tube while evaluating the static reliability of the product. in accordance with.

The invention discloses disease-resistant Toll9 protein of prawns. The disease-resistant Toll9 protein has the amino acid sequence shown in SEQ ID NO.2. The invention further discloses cDNA for encoding the disease-resistant Toll9 protein of the prawns. The cDNA has the nucleotide sequence shown in SEQ ID NO.1. The invention further discloses an expression vector containing the cDNA and provides an antibody specifically bound with the disease-resistant Toll9 protein of the prawns. Besides, the invention further provides an application of the disease-resistant Toll9 protein of the prawns and an application of the cDNA for encoding the disease-resistant Toll9 protein of the prawns.