30results about How to "Polymorphic" patented technology

The invention discloses a molecular marking method of an indica rice variety subjected to anti-brown planthopper host gene Bph27 transfer. The developed and designed gene Bph27 is closely interlocked with molecular marking primers B471 and B58, and differential fragments can be amplified from an anti-brown planthopper donor Balamawee and a susceptible indica rice variety; the anti-brown planthopper host gene Bph27 is genetically transferred through molecular marking of a gene Bph27 locus, the required fragments can be accurately and quickly guided, the selection efficiency of the gene Bph27 is greatly improved, and a new anti-brown planthopper indica rice variety 991RB is developed.

The invention provides an SNP molecular marker and method for identifying mycobacteria, a primer composition, a kit and application, and relates to the technical field of strain identification. The SNP molecular marker comprises seventeen SNP loca distributed in an Hsp65 gene, an rrs gene and a pncA gene, and when marked in mycobacterium tuberculosis, mycobacterium bovis, Africa mycobacterium, mycobacterium avium, intracellular mycobacterium, mycobacterium fortuitum, mycobacterium kansasii, mycobacterium gastri, mycobacterium marinum,volemycobacterium, mycobacterium scrofulaceum, mycobacteriumsmegmatis, suga mycobacterium, mycobacterium abscessus, mycobacterium ulcerans, toad mycobacterium, mycobacterium gordonae and ape mycobacterium, the SNP molecular marker has polymorphism. The primercomposition can amplify the Hsp65 gene, therrs gene and thepncA gene to be used for analyzing of the SNP molecular marker and strain identification.

The invention provides a detection method for rapidly and accurately identifying ericaceae vaccinium. Following primer pairs are used for detection. Nucleotide sequences of the primer pairs are shownas follows: the nucleotide sequences of VcSSR11-F and VcSSR11-R are shown in SEQ ID NO:1 and SEQ ID NO:2; the nucleotide sequences of VcSSR14-F and VcSSR14-R are shown in SEQ ID NO:3 and SEQ ID NO:4;the nucleotide sequences of VcSSR19-F and VcSSR19-R are shown in SEQ ID NO:5 and SEQ ID NO:6; the nucleotide sequences of VcSSR28-F and VcSSR28-R are shown in SEQ ID NO:7 and SEQ ID NO:8; the nucleotide sequences of VcSSR33-F and VcSSR33-R are shown in SEQ ID NO:9 and SEQ ID NO:10. In addition, the invention provides an establishing method of allele image patterns of the ericaceae vaccinium. By reference to the method, the allele image patterns are expanded, and the number of the available allele image patterns is increased.

The invention discloses a method for developing a bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing. The method comprises the following steps: (1) acquiringtranscriptome data; (2) identifying an SSR primer; and (3) designing the SSR primer. 28 pairs of SSR primers of the invention are obtained by screening 6 species of bidens bipinnata and 9 different geographical region populations in the bidens bipinnata species, the primer has polymorphisms in 15 bidens bipinnata species, the 28 primer pairs provided by the invention can be directly applied to more species of the bidens bipinnata to analyze germplasm resources and genetic diversity, and a good foundation can be set for the molecular marker assistant breeding of the bidens bipinnata.

The invention discloses microsatellite molecular marker sites of big pacific salmons as well as polymorphic primers and application thereof. The invention discloses microsatellite molecular marker sites of big pacific salmons as well as polymorphic primers and application thereof. According to the invention, 10 microsatellite loci are screened from genomic DNA of big pacific salmons; and specificprimers are designed on flanking sequences at both ends of the microsatellite loci according to the 10 screened microsatellite loci. Polymorphism and stability are shown by amplification results usingthe primers, so that the microsatellite loci are suitable for the fields of genetic relationship identification, genetic diversity detection of population and molecule-assisted breeding of the big pacific salmons.

The invention provides an SNP molecular marker for identifying a mycobacterium tuberculosis complex, a PCR primer and application, and relates to the technical field of molecular markers and application thereof. The SNP molecular marker comprises SNP 1 to SNP 8 which are positioned on an Hsp65 gene and SNP 9 to SNP 15 which are positioned on an rpsA gene. The SNP molecular marker has polymorphism in 10 types of mycobacteria, and the types of the mycobacteria to be detected can be identified by detecting the basic group types of the mycobacteria on the SNP sites.

The invention discloses a kit for detecting a specific microsatellite locus of a sheep Y chromosome and an application of the kit. The kit comprises 0.6 microliter of to-be-detected sample template DNA, 0.2 microliter of specific microsatellite forward primer, 0.2 microliter of reverse primer, 1 microliter of nucleic acid amplification system 6*Buffer, 0.8 microliter of dNTP, 0.2 microliter of TaqDNA polymerase, and finally adding ddH2O to obtain 10 microliters of mixture. A microsatellite locus is screened out from a specific region of the sheep Y chromosome, and a product can be amplified when the locus is applied to male sheep, but no product is amplified when the locus is applied to a female sheet body. The male sheep group has certain polymorphism by further research, which shows that the locus can be subjected to early embryo sex identification of the sheep, and can also be applied to paternal origin evolution research of the sheep.

The invention discloses a dynamic correction method and system for a game. The dynamic correction method for the game comprises the implementation steps that according to a system design of the game,basic data is configured, data couplers are arranged, correctors conduct data adjustment for the dependency couplers, dynamic correctors which mainly depend on an economic model and a mathematical model are arranged, and real user expectations and deviations are established to achieve dynamic balance; user data is collected during a game test, and the system dynamically generates self-adaptive parameters based on game data. The dynamic correction method and system for the game have the advantages that planned data is only basic data or is called reference data, and the dynamic correction system carries out correction based on the reference data; a numerical system has polymorphism, and the dynamic correction system dynamically adjusts the data according to the level of a player to reach the expectations of the player for the game; the numerical system has fault tolerance, for a data system design failure of a single module, the dynamic correction system can carry out correction according to threshold values, and therefore the avalanche effect is mitigated.

The invention discloses an SSR marker primer for identifying seed purity of Jinyan brilliant green No.1 brassica chinensis of which the nucleotide sequence is shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the coeno-species seed purity of the Jinyan brilliant green No.1 brassica chinensis. The specific primer and method can complete the seed purity identificationof Jinyan brilliant green No.1 coeno-species in a laboratory for 3-5 hours, have the advantages of being rapid and stable and not being affected by the environment, can replace a traditional field seed purity identification method, and are conductive to the efficient and accurate quality control of the Jinyan brilliant green No.1 seeds.

The invention discloses an InDel molecular marker specific primer for detecting the purity of sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds. The primer is a nucleotide sequence shown in SEQ ID NO:1-2. The invention further discloses a method for identifying the purity of the sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds through the specific primer. According to thespecific primer and the method, the purity of the brassica rapa L. ssp. chinensis makino sujun 316 hybrid seeds can be identified in a laboratory within 3-5 hours, and the method has the advantages that the method is quick to use and stable and is not influenced by the environment. The method can replace a traditional field seed purity identification method and is beneficial to efficient and accurate qualify control over the sujun 316 brassica rapa L. ssp. chinensis makino hybrid seeds.

The invention relates to a data visual display method and device, equipment and a storage medium. The method can comprise the steps: obtaining a data display instruction which comprises information of a to-be-displayed object; according to the data display instruction, obtaining hierarchical data of the to-be-displayed object; and according to the hierarchical data, generating a hierarchical display graph of the to-be-displayed object and displaying the hierarchical display graph. In the process of visually displaying the to-be-displayed object, the computer equipment can obtain the data of the different hierarchies of the to-be-displayed object, and the hierarchical display graph can be generated based on the data of the different hierarchies of the to-be-displayed object, so that hierarchical display of the to-be-displayed object is realized through the hierarchical display graph. Compared with a single-level display mode in the traditional technology, the technical scheme provided by the invention enables the data display mode to be richer and more polymorphic, so that the intelligence of the data display mode is improved.

The invention discloses a multi-input parallel multiplication, addition, storage and calculation integrated circuit based on a Hall bar. The multi-input parallel multiplication, addition, storage and calculation integrated circuit comprises an input coding module, a parallel multiplication module and an analog addition module. The input coding module is used for coding an input signal into a current signal. The parallel multiplication module comprises a plurality of parallel Hall strips, the current of the input end of each Hall strip is used as a multiplier of multiplication, the resistance value is used as a multiplicand, and the Hall voltage of the output end is used as a multiplication result. And the parallel Hall strips output respective multiplication calculation results, and accumulation is completed in the analog addition module, so that multiplication and addition operation of the circuit is realized. Because the multiplicand Hall resistance value is directly stored in the Hall strip, the circuit can achieve the purpose of integrating storage and calculation without an additional storage unit. The storage and calculation integrated circuit has the advantages of non-volatile storage of multiplicands, continuous change, multi-input parallel operation and the like, and is very suitable for hardware acceleration of convolutional neural network calculation.