A primer for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage seeds and its application

A cabbage and seed technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as heavy workload, backlog of seed inventory, and susceptibility to environmental influences, and achieve stability. Good results

Active Publication Date: 2022-07-08
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of seed purity identification uses direct observation of the characteristics and consistency of specific growth and development stages of crops in the field. There are factors such as long cycle, heavy workload and easy to be affected by the environment, which often conflict with the peak sales season of seeds, resulting in seed Overstock

Method used

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  • A primer for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage seeds and its application
  • A primer for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage seeds and its application
  • A primer for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage seeds and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Materials

[0024] Three individual plants of male parent material, female parent material, and Jinyan Kuailu No. 1 hybrid were used for primer screening. 92 seeds of the hybrid Jinyan Kuailu No. 1 produced by 6 contracted farmers in the seed production base were randomly selected for seed purity identification.

[0025] 2. Screening of SSR primers

[0026] According to the genome resequencing data of Chinese cabbage, multiple pairs of primers were designed to detect the polymorphism of Jinyan Kuailu No. 1, the female parent material and the male parent material, and the primer pair A09-17 with codominant differences was screened out. The sequence is as follows :

[0027] F: 5'-TCCTCGAGTTTCGCTTCATATCCTG-3' (SEQ ID NO:1)

[0028] R: 5'-AGTCTGGAAACTTACATGGAAGTAAAATACAAAAC-3' (SEQ ID NO:2)

[0029] The marker has good repeatability and clear bands. The primer is used as a specific primer, and the genomic DNA of the sample to be tested is used as a template for PCR a...

Embodiment 2

[0044] Comparative test:

[0045] About 100 seeds of Jinyan Kuailu No. 1 commercial variety produced by 6 contracted farmers in the seed production base were randomly selected from each household to be tested, and the fields were open-ditched and sown at a density of 200 plants per square meter. After sowing, about 3 The seedlings will emerge from the sky and grow to the stage of 7 leaves and 1 heart. According to the plant height, leaf color, leaf shape, petiole color, length, width and other performance characteristics of the samples to be tested, whether they conform to the uniformity of Jinyan Kuailu No. 1 commercial variety and consistency, determine whether it is the Jinyan Express No. 1 commodity variety, and count the seed purity of the sample to be tested.

[0046]

[0047] Conclusion: The identification of the seed purity of Jinyan Kuailu No. 1 by the SSR molecular marker primer provided by the present invention is consistent with the identification of the seed pu...

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Abstract

The invention discloses an SSR marker primer for identifying the purity of Jinyan Kuailu No. 1 pakchoi seeds, which is the nucleotide sequence shown in SEQ ID NO: 1-2. The invention further discloses a method for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage hybrid seeds by using SSR marker primers. The specific primer and method provided by the present invention can complete the identification of the seed purity of Jinyan Kuailu No. 1 hybrid in 3-5 hours in the laboratory, has the advantages of rapidity, stability, and is not affected by the environment, etc., and can replace the traditional field seed purity The identification method is beneficial to the efficient and accurate quality control of the seeds of Jinyan Kuailu 1.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an SSR marker primer for identifying the purity of Jinyan Kuailu No. 1 Chinese cabbage seeds and its application. Background technique [0002] Chinese cabbage ( Brassica rapa L. ssp. chinensis ) belongs to the Brassica genus of the cruciferous family, belonging to non-heading cabbage, which is rich in protein, carbohydrates, dietary fiber, carotene, vitamins and calcium, phosphorus, iron, etc. Compared with traditional Chinese cabbage, Chinese cabbage has a short growth cycle and can be planted and marketed almost all year round. Its fiber content is low, its texture is soft, sweet and fragrant, and it can be eaten from seedlings to adults. It is the largest cultivation area in my country. It is one of the largest and most widely distributed popular vegetables and plays an important role in ensuring the supply of vegetables in our country. [0003] High-quality varieties are the k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 王超楠张红华德平黄志银李梅范伟强张斌刘晓晖
Owner TIANJIN RES INST OF VEGETABLE
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