3 results about "Rosa arvensis" patented technology

The application discloses a Rosa chinensis disease prevention and growth promotion bacterium LX6 strain and application thereof, and belongs to the field of microorganism strains and application thereof. The Rosa chinensis disease prevention and growth promotion bacterium LX6 is preserved in the China General Microbiological Culture Collection Center on May 16, 2024, and has a preservation number of CGMCC No. 30657 and a classification name of Pseudomonas fluorescens. The bacterium strain has the ability to resist high-concentration salt ions and various heavy metal ions. The bacterium strain can inhibit the growth of a Rosa chinensis black spot pathogen, i.e., Discosia rosea, and can also antagonize the growth of plant pathogenic fungi, i.e., Colletotrichum gloeosporioides, Rhizoctonia solani, Fusarium oxysporum and Fusarium equiseti, so as to prevent and treat the Rosa chinensis black spot and powdery mildew, and promote the growth and flowering of the Rosa chinensis. The application provides a fine biocontrol strain for preventing and treating the diseases of the Rosa chinensis and promoting the growth of the Rosa chinensis, and has important significance for green prevention and control technology of the diseases of the Rosa chinensis and sustainable development of the Rosa chinensis industry.

This invention discloses a universal method for preparing nuclear suspensions suitable for flow cytometry of Rosa species, belonging to the field of plant biotechnology. The method uses WPB dissociation buffer and follows a standardized procedure to process Rosa leaves in different storage states through sample dissociation, filtration, staining, and incubation steps: leaf tissue is dissociated using WPB dissociation buffer pre-cooled to 4°C, allowed to stand on ice, filtered through a 40µm cell filter, and brought to volume. Propidium iodide and ribonuclease A are added for staining and incubation before flow cytometry analysis. This invention is directly applicable to Rosa leaves in three storage states: fresh, silica gel dried, and -80°C frozen. It is simple to operate, highly versatile, and the prepared nuclear suspension yields high-quality peak histograms with low coefficient of variation when analyzed by flow cytometry, providing an efficient and standardized technical solution for large-scale ploidy analysis and genome size determination of Rosa species.