Method for developing bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing

A technology of transcriptome sequencing and ghost needle grass, applied in the field of molecular biology, can solve the problem of limited number of SSR primers

Active Publication Date: 2018-03-23
SHENYANG NORMAL UNIV
View PDF7 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no complete genome sequence information of P. sp., and the number of SSR primers is very limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for developing bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing
  • Method for developing bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing
  • Method for developing bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0089] The SSR primer pair developed based on the transcriptome sequencing of P. figure 1 shown), SSR primer development, DNA extraction, SSR primer screening and variety identification were obtained.

[0090] In the present invention, using the EST sequence obtained by transcriptome sequencing to develop the SSR primer pair for P. sp.

[0091] 1. Transcriptome sequencing of P.

[0092] After extracting the total RNA from the leaves of P. genus plants by the Trizol method, enrich the eukaryotic mRNA with magnetic beads containing Oligo(dT); add fragmentation buffer to fragment the mRNA into short fragments, use the mRNA as a template, and use six bases Random primers (randomhexamers) synthesize the first cDNA strand, then add buffer, dNTPs, RNaseH and DNApolymeraseI to synthesize the second cDNA strand, after purification by QiaQuickPCR kit and elution with EB buffer, do end repair, add poly( A) Connect the sequencing adapter, then use agarose gel electrophoresis to select t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for developing a bidens bipinnata plant SSR (Simple Sequence Repeats) primer based on transcriptome sequencing. The method comprises the following steps: (1) acquiringtranscriptome data; (2) identifying an SSR primer; and (3) designing the SSR primer. 28 pairs of SSR primers of the invention are obtained by screening 6 species of bidens bipinnata and 9 different geographical region populations in the bidens bipinnata species, the primer has polymorphisms in 15 bidens bipinnata species, the 28 primer pairs provided by the invention can be directly applied to more species of the bidens bipinnata to analyze germplasm resources and genetic diversity, and a good foundation can be set for the molecular marker assistant breeding of the bidens bipinnata.

Description

technical field [0001] The invention relates to a method for developing SSR primers of P. sp. plants based on transcriptome sequencing, and belongs to the technical field of molecular biology. Background technique [0002] Ghost needle grass (Bidens bipinnata L.) belongs to the plant of the family Asteraceae. Annual herb with erect stems, 30-120cm high. Born in roadside wasteland, hillsides and fields, it is widely distributed all over the country, and the whole plant is used as medicine. It is an important medicinal plant. It can clear away heat, detoxify, and dissipate blood stasis. It is used to treat diseases such as dysentery, diarrhea, and hepatitis. It also has certain curative effects on cardiovascular and cerebrovascular diseases and tumors. Due to the distribution of the genus Ghost needles in both the north and the south of my country, the range of ecological adaptation is large, and there are many variations in morphology, which makes it difficult to distinguis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6811C12Q1/6806C12Q1/6895C12N15/10C40B50/06G06F19/28
CPCC12N15/1096C12Q1/6806C12Q1/6811C12Q1/6895C12Q2600/156C40B50/06G16B50/00C12Q2525/191C12Q2531/113
Inventor 逄洪波高秋李玥莹吴隆坤刘宏鑫金海涛
Owner SHENYANG NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap