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31 results about "Prunus pseudocerasus" patented technology

Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus

The invention discloses a method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus. The method is characterized in that the tissue culture seedlings of Gisela 5 and 6 in the root induction stage, which are produced in the laboratory, are adopted; on the 14th day of root induction, the shooting-up seedlings are selected and are further cultured into strong seedlings; the seedlings can be used after the seedlings become slightly strong half a month later; the matrix used for cuttage is formed by uniformly mixing pearlite, vermiculite, turfy soil and river sands in a ratio of 2:2:1:1; in the period, the temperature and the humidity in the greenhouse are respectively ensured to be 26-28 DEG C and 50-60%; the bactericide is sprayed every other day; a kung fu insecticide is sprayed 5-7 days after cuttage; the spraying times of the insecticide depends and is not more than 5; the insecticide is sprayed every 2-3 days; whether the seedlings root can be observed onthe 10th-14th day; and the plantlets can be transplanted to plugs to be acclimatized 20 days later. The experimental results show that by adopting the method, each plant can have 3 roots, the cycle is short and the effect is good; and the technology can be used for scale production of Gisela virus-free stocks.
Owner:天津樱桃谷农业科技发展有限公司

Method and special primer for simultaneously detecting cherry whether to be infected by apple chlorotic leaf spot virus and prunus necrotic ringspot virus

The invention discloses a method and a special primer for simultaneously detecting whether a cherry to be infected by an apple chlorotic leaf spot virus and a prunus necrotic ringspot virus. The primer used for detecting plant viruses comprises a primer pair A and a primer pair B, wherein the primer pair A is used for detecting the apple chlorotic leaf spot virus; a primer sequence is shown as SEQ ID No.1 (sequence identification number 1); the other primer sequence is shown as SEQ ID No.2; the primer pair B is used for detecting the prunus necrotic ringspot virus; a primer sequence is shown as SEQ ID No.3; the other primer sequence is shown as SEQ ID No.4. A multi-RT-PCR (reverse transcription-polymerase chain reaction) established in the primer can simultaneously detect ACLSV (apple chlorotic leaf spot virus) and PNRSV (prunus necrotic ringspot virus) compounded infection of the cherry in production, and saves time compared with a single RT-PCR; the operation is simpler; in addition, consumed drugs in a test are reduced; and economy is facilitated. When a total amount of RNA (ribonucleic acid) of an infected plant in a reverse transcription system ranges from 10 micrograms to 4 micrograms, the two viruses can still be detected simultaneously after PCR (polymerase chain reaction) amplification is performed.
Owner:CHINA AGRI UNIV

Special micro-organism-inhibiting and freshness-retaining paper for cerasus pseudocerasus fruits and freshness-retaining method thereof

The present invention discloses special micro-organism-inhibiting and freshness-retaining paper for cerasus pseudocerasus fruits and a freshness-retaining method thereof. The freshness-retaining paperis prepared from 350x190 mm double-layer non-woven fabric, 1% of a 1-methylcyclopropene adsorbent and 0.1% of garlic essential oil and prepared by two internal and external successive printing on thedouble-layer nonwoven fabric. The special micro-organism-inhibiting and freshness-retaining method for the cerasus pseudocerasus fruits comprises the following steps: 0.25 g of 1-methylcyclopropene medicinal powder is uniformly placed in middle of the 350x190 mm double-layer non-woven fabric; the double-layer non-woven fabric loaded with the 1-methylcyclopropene medicinal powder is subjected to edge sealing using a non-woven fabric sewing machine; and 0.1% of garlic essential oil is printed on center of the non-woven fabric in a cross-type, and the non-woven fabric printed with the garlic essential oil is quickly dried and bagged for use. Advantages are that: 1 sheet of the freshness-retaining paper can conduct seal freshness-retaining of 3-5 kilograms of the cerasus pseudocerasus fruits;and the special micro-organism-inhibiting and freshness-retaining paper is simple in formula, safe, easy to operate, low in cost, good in freshness-retaining effects, high in stability, high in broad-spectrum applicability, and easy for wide promotion and application.
Owner:GUIZHOU UNIV

Method for rapidly detecting prunus necrotic ring-spot virus of sweet cherries

The invention relates to the technical field of plant virus detection, and in particular relates to a method for detecting prunus necrotic ring-spot virus of sweet cherries. The method comprises the following steps of: (1), designing and synthesizing a DNA fragment and a probe; (2), preparing nanogold; (3), preparing a nanogold-probe 1 compound; (4), assembling a test strip; (5), preparing a sample; (6), performing exonuclease III signal amplification; and (7), detecting the test strip. According to the method in the invention, the test strip is prepared by adoption of a nucleic acid detectionprobe; an antibody is unnecessary to prepare; the preparation process is simple; the cost is low; the probe is designed for a highly specific conserved region of the prunus necrotic ring-spot virus of the sweet cherries; the detection effect is good; the specificity is high; false positive is difficultly generated; signal amplification is carried out by utilization of cyclic enzyme digestion of exonuclease III; the detection sensitivity is increased; furthermore, a professional staff and a special instrument are unnecessary; carrying and detection are convenient; a result can be measured by naked eyes; the colour development time is short; and the colour development time is only 10 min.
Owner:LUDONG UNIVERSITY

Use of an extract of part of a rocket plant for stimulating the defenses of plants and trees and associated composition and method

PendingUS20200128833A1BiocideWatering devicesBunias erucagoAlmond tree
The use of an extract of at least a part of rocket plants, preferably chosen from the group of rocket plants of the Eruca (Eruca sativa; Eruca vesicaria, etc.), Diplotaxis (Diplotaxis erucoides, Diplotaxis tenuifolia, Diplotaxis muralis, etc.), Bunias (Bunias erucago, Bunias orientalis, etc.), Erucastrum (Erucastrum nasturtiifolium, Erucastrum incanum, etc.) and Cakile genuses, stimulates the defences of plants or trees and reduces the effects of bacteria and fungi on plants and trees.
In particular, the effects:
    • of Xylella fastidiosa bacteria on myrtle-leaf milkwort, grape vines, olive trees, citrus trees, oleander, almond trees, coffee trees, peach trees and stone fruit trees, oak trees, lavender, rosemary, or broom,
    • of Xanthomonas arboricola pv. Pruni bacteria on Prunus spp., and preferably the following group of fruit/nut trees: apricot trees, almond trees, cherry trees, peach trees, plum trees, P. salicina, cherry laurel and other exotic or omamental Prunus species, including P. davidiana and P. laurocerasus,
    • of Pear Decline Phytoplasma bacteria or Candidatus Phytoplasma pyri on pear trees, and
    • of Candidatus Phytoplasma solani bacteria on grape vines, lavender, potato plants, tomato plants, aubergine plants, pepper plants and tobacco plants.
Owner:MARTINEZ BARBREAU CHRISTELLE

Tissue culture rapid propagation method of prunus cerasifera

The invention belongs to the technical field of agricultural biology and particularly relates to a tissue culture rapid propagation method of prunus cerasifera. The method comprises the following steps: prunus cerasifera with axillary bud stems is sterilized and disinfected to serve as an explant, a bud induction medium is firstly inoculated with the explant for primary culture to obtain axillarybud seedlings, then, a proliferation medium is inoculated with the axillary bud seedlings for proliferation culture, multi-cluster buds obtained by proliferation are cut an divided into 2-4 parts, a height increase medium containing GA3 is inoculated with the multi-cluster buds for height increase culture, single aseptic seedling at a height of 2 cm or above is cut off, a rooting medium is inoculated for rooting, the rest low and dense multi-cluster buds are subcultured again to the proliferation medium in a circulating manner, seedling exercise is performed after aseptic seedlings on the rooting medium come up white taproots and multiple fibrous roots, the seedlings are transplanted to a medium, and seedlings of the prunus cerasifera are obtained. The tissue culture rapid propagation method can obtain strong seedlings more easily than single proliferation culture, and the proliferation coefficient is 3 times higher than that with single height increase culture.
Owner:FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI

Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus

The invention discloses a method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus. The method is characterized in that the tissue culture seedlings of Gisela 5 and 6 in the root induction stage, which are produced in the laboratory, are adopted; on the 14th day of root induction, the shooting-up seedlings are selected and are further cultured into strong seedlings; the seedlings can be used after the seedlings become slightly strong half a month later; the matrix used for cuttage is formed by uniformly mixing pearlite, vermiculite, turfy soil and river sands in a ratio of 2:2:1:1; in the period, the temperature and the humidity in the greenhouse are respectively ensured to be 26-28 DEG C and 50-60%; the bactericide is sprayed every other day; a kung fu insecticide is sprayed 5-7 days after cuttage; the spraying times of the insecticide depends and is not more than 5; the insecticide is sprayed every 2-3 days; whether the seedlings root can be observed on the 10th-14th day; and the plantlets can be transplanted to plugs to be acclimatized 20 days later. The experimental results show that by adopting the method, each plant can have 3 roots, the cycle is short and the effect is good; and the technology can be used for scale production of Gisela virus-free stocks.
Owner:天津樱桃谷农业科技发展有限公司

A kind of method for planting cherry blossoms in plateau cities above 4,000 meters

The invention discloses a method for planting hairy cherry blossoms in a plateau city of more than 4,000 meters, comprising the following steps: S1 selecting and clearing a planting area; S2 tree digging and carrying out digging treatment; S3 planting hole preparation; S4 transplanting process, in the planting hole Use rooting water to form mud to wrap the roots, add bacteriostatic agents and water-retaining agents, and use root-fixing water to shape the roots; S5 maintenance process; the beneficial effects of the present invention are: soil microorganisms are used to remove residual branches and leaves produced by combing branches and removing leaves. As raw materials, the fermentation liquid produced after fermentation is configured as a bactericide, and the fermented residue is used as a bacteriostatic agent, which reflects the economic benefit of recycling, and at the same time increases the soil viscosity and contains prunus; , is adsorbed by the water-retaining agent, so that the roots of the trees can fully absorb water and solve the serious problem of water loss; through the water-retaining agent and the water ring weir, the substances are fully absorbed by the trees, reducing the consumption of microorganisms in the enhancer and improving the soil microorganisms. content.
Owner:CHENGDU HUANMEI GARDEN ECOLOGICAL CO LTD

A kind of southern sweet cherry improved ufo shape dwarf dense planting cultivation method

The invention relates to a stunted close planting cultivation method of prunusavium in Southern warm and wet regions. The method mainly comprises the steps of constructing garden plots and facilities,constructing a cross-shaped bracket, performing transplantation, and performing trimming and pruning of improved UFO shape tree shapes. The improved UFO shaped stunted close planting cultivation method disclosed by the invention has the characteristics that illumination and ventilation are good, trimming is simple, shaping is early, flowers are early to form, fruits are early to bear, yielding ability is high, and viewing and picking are convenient; in Southern regions, at the fourth-year full fruit period of the prunusavium, the yield of the prunusavium each mu can reach 600-650 kg, early blossoming and early fruiting characteristics that flowers are formed in two years and fruits are obtained in three years can be realized, the problems that the prunusavium is fruitless in many years, the prunusavium blossoms but not bears fruits, and diseases and insect pests are frequent to generate in production of the prunusavium in the Southern warm and wet regions are solved, and besides, thecultivation pattern is also quite suitable for tourism picking and has important significance for the development of the prunusavium industry in the Southern warm and wet regions.
Owner:台州市农业科学研究院

Staggered-layer cultivation method for prunus avium and four-layer arc-shaped shed frame used in method

ActiveCN109220283AOvercome the disadvantage of being difficult to balanceIn line with the law of natural growthGraftingCultivating equipmentsMain branchComputer science
The invention discloses a staggered-layer cultivation method for prunus avium and a four-layer arc-shaped shed frame used in the method. The staggered-layer cultivation method comprises the steps thatthe four-layer shed frame is set up in a field planting cherry orchard, odd-layer trees and even-layer trees are arranged in the same row at intervals, the main branches of the odd-layer trees grow on frame surfaces of shed frame bodies in the first layer and the third layer, the main branches of adjacent even-layer trees grow on a frame surfaces of shed frame bodies in the second layer and the fourth layer, and the odd-layer trees and the even-layer trees are alternately planted in staggered layers. The four-layer shed frame comprises upright columns 1 which are arranged vertically and horizontally, the two or more upright columns 1 are arranged into one row and fixedly buried, the upright columns 1 are each provided with four layers of arc-shaped cross arms 2, and the arc-shaped cross arms, in the same layer, on each row of upright columns 1 are connected through a pull rope into a shed frame surface. According to the cultivation method, staggered-layer cultivation is adopted, the tree structure is simpler and more convenient to manage, and production is early and stable. The four-layer arc-shaped shed frame is firm in structure and simple and flexible in building method, the management work spacer of flowers and fruits in the orchard can be controlled, and the orchard management cost is helped to be reduced.
Owner:LIANYUNGANG ACAD OF AGRI SCI
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