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Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus

A test-tube seedling and cutting technology, applied in the application, cultivation, agriculture and other directions, to achieve the effects of high work efficiency, large labor and low cost

Inactive Publication Date: 2011-08-03
天津樱桃谷农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the cutting of cherry test-tube seedlings in China

Method used

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  • Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Pre-processing of material selection:

[0044]Choose to induce rooting for 14 days, the height of the seedlings is 16 cm, and the seedlings are raised, and the seedlings are further cultivated. The light is 14 hours, the temperature is 26 ° C, and the humidity is 70%. Cutting test tube seedling material. It should be noted that the selected seedlings for induced rooting must be fully strengthened, and the seedlings should be strong, and the leaves will become larger and greener, otherwise the rooting rate will be very low.

[0045] (2) Selection and processing of cuttings:

[0046] Three hormones, indole acetic acid (IBA), ABT-1 rooting powder, and ABT-6 rooting powder were used to induce rooting, and the test tube seedlings were cut into upper and lower parts, ensuring that the length of the lower part was 3 cm. Cut out V-shaped wounds at an interval of 3-4 cm on the upper part, soak in different concentrations of hormones for 20 seconds, put the upper part into ...

Embodiment 2

[0056] (1) Pre-processing of material selection:

[0057] Choose to induce rooting for 15 days, grow seedlings with a height of 15 cm, and further cultivate strong seedlings, wherein the light is 16 hours, the temperature is 26°C, and the humidity is 60%. Cutting test tube seedling material. It should be noted that the selected seedlings for induced rooting must be fully strengthened, and the seedlings should be strong, and the leaves will become larger and greener, otherwise the rooting rate will be very low.

[0058] (2) Selection and processing of cuttings:

[0059] Three hormones, indole acetic acid (IBA), ABT-1 rooting powder, and ABT-6 rooting powder were used to induce rooting, and the test tube seedlings were cut into upper and lower parts, ensuring that the length of the lower part was 3 cm. Cut out V-shaped wounds at an interval of 4 cm on the upper part, soak in different concentrations of hormones for 20 seconds, put the upper part into a flat plate as a whole, b...

Embodiment 3

[0068] (1) Pre-processing of material selection:

[0069] Choose to induce rooting for 15 days, and the height of the seedlings is 25 cm to grow taller, and further cultivate the strong seedlings, wherein the light is 14 hours, the temperature is 26 ° C, and the humidity is 70%. Cutting test tube seedling materials;

[0070] (2) Selection and processing of cuttings:

[0071] Cut the test tube seedlings into upper and lower parts, and ensure that the length of the lower part is 3 cm. Use a scalpel to cut the upper part into a V-shaped wound at an interval of 3 cm. After soaking in different concentrations of hormones for 20 seconds, put the upper part into a flat plate as a whole In the middle, bury it with a mixed substrate, only expose the top buds, and pour water; the hormones therein are: indole acetic acid, No. ABT-1 rooting powder, and No. ABT-6 rooting powder, with a concentration of 0.4 mg / L; The test-tube plantlets are the tissue culture seedlings of Gisela No. 5 or ...

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Abstract

The invention discloses a method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus. The method is characterized in that the tissue culture seedlings of Gisela 5 and 6 in the root induction stage, which are produced in the laboratory, are adopted; on the 14th day of root induction, the shooting-up seedlings are selected and are further cultured into strong seedlings; the seedlings can be used after the seedlings become slightly strong half a month later; the matrix used for cuttage is formed by uniformly mixing pearlite, vermiculite, turfy soil and river sands in a ratio of 2:2:1:1; in the period, the temperature and the humidity in the greenhouse are respectively ensured to be 26-28 DEG C and 50-60%; the bactericide is sprayed every other day; a kung fu insecticide is sprayed 5-7 days after cuttage; the spraying times of the insecticide depends and is not more than 5; the insecticide is sprayed every 2-3 days; whether the seedlings root can be observed onthe 10th-14th day; and the plantlets can be transplanted to plugs to be acclimatized 20 days later. The experimental results show that by adopting the method, each plant can have 3 roots, the cycle is short and the effect is good; and the technology can be used for scale production of Gisela virus-free stocks.

Description

technical field [0001] The invention belongs to the technical field of plant seedling propagation, in particular to a method for rapid propagation of cherry test-tube seedlings by cutting. Background technique [0002] Although the cultivation of cherry has a long history in my country, its distribution area is relatively small. In recent years, cherry, as an early market, delicious and fresh fruit, has attracted more and more people's attention. Many areas are planning to introduce or expand the cultivation area, and there is an urgent need for a large number of seedlings in production. The speed of propagating cherries in the traditional way is slow, which is far from meeting the needs. For this reason, many domestic units have carried out test-tube propagation of cherries. [0003] Cutting rapid propagation technology of test-tube seedlings is a new practical technology adopted in recent years. Cutting technology of test-tube seedlings has achieved good results in the ...

Claims

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Application Information

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IPC IPC(8): A01G17/00
Inventor 江文杨扬肖宝祥
Owner 天津樱桃谷农业科技发展有限公司
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