Tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings

A sweet cherry, fast technology, applied in the field of sweet cherry hybrid breeding, can solve the problems of deformed fruit rate limitation, abnormal germination and development, long emergence time, etc., to reduce damage, speed up the selection process, and increase the emergence rate.

Active Publication Date: 2020-08-04
HANGZHOU ACAD OF AGRI SCI
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Problems solved by technology

However, the tree species of "northern fruit moving southward" often have problems such as poor adaptability in production. The annual average temperature in the southern region is too high, especially high temperature and rainy summer, and insufficient low temperature in winter. The problem is more prominent. The problems of flowers but not fruit, abnormal germination and development, and high rate of deformed fruit have always restricted the development of the sweet cherry industry in the southern region.
The emergence rate of different methods is different, but the emergence time is longer

Method used

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  • Tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings
  • Tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings
  • Tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings

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Embodiment 1

[0076] Embodiment 1, a kind of method for tissue culture of sweet cherry intraspecific hybridization F1 generation fast seedling growth, comprises the following steps:

[0077] 1. Hybrid seed production and seed collection:

[0078] With "Yanyang" as the male parent, the large flower buds of the male parent that are about to open (ie Bell flower) were collected during the flowering period and brought back to the laboratory to strip off the anthers in time, spread them on sulfuric acid paper and dry them in the shade, and put them in glass vials for later use. With "Samituo" as the female parent, artificial emasculation and removal of petals were carried out during the flowering period of the bell, and the pollen of the male parent was used for point pollination, and no bagging was performed after pollination. After the fruit is picked at the mature stage, the core is taken, washed with water, and dried in the shade. After the seeds are collected, they are stored in a refrigera...

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Abstract

The invention discloses a tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings. The tissue culture method comprises hybridization seed production and seed collection. A collected seed comprises the following steps of in sequence: S1: carrying out seed hull-breaking processing and seed embryo dipping; S2: carrying out seed embryo disinfection and inner seed coat removal; S3: carrying out inoculation and culture; S4: checking an embryo pollution situation and secondary disinfection; S5: carrying out browning processing and timely transferring; andS6: carrying out acclimatization and transplantation. The tissue culture method only uses one MS solid culture medium which contains an exogenous growth promoting additive, time for taking roots fromgermination is short, the tissue culture method is simple and convenient to operate, hybridization F1-generation seedling growth time is greatly shortened, a whole seed breeding process is shortened,and Pruns avium L breeding efficiency is improved.

Description

technical field [0001] The invention relates to the field of hybrid breeding of sweet cherry, in particular to a method for promoting seedling formation of the F1 generation of intraspecific hybridization of sweet cherry by adopting a tissue culture method. Background technique [0002] Sweet cherry (Pruns avium L), commonly known as big cherry, is a perennial deciduous woody fruit tree belonging to Rosaceae (Rosaceae), Prunus L., and Cerasus Juss. It is the earliest mature deciduous fruit tree in northern my country. It is intensively cultivated In Tai'an, Yantai, Dalian and other places. Its fruit has the advantages of bright color, excellent flavor and quality, rich nutrition, green and pollution-free, high economic benefits, and high cultivation value. In recent years, it has gradually developed from the suitable planting area in the north to the south. The cultivation of sweet cherries in the southern region is mainly based on the selection of varieties suitable for pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张琛沈国正刘辉郗笃隽黄康康裴嘉博
Owner HANGZHOU ACAD OF AGRI SCI
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