Tissue Culture Method for Rapid Seedling Growth of Intraspecific Hybrid F1 Generation of Sweet Cherry

Abstract

The invention discloses a tissue culture method for rapid growth of sweet cherry intraspecific hybrid F1 generation seedlings, which includes hybrid seed production and seed collection; the collected seeds are sequentially subjected to the following steps: S1, seed breaking treatment and seed embryo soaking; S2 1. Embryo disinfection and inner testa removal; S3, inoculation and cultivation; S4, inspection of embryo contamination and secondary disinfection; S5, browning treatment and timely transfer; S6, seedling hardening and transplanting. The present invention only uses a MS solid medium containing exogenous growth-promoting additives, the time required from germination to rooting is relatively short, the operation is simple and convenient, and the time for seedling formation of the hybrid F1 generation is greatly shortened, the entire breeding process is shortened, and sweetness is improved. Cherry breeding efficiency.

Description

technical field

[0001] The invention relates to the field of hybrid breeding of sweet cherry, in particular to a method for promoting seedling formation of the F1 generation of intraspecific hybridization of sweet cherry by adopting a tissue culture method. Background technique

[0002] Sweet cherry (Pruns avium L), commonly known as big cherry, is a perennial deciduous woody fruit tree belonging to Rosaceae (Rosaceae), Prunus L., and Cerasus Juss. It is the earliest mature deciduous fruit tree in northern my country. It is intensively cultivated In Tai'an, Yantai, Dalian and other places. Its fruit has the advantages of bright color, excellent flavor and quality, rich nutrition, green and pollution-free, high economic benefits, and high cultivation value. In recent years, it has gradually developed from the suitable planting area in the north to the south. The cultivation of sweet cherries in the southern region is mainly based on the selection of varieties suitable for pla...

Examples

Embodiment 1

[0076] Embodiment 1, a kind of method for tissue culture of sweet cherry intraspecific hybridization F1 generation fast seedling growth, comprises the following steps:

[0077] 1. Hybrid seed production and seed collection:

[0078] With "Yanyang" as the male parent, the large flower buds of the male parent that are about to open (ie Bell flower) were collected during the flowering period and brought back to the laboratory to strip off the anthers in time, spread them on sulfuric acid paper and dry them in the shade, and put them in glass vials for later use. With "Samituo" as the female parent, artificial emasculation and removal of petals were carried out during the flowering period of the bell, and the pollen of the male parent was used for point pollination, and no bagging was performed after pollination. After the fruit is picked at the mature stage, the core is taken, washed with water, and dried in the shade. After the seeds are collected, they are stored in a refrigera...

technical field

[0001] The invention relates to the field of hybrid breeding of sweet cherry, in particular to a method for promoting seedling formation of the F1 generation of intraspecific hybridization of sweet cherry by adopting a tissue culture method. Background technique

[0002] Sweet cherry (Pruns avium L), commonly known as big cherry, is a perennial deciduous woody fruit tree belonging to Rosaceae (Rosaceae), Prunus L., and Cerasus Juss. It is the earliest mature deciduous fruit tree in northern my country. It is intensively cultivated In Tai'an, Yantai, Dalian and other places. Its fruit has the advantages of bright color, excellent flavor and quality, rich nutrition, green and pollution-free, high economic benefits, and high cultivation value. In recent years, it has gradually developed from the suitable planting area in the north to the south. The cultivation of sweet cherries in the southern region is mainly based on the selection of varieties suitable for pla...

Examples

Embodiment 1

[0076] Embodiment 1, a kind of method for tissue culture of sweet cherry intraspecific hybridization F1 generation fast seedling growth, comprises the following steps:

[0077] 1. Hybrid seed production and seed collection:

[0078] With "Yanyang" as the male parent, the large flower buds of the male parent that are about to open (ie Bell flower) were collected during the flowering period and brought back to the laboratory to strip off the anthers in time, spread them on sulfuric acid paper and dry them in the shade, and put them in glass vials for later use. With "Samituo" as the female parent, artificial emasculation and removal of petals were carried out during the flowering period of the bell, and the pollen of the male parent was used for point pollination, and no bagging was performed after pollination. After the fruit is picked at the mature stage, the core is taken, washed with water, and dried in the shade. After the seeds are collected, they are stored in a refrigera...

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