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SSR marker for identifying germplasm of prunus pauciflora, application and identifying method

A Chinese cherry and identification method technology, applied in the field of plant molecular marker-assisted germplasm resource identification and breeding, can solve the problem of inability to accurately identify Chinese cherry germplasm and other problems, and achieve the effects of low cost, good reproducibility and high accuracy

Active Publication Date: 2020-11-20
SICHUAN AGRI UNIV
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: to overcome the shortcomings of the inability to accurately identify Chinese cherry germplasm in the prior art, and to provide a specific SSR marker and method for identifying Chinese cherry germplasm

Method used

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  • SSR marker for identifying germplasm of prunus pauciflora, application and identifying method
  • SSR marker for identifying germplasm of prunus pauciflora, application and identifying method
  • SSR marker for identifying germplasm of prunus pauciflora, application and identifying method

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Embodiment Construction

[0031] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples and accompanying drawings. As a limitation of the present invention.

[0032] The present invention provides a kind of SSR marker primer of Chinese cherry germplasm, including forward primer and reverse primer, the nucleotide sequence of the forward primer is shown in SEQ ID NO.1, and the nucleotide sequence of the reverse primer is The acid sequence is shown in SEQ ID NO.2.

[0033] The nucleotide sequence of said SEQ ID NO.1 is: CGAACCTAAGACTACTGATCTA.

[0034] The nucleotide sequence of said SEQ ID NO.2 is: CTTGCTGCTTGTGGTAGAG.

[0035] Molecular markers and methods for identifying Chinese cherry germplasms, using the following Chinese cherry germplasms and related species as examples.

[0036] 1. Materials and methods

[0037] 1.1. Test materials:

[0038] In this embo...

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Abstract

The invention discloses an SSR marker for identifying germplasm of prunus pauciflora, application and an identifying method, and belongs to the technical field of plant molecular marker assisted germplasm resource identification and breeding. The SSR marker 78A9-1 is used as a molecular marker, genome DNA of germplasm of prunus pauciflora and affinis specifies is extracted, a pair of SSR marker primers is used for PCR amplification, an amplified product is subjected to polyacrylamide gel electrophoresis, spectrum bands are read, and the statistical analysis is performed. The invention overcomes the defect that the germplasm of the prunus pauciflora cannot be identified accurately, and provides the SSR marker for rapidly and accurately identifying the germplasm of the prunus pauciflora. TheSSR marker has the advantage of quickly and accurately identifying the germplasm of the prunus pauciflora.

Description

technical field [0001] The invention relates to the technical field of identification and breeding of germplasm resources assisted by plant molecular markers, in particular to an SSR marker, application and identification method for identifying germplasm of Chinese cherry. Background technique [0002] Chinese cherry is a perennial woody fruit tree of the genus Cerasus in the family Rosaceae. It has been cultivated for 3,000 years. Its fruit has a strong flavor and bright appearance, and has extremely high nutritional, health and economic values. However, Chinese cherry is a polyploid species with high genome heterozygosity and long childhood. There are homonyms and different names with other closely related species of the genus Cherry, and interspecies hybridization is prone to occur among species of the genus Cherry. The qualitative genetic composition is difficult to ascertain. [0003] Plant species identification is divided into field trait identification and molecular...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 王小蓉刘针杉王燕张静陈清杨绍凤汤浩茹陈涛王浩
Owner SICHUAN AGRI UNIV
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