Molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana

A M. incognita, molecular marker technology, applied in biochemical equipment and methods, microbial assay/test, DNA/RNA fragment, etc. Problems such as the breeding process of new varieties of cherry plum resistant to southern root-knot nematode

Pending Publication Date: 2021-12-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to breed a large number of wild cherry plum plants, and it takes a long time, low efficiency, poor stability, etc., which greatly affects the breeding process of

Method used

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  • Molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana
  • Molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana
  • Molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Molecular Markers for Predicting Resistance to Meloidogyne incognita Xinjiang Wild Cherry Plum

[0046] 1. Breeding of wild cherry plums and plants resistant to root-knot nematode incognita. Taking the wild cherry plum in Tianshan Mountains of Xinjiang as the original resource, the seeds were collected, and the seedling population was obtained by sowing. Multi-year identification of resistance of seedling populations to Meloidogyne incognita. The individual plants 'K-323', 'K-325', 'K-510' resistant to M. incognita and 'G-319', 'G-333', 'G-348' susceptible to M. incognita were obtained '. Grafting compatibility tests on single plants with high resistance to root-knot nematode in Xinjiang showed that wild cherry plums with high resistance to root-knot nematode in Xinjiang had good grafting compatibility with stone fruit trees such as peaches, plums, and apricots. It shows that the resistant rootstock provided by the application can be widely u...

Embodiment 2

[0076] Example 2 Method for Predicting Meloidogyne incognita in Xinjiang Wild Cherry Plum Using Molecular Markers

[0077] This example provides the application method of molecular markers of Xinjiang wild cherry plums resistant to root-knot nematode incognita. The Xinjiang wild cherry plums used in this example are 'K-325', 'K-510' and individual plants 'G-319', 'G-333', 'G-348', 'G-402 susceptible to Meloidogyne incognita '.

[0078] 1. Using the same method as in step 2 of Example 1, carry out the extraction of Xinjiang wild cherry plum genome cDNA.

[0079] 2. Using the oligonucleotide primers SEQ ID No.1-2 designed in Example 1 as PCR amplification primers.

[0080] 3. PCR polymerase chain reaction amplification and agarose gel electrophoresis detection:

[0081] (1) PCR reaction system: 2×M5HiPer plus Taq HiFi PCR Mix 12.5μl; cDNA template 2.5μl; SEQ ID No.1 1.25μl; SEQ ID No.2 1.25μl; deionized water 7.5μl;

[0082] (2) PCR reaction conditions: pre-denaturation at 9...

Embodiment 3

[0085] Example 3 Molecular Marker Prediction Accuracy of Xinjiang Cherry Plum's Resistance to Meloidogyne incognita and Grafting Affinity

[0086] EXAMPLES The primers (SEQ ID No.1-2) designed in Example 1 were used to predict the resistance to root-knot worm and grafting affinity of 30 Xinjiang cherry plums, and the results are shown in Table 1.

[0087] Table 1 The results of prediction using SEQ ID No.1-2

[0088]

[0089]

[0090]

[0091] As can be seen from the results in Table 1, using the primer pair shown in SEQ ID No.1-2, carry out the resistance prediction of root-knotworm for 30 strains of Xinjiang wild cherry plum, and the prediction accuracy rate reaches 100%; Confirm that the molecule provided by the present invention The markers are significantly correlated with the resistance to root-knot worm incognita. The primer pair provided by the invention can not only be used to predict the resistance to root-knot worm in Xinjiang wild cherry plums, but also can ...

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Abstract

The invention relates to the technical field of molecular markers, and particularly relates to a molecular marker for predicting Meloidogyne incognita resistance of Prunus sogdiana. The invention provides a primer for detecting the molecular marker, and the primer has a nucleotide sequence shown as SEQ ID No. 1-2. A method for predicting Meloidogyne incognita resistance of Prunus sogdiana by using the primer provided by the invention comprises the following steps of: extracting RNA of leaves of Prunus sogdiana and reversing the RNA into cDNA; performing PCR amplification by using the primer shown as SEQ ID No. 1-2; and detecting a PCR product by agarose gel electrophoresis. According to the invention, resistance evaluation is carried out without inoculating Meloidogyne incognita, resistant plants can be screened out, and important scientific guiding significance is provided for identification and variety pre-selection of excellent rootstocks resistant to Meloidogyne incognita.

Description

technical field [0001] The invention relates to the field of plant molecular breeding, in particular to a molecular marker for predicting the resistance of Xinjiang wild cherry plums to root-knot nematode incognita. Background technique [0002] Xinjiang wild cherry plum (Prunus sogdiana) is a wild fruit tree, which has the characteristics of tolerance to poor soil, salt and alkali, humidity and cold. Xinjiang wild cherry plums are propagated asexually, and the survival rate of cuttings of hard branches and green branches is above 80%. In addition, Xinjiang wild cherry plums have good grafting compatibility with stone fruit trees such as peaches, plums, and apricots, and are the preferred material for rootstocks of stone fruit trees. [0003] DNA molecular marker is a marker method that can detect and analyze a specific DNA fragment in the plant genome. It has many advantages such as not being affected by environmental conditions, not being affected by plant growth stages,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 胡建芳蒲文江肖坤高朝远罗嗣芳
Owner CHINA AGRI UNIV
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