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Gene chip used for identifying pathogenic bacteria in blood and its making method

A technology of gene chips and pathogenic bacteria, applied in the field of gene chips, can solve the problems of missing pathogenic bacteria and poor accuracy, and achieve the effects of accurate diagnosis, avoiding the cultivation stage, and high information content

Inactive Publication Date: 2003-04-30
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fewer probes are included, only 22 types of pathogenic bacteria are covered, many pathogenic bacteria will be missed, and the accuracy is poor

Method used

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  • Gene chip used for identifying pathogenic bacteria in blood and its making method
  • Gene chip used for identifying pathogenic bacteria in blood and its making method
  • Gene chip used for identifying pathogenic bacteria in blood and its making method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] 1. Design of Pathogen-Specific Probes

[0031] The 23S ribosomal RNA gene of bacteria was selected as the target sequence, and species, genus-specific and general sequences were selected from it to design probes for the detection of pathogenic bacteria. The probes are shown in the sequence listing.

[0032] 2. Synthetic probes: Synthesized using a synthesizer from Shanghai Bioengineering Co., Ltd.

[0033] 3. Probe treatment: Since the designed probe is relatively short, it is difficult to solidify on the membrane, so we selectively added a polyT (oligo-deoxythymonucleotide) tail to the 3' end, so that it can be solidified on the nylon membrane superior.

[0034] 4. Preparation of gene chip

[0035] Use the nylon membrane with positive point load produced by Roche, and use the sample spotter to spot samples in a preset order.

[0036] 5. Cross-link the spotted membrane under a long-wave ultraviolet lamp for 5 minutes, so that the probe is firmly fixed on the membran...

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PUM

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Abstract

A gene chip for detecting the pathogenic bacteria from blood features that the specific probes, derivative probes, complementary probes, and universal probes are arranged on the nylon film to detect different pathogenic bacteria. Its advantages are high speed and high correctness.

Description

Technical field [0001] The invention relates to medical in vitro diagnostic technology, specifically a gene chip, on which probes for various bacteria, including some bacteria that are difficult to separate by conventional culture, are used for hybridization test detection and identification test using PCR amplification products sample. Background technique [0002] Isolation of bacteria from blood usually indicates a serious infection in a patient requiring urgent antimicrobial therapy. Different bacteria require different antibiotics to treat, and successful treatment depends on the timely use of the correct drugs. The current microbial identification methods for clinical application are based on physiological and biochemical bioassays, which require the cultivation and isolation of microorganisms in clinical samples. Generally, the culture medium will show positive after 8-12 hours after the patient's blood is drawn and cultured. At this time, Gram staining can be used ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 黄熙泰黄志刚
Owner NANKAI UNIV
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