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Method for establishing coltsfoot flower medicine fingerprint and standard fingerprint thereof

A standard fingerprint and fingerprint technology, which is applied in the field of pharmaceutical analysis, can solve problems such as inability to systematically and completely reflect the internal quality of coltsfoot flowers, and achieve the effects of easy realization of chromatographic conditions, simple production, good stability and reproducibility

Inactive Publication Date: 2012-06-27
TAISHAN MEDICAL UNIV
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Problems solved by technology

The quality control of coltsfoot in Chinese Pharmacopoeia is limited to the content determination of coltsfoot, which cannot systematically and completely reflect the intrinsic quality of coltsfoot

Method used

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  • Method for establishing coltsfoot flower medicine fingerprint and standard fingerprint thereof
  • Method for establishing coltsfoot flower medicine fingerprint and standard fingerprint thereof

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Embodiment Construction

[0018] Preferred embodiments of the present invention are as follows:

[0019] (1) Instruments and drugs: Waters high-performance liquid chromatography was used; 10 batches of coltsfoot medicinal materials were purchased from pharmacies or medicinal materials markets; other reagents were chromatographically pure or analytically pure.

[0020] (2) Preparation of the test solution: Take 1.0 g of winter flower medicinal material powder, weigh it accurately, put it in a stoppered container, add 50 mL of ethanol accurately, ultrasonicate for 1 hour, and cool the supernatant through a 0.45 μm microporous filter Membrane filtration, the filtrate was used as the test solution.

[0021] (3) HPLC analysis: chromatographic separation column packing 5μm Diamonsil-C 18 , 250×4.6mm; mobile phase 0.1% phosphoric acid-acetonitrile; gradient elution method: 0min→30min→40min→50min→60min, acetonitrile 10%→25%→75%→100%→100%; flow rate 0.5~1.5 mL / min; detection wavelength 240nm; column tempe...

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Abstract

The invention provides a method for establishing a coltsfoot flower medicine HPLC (High Performance Liquid Chromatography) fingerprint and a standard fingerprint thereof. The method comprises the following steps of: taking coltsfoot flower medicine powder, adding ethanol, carrying out ultrasonic extraction, filtering supernate by using a micro-pore filter film, and taking the filtrate as a sample solution; then, carrying out high-performance liquid chromatography, wherein a chromatographic column is a Diamonsil-C18 column (5 mum, 250*4.6 mm); taking 0.1% of phosphoric acid-acetonitrile as a mobile phase, and adopting a gradient elution way; detecting the wavelength of 240 nm and the column temperature of 30 DEG C; introducing the sample by absorbing the sample solution to obtain the coltsfoot flower medicine HPLC fingerprint; and comparing 10 batches of coltsfoot flower medicine HPLC fingerprints and determining common fingerprint characteristics to obtain the standard fingerprint. The quality of coltsfoot flower medicine can be effectively monitored by utilizing the standard fingerprint, so that stability, uniformity and controllability of the coltsfoot flower medicine can be kept.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing fingerprints of coltsfoot medicinal materials high-performance liquid chromatography (HPLC), and a standard fingerprint of coltsfoot medicinal materials obtained by the method, belonging to the technical field of drug analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. At present, fingerprints have become one of the most effective means to identify the varieties of traditional Chinese medicines or evaluate the quality of traditional Chinese medicines recognized at home and abroad. In the "FDA Guidelines on Plant Products" formulated by the US Food an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 王福刚曹娟
Owner TAISHAN MEDICAL UNIV
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