Method for establishing HPLC fingerprint of dichloromethane part of root of costus speciosus (koening) smith as Yao medicinal material

A kind of technology of Dichloromethane and Fingerprint of Yimujiang

Active Publication Date: 2019-09-20
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0005] At present, there is no relevant report on the fingerp

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  • Method for establishing HPLC fingerprint of dichloromethane part of root of costus speciosus (koening) smith as Yao medicinal material
  • Method for establishing HPLC fingerprint of dichloromethane part of root of costus speciosus (koening) smith as Yao medicinal material
  • Method for establishing HPLC fingerprint of dichloromethane part of root of costus speciosus (koening) smith as Yao medicinal material

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Embodiment 1

[0061] The method for establishing the HPLC fingerprint of the dichloromethane of Yaomu ginger includes the following steps:

[0062] (1) Prepare the test solution: accurately weigh 2.0000 g of Mother ginger medicinal material, add 50ml of ethanol solution with a volume fraction of 70% accurately, weigh it, ultrasound for 60 min, let it cool and weigh it to make up for the weight loss After filtering, add 20 ml of 3mol / ml hydrochloric acid solution to the filtrate, reflux in a water bath for 2 hours, let it cool, adjust to neutral with 40% sodium hydroxide solution, evaporate to dryness in a water bath, and heat the residue with 30 ml Dissolve the water and transfer it to a separatory funnel for extraction with chloroform. Extract twice, 30 ml each time, combine the chloroform fractions and evaporate to dryness. The residue is made up to a 10 ml brown volumetric flask with methanol. Filter by 0.22 µm organic filter membrane, ready to be obtained;

[0063] (2) Prepare the reference...

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Abstract

The invention discloses a method for establishing an HPLC fingerprint of a dichloromethane part of the root of costus speciosus (koening) smith as a Yao medicinal material. The method comprises the following steps: (1), preparing a test solution; (2), preparing a reference solution by using diosgenin as a standard product; (3), carrying out HPLC detection; to be specific, recording an HPLC map within 95 minutes; and (4), detecting a plurality of test products, carrying out matching of all chromatographic peaks by using "Traditional Chinese medicine chromatographic fingerprint similarity evaluation system" software and a multi-point calibration method, and generating a reference fingerprint R automatically as a reference fingerprint. According to the invention, with establishment of the fingerprint of the root of costus speciosus (koening) smith, the relative relationship of the components contained in the root of costus speciosus (koening) smith can be reflected comprehensively; the medicinal materials from different origin places can be distinguished effectively; and the quality can be evaluated and controlled well. Therefore, the development and utilization of the root of costus speciosus (koening) smith in Guangxi province can be promoted.

Description

Technical field [0001] The invention relates to the technical field of fingerprint patterns of traditional Chinese medicinal materials, in particular to a method for establishing HPLC fingerprint patterns of dichloromethane parts of Yaomu ginger. Background technique [0002] Zingiber officinale Costus speciosus (Koen.) Smith The rhizome, a perennial herb, is one of the traditional Yao medicine. The Yao folks often use it as a nursing medicine for the parturients, which has a good recovery effect on the parturients. Therefore, the Yao people call it "Yinmu ginger" and "Guanyin ginger". Born in damp places such as valleys, under forests, roadsides along forest edges, and shrubs and grasses beside streams below 1700 m altitude. It is mainly distributed in Guangxi, Guangdong, Yunnan, Fujian, Taiwan and other provinces and regions, as well as in Southeast Asia and South Asian subtropical regions. Guangxi Yimu ginger is mainly distributed in Laibin, Baise, Guilin, Pingxiang, Guiping...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/86
CPCG01N30/8686G01N30/88G01N2030/8809
Inventor 王志萍黄杰连黎芳苏佳昇李晓霞蒋雅娴刘笑
Owner GUANGXI UNIV OF CHINESE MEDICINE
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