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Medlar HPLC fingerprint establishment method and its standard fingerprint

A standard fingerprint, fingerprint technology, applied in the field of drug analysis, can solve the problem of lack of quality of wolfberry, and achieve the effect of good stability and reproducibility

Inactive Publication Date: 2008-07-09
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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Problems solved by technology

At present, people's quality control of wolfberry is limited to qualitative and quantitative analysis of an active component or an index component among its many components, and there is a lack of standard control methods that fully reflect the quality of wolfberry.

Method used

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  • Medlar HPLC fingerprint establishment method and its standard fingerprint

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Embodiment Construction

[0016] Preferred embodiments of the present invention are as follows:

[0017] (1) Instruments and medicines: Shimadzu LC10A high-performance liquid chromatography was used; 21 batches of wolfberry medicinal materials were purchased from Chinese herbal medicine stores; other reagents were chromatographically pure or analytically pure.

[0018] (2) Preparation of the test solution: Accurately weigh 10 g of medicinal material powder, place it in a volumetric flask, add 500 mL of 80% methanol, oscillate ultrasonically for 60 min, and cool to constant volume. The supernatant was filtered with a 0.45 μm microporous membrane, and the filtrate was used as the test solution.

[0019] (3) HPLC analysis: chromatographic separation column packing is 5μm Kromasil-C18, 250×4.6mm; mobile phase is water-acetonitrile; gradient elution method: 0min→15min→30min→45min→60min, acetonitrile 10% →22%→35%→45%→55%; flow rate 1.0mL / min; detection wavelength 254nm; column temperature 30°C. Under these...

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Abstract

The invention provides a method for establishing an HPLC fingerprint spectrum of Fructus Lycii and a standard fingerprint spectrum thereof. The method comprises the following steps of: preparing a test solution, preparing a reference solution, selecting chromatographic conditions, and carrying out HPLC determination to obtain the fingerprint spectrum of Fructus Lycii. The standard fingerprint spectrum of Fructus Lycii can be obtained by comparing HPLC fingerprint spectra of 21 batches of Fructus Lycii materials and determining the common fingerprint characteristics. The method has the advantages of simple operation, good reproducibility, a plurality of characteristic peaks, accurate and reliable result, etc. The standard fingerprint spectrum can be used for effectively monitoring the quality of Fructus Lycii.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing fingerprints of high performance liquid chromatography (HPLC) of wolfberry medicinal materials, and a standard HPLC fingerprint of wolfberry obtained by the method. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. At present, fingerprints have become one of the most effective means to identify the varieties of traditional Chinese medicines and evaluate the quality of traditional Chinese medicines recognized at home and abroad. In the "FDA Guidelines on Plant Products" formulated by th...

Claims

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Application Information

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IPC IPC(8): G01N30/86G01N30/06G01N30/02G01N33/15
Inventor 周考文王树聪李凤桐
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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