Construction method of UPLC fingerprint of medicinal raw ginger and standard fingerprint thereof

A technology for ginger and medicinal materials, which is applied in the field of construction of ultra-high performance liquid chromatography (UPLC) fingerprints of ginger medicinal materials, can solve the problems of not being able to fully explain its quality status, etc., and achieves short sampling time, simple preparation method, and easy chromatographic conditions. Effect

Inactive Publication Date: 2014-11-26
韩燕全
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Problems solved by technology

At present, the quality control and quality evaluation methods of ginger mainly focus on the content determination of single or several components, which cannot fully explain its quality status, and technical methods that can comprehensively evaluate the quality of ginger are needed.

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  • Construction method of UPLC fingerprint of medicinal raw ginger and standard fingerprint thereof

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Embodiment Construction

[0016] (1) Instruments and medicines: Waters ultra-high liquid chromatography was used; 17 batches of ginger medicinal materials were collected from different production areas across the country, and the reagents used were all chromatographically pure or analytically pure.

[0017] (2) Preparation of the test solution: Accurately weigh about 1.25g of ginger (according to the water content determination method under the item of "2010 Edition (Appendix VIII), determine the water content with the toluene method), put it in a 10ml volumetric flask, add methanol to the mark, Ultrasound (150W, 40Hz) for 25min, let cool, weigh, add methanol to make up for weight loss, shake well, filter through a 0.22μm filter membrane, and take the subsequent filtrate to obtain the final product.

[0018] (3) Ultra-high liquid chromatography analysis: The chromatographic separation column is a UPLC column, and the packing is a BEHC18 column (2.1 mm×100 mm, 1.7 μm); acetonitrile (A)-water solution (B)...

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Abstract

The invention provides a construction method of ultrahigh performance liquid chromatography (UPLC) fingerprint of medicinal raw ginger, and belongs to the technical field of pharmaceutical analysis. The method is characterized by comprising the following steps: (1) precisely weighing about 1.25 grams of raw ginger, placing the raw ginger into a volumetric flask with a volume of 10 mL, adding methanol until the methanol reaches the scale, subjecting the volumetric flask to a supersonic treatment for 25 minutes, cooling, weighing, adding methanol to compensate the weight loss, shaking, filtering with a filter membrane with a pore size of 0.22 [mu]m, saving the filtrate for later use; (2) fingerprint preparation: adopting an ultrahigh performance liquid chromatography (UPLC) system, wherein the chromatographic column is a UPLCBEHC18 chromatographic column (2.1mm*100mm, 1.7[mu]m); acetonitrile (A)-water solution (B) is the mobile phase, gradient elution is adopted, the detection wavelength is 280 nm, the flow speed is 0.35 mL.min<-1>, and the column temperature is 35 DEG C, carrying out analysis so as to obtain UPLC fingerprint of medicinal raw ginger; (3) determination of standard fingerprint: establishing UPLC fingerprints of 17 batches of medicinal raw gingers from different production areas, and determining 16 common characteristic peaks, wherein the fingerprint characteristic of medicinal raw ginger is composed of the 16 common characteristic peaks, and can be used as the standard fingerprint of medicinal raw ginger.

Description

technical field [0001] The invention designs a method for constructing fingerprints of traditional Chinese medicinal materials, in particular a method for constructing ultra-high performance liquid chromatography (UPLC) fingerprints of ginger medicinal materials and standard fingerprints thereof, belonging to the technical field of drug analysis. Background technique [0002] Traditional Chinese medicine is a multi-component complex system with rich chemical components. At the same time, its quality will be affected by many aspects such as the origin, variety, transportation, processing and other aspects of traditional Chinese medicine. Therefore, the research on the quality control of traditional Chinese medicine and its preparations has always been a The bottleneck of the modernization of TCM and its preparations and its internationalization has always been a top priority in the research of traditional Chinese medicine and its preparations, and it is also a difficult point....

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 韩燕全夏伦祝汪永忠
Owner 韩燕全
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