Establishment method for high performance liquid chromatography fingerprint of Rubia cordifolia root formula granule and standard fingerprint thereof

Abstract

The invention provides an establishment method for a high performance liquid chromatography (HPLC) fingerprint of a Rubia cordifolia root formula granule, and a Rubia cordifolia root formula granule standard fingerprint obtained thereby. The establishment method provided by the invention includes the steps of: (1) preparation of a test solution; and (2) high performance liquid chromatography analysis. The high performance liquid chromatography standard fingerprint of the Rubia cordifolia root formula granule aqueous solution obtained by the method has a total of 11 chromatographic peaks, the retention time of which is 6.072min, 6.559min, 8.087min, 9.170min, 12.118min, 20.988min, 29.251min, 32.211min, 33.189min, 38.430min and 45.431min respectively. The HPLC standard fingerprint of the Rubia cordifolia root formula granule fat-soluble components has a total of 9 chromatographic peaks, the retention times of which is 12.156min, 12.832min, 14.552min, 22.615min, 23.291min, 25.412min, 27.356min, 34.383min and 37.076min respectively. According to the invention, the test solution is simple to prepare, the chromatographic conditions are easy to realize, and the method has good stability and reproducibility, can effectively control the quality of the Rubia cordifolia root formula granule, and provides reference for quality control of the Rubia cordifolia root formula granule.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of traditional Chinese medicine formula granules, in particular to a method for establishing high performance liquid chromatography fingerprints of madder formula granules, and the standard fingerprint of madder formula granules obtained by the method, belonging to the technical field of drug analysis. Background technique [0002] Rubia formula granules are obtained by extracting, concentrating and granulating the traditional Chinese medicine Rubia. Rubia is the dry root and rhizome of Rubia cordifolia L., which is mainly produced in Anhui, Jiangsu, Shandong, Henan and Shaanxi. Excavated in spring and autumn, removed the sediment and dried. Radix Rubiae is bitter and cold in nature and flavor, returns to Liver Meridian, is used for cooling blood, dispelling blood stasis, hemostasis, stimulates menstrual flow, and cures mainly hematemesis, epistaxis, metrorrhagia, traumatic hemorrhage, am...

AI Technical Summary

Problems solved by technology

[0003] The quality control of madder in the 2010 edition of "Chinese Pharmacopoeia" includes items such as original plant species, medicinal properties, physical and chemical identification, and content determination. naphthaquinones such as rubidin, rubidolide and other naphthoquinones and their glycosides, cyclohexyl peptides, triterpenoids, polysaccharides and other ingredients, but for traditional Chinese medicine preparations such as madder formula granules with many types of active ingredients and complex components, only It is difficult to objectively and effectively evaluate or control the quality of medicinal materials by determining the content of certain types of ingredients

However, there is no report on the testing and analysis of the fingerprints of madder formula granules by using high performance liquid chromatography (HPLC).

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