Construction method of UPLC fingerprint of sweet clover medicinal material and standard fingerprint thereof

A standard fingerprint and construction method technology, applied in the field of drug analysis, can solve the problems of not being able to effectively reflect and control the overall quality of Medicinal Rhinoceros, and achieve the effects of shortening online analysis time, high precision, and more peaks

Active Publication Date: 2016-06-22
STAIDSON (BEIJING) BIOPHARMACEUTICALS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The construction method according to the present invention solves the problem that the overall quality of Medicinal Rhizome cannot be effectively reflected and controlled in the component analysis of Medicinal Rhizome, and makes up for the deficiency of existing quality control technology

Method used

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  • Construction method of UPLC fingerprint of sweet clover medicinal material and standard fingerprint thereof
  • Construction method of UPLC fingerprint of sweet clover medicinal material and standard fingerprint thereof
  • Construction method of UPLC fingerprint of sweet clover medicinal material and standard fingerprint thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Instruments and medicines

[0041] Use WatersACQUITYUPLCH-Class ultra-high performance liquid chromatography. A total of 10 batches of sweet rhinoceros medicinal materials were purchased from Anhui Mengshi Pharmaceutical Co., Ltd., and the coumarin reference substance (EP standard) was purchased from ChromaDax Co., Ltd. (ChromaDax, Inc.). Other reagents were chromatographically pure or analytically pure.

[0042] 2. Preparation of test sample solution

[0043] Precisely weigh 2.0000g of medicinal material powder, put it in a stoppered container, add 90mL of 70% by volume methanol aqueous solution to soak, oscillate ultrasonically for 30 minutes, let cool, filter, collect medicinal residues, repeat the extraction once and combine twice according to the above method The extracted filtrate was put into a 250ml measuring flask, adjusted to the mark with methanol-water (volume ratio 1:1), and filtered with a 0.22 μm filter membrane, and the filtrate was used as the test ...

Embodiment 2

[0058] 1. Instruments and medicines used

[0059] Use WatersACQUITYUPLCH-Class ultra-high performance liquid chromatography. A total of 10 batches of Medicinal Rhizoma Medicinae were purchased from Anhui Mengshi Pharmaceutical Co., Ltd., the coumarin reference substance (EP standard) was purchased from ChromaDax Co., Ltd. (ChromaDax, Inc.), and other reagents were of chromatographic or analytical grade reagent.

[0060] 2. Preparation of test sample solution

[0061] Accurately weigh 2.0000g of sweet rhinoceros medicinal powder, put it in a stoppered container, add 90mL of 50% methanol aqueous solution to soak, oscillate ultrasonically for 30 minutes, let cool, filter, then collect the medicinal residues, repeat the extraction once again according to the above method, and combine Put the filtrate extracted twice into a 250mL volumetric flask, dilute to the mark with methanol-water (volume ratio 1:1), filter with a 0.22μm filter membrane, and use the filtrate as the test samp...

Embodiment 3

[0088] 1. Instruments and medicines

[0089] Use WatersACQUITYUPLCH-Class ultra-high performance liquid chromatography. A total of 10 batches of sweet rhinoceros medicinal materials were purchased from Anhui Mengshi Pharmaceutical Co., Ltd., and the coumarin reference substance (EP standard) was purchased from ChromaDax Co., Ltd. (ChromaDax, Inc.). Other reagents were chromatographically pure or analytically pure.

[0090] 2. Preparation of test sample solution

[0091] Accurately weigh 2.0000g of medicinal material powder, put it in a stoppered container, add 90mL of 30% by volume methanol aqueous solution to soak, oscillate ultrasonically for 30 minutes, let cool, filter, collect medicinal residues, repeat the extraction once and combine twice according to the above method The extracted filtrate was put into a 250ml measuring flask, adjusted to the mark with methanol-water (volume ratio 1:1), and filtered with a 0.22 μm filter membrane, and the filtrate was used as the test...

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Abstract

The invention provides a method for constructing the UPLC fingerprint of Medicinal Rhizome and the standard fingerprint of Medicinal Rhododendron obtained by the method. The method for constructing the UPLC fingerprint of Medicinal Rhizome according to the present invention includes the steps of preparing the test sample solution and the step of ultra-high performance liquid chromatography analysis. According to the method of the present invention, the method is simple, the chromatographic analysis conditions are easy to realize, the precision is high, and the stability and reproducibility of the analysis results are better. Compared with other test and analysis methods in the prior art, the online analysis time is shortened and the Material cost and time cost.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing ultra-high performance liquid chromatography (UPLC) fingerprints of medicinal materials of Rhododendron meliflora and standard fingerprints thereof. Background technique [0002] Sweet clover is also written as sweet clover, which belongs to the genus Melilotus Miller of the Fabaceae family, an annual or biennial herb. Osmanthus fragrans is often born in sand dunes, hillsides, grasslands, and seasides, and is distributed in most provinces and regions of my country. It is also known as Pihan grass, Shengtou grass, wild alfalfa and iron broom. Currently cultivated in my country are mostly white-flowered Osmanthus and yellow-flowered Osmanthus. Osmanthus chrysanthemum is used as a good spice raw material in China because of its own growth advantages and suitable chemical composition. The whole herb can be used as medicine, and the leaves and flowers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
Inventor 张丹冯彩丽徐江平
Owner STAIDSON (BEIJING) BIOPHARMACEUTICALS CO LTD
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