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Method for establishing HPLC (high performance liquid chromatography) finger-print of ophiopogon japonicus and standard finger-print thereof

A standard fingerprint and fingerprint technology, applied in the field of drug analysis, can solve the problems of completely indicating the overall quality of Ophiopogon japonicus, single quality evaluation method, etc., and achieves easy realization of chromatographic conditions, simple production, stability and reproducibility. Good results

Inactive Publication Date: 2011-07-13
刘江 +1
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Problems solved by technology

Ophiopogon japonicus contains a variety of chemical components. Since it was reported in 1936 that the roots of Ophiopogon japonicus contained vitamin A compounds, compounds such as sugars, saponins, and flavonoids have been isolated from Ophiopogon japonicus successively; The single evaluation method is not enough to systematically and completely indicate the overall quality of Ophiopogon japonicus, and it is urgent to explore more advanced and effective quality control methods

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  • Method for establishing HPLC (high performance liquid chromatography) finger-print of ophiopogon japonicus and standard finger-print thereof
  • Method for establishing HPLC (high performance liquid chromatography) finger-print of ophiopogon japonicus and standard finger-print thereof

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Embodiment Construction

[0017] Preferred embodiments of the present invention are as follows:

[0018] (1) Instruments and medicines: Agilent 1101 high performance liquid chromatography was used; 16 batches of Ophiopogon japonicus medicinal materials were collected in Santai County, Mianyang City, Sichuan Province, the authentic production area of ​​Ophiopogon japonicus; other reagents were chromatographically pure or analytically pure.

[0019] (2) Precisely weigh 3g of Ophiopogon japonicus sample into a stoppered container, add 50ml of 95% ethanol aqueous solution, ultrasonically shake (20°C, 90min), filter through a filter membrane, and rotate the filtrate to 2-3ml (50°C , 80r / min) was dissolved in 40ml of water, transferred to a centrifuge tube, extracted with ether 4 times, 20ml each time, the ether layer was rotary evaporated to 3-5ml, methanol was fixed to 10ml, passed through a 0.45μm disposable filter membrane, and the filtrate was used as The test solution.

[0020] (3) High-performance li...

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Abstract

The invention provides a method for establishing an HPLC (high performance liquid chromatography) finger-print of ophiopogon japonicus and a standard finger-print thereof. The method comprises the following steps of: preparing a test article solution; preparing a reference substance solution; selecting chromatographic conditions; and measuring with a high performance liquid chromatograph to obtain the finger-print of ophiopogon japonicus. 16 batches of HPLC finger-prints of ophiopogon japonicus are compared to determine the common finger-print characteristics of ophiopogon japonicus, thereby obtaining the standard finger-print. The method provided by the invention is simple, convenient, accurate and reliable, and has the characteristics of good reproducibility, multiple characteristic peaks and the like. The established standard finger-print can effectively show the quality of ophiopogon japonicus.

Description

technical field [0001] The invention relates to a method for constructing fingerprints of Chinese medicinal materials, in particular to a method for constructing fingerprints of high-performance liquid chromatography (HPLC) of Ophiopogon japonicus and standard fingerprints thereof. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. As a comprehensive and quantifiable quality control method, traditional Chinese medicine fingerprinting has been recognized by many international medical institutions and has become one of the most effective methods for quality control of Chinese patent medicines and natural medicines. In the "FDA Guidelines on Plant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8968A61P11/14A61P1/10A61P25/20A61P43/00G01N30/02G01N30/86A61K125/00
Inventor 刘江陈兴福
Owner 刘江
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