Construction method of rheum lhasaense fingerprint spectrum and standard fingerprint spectrum of rheum lhasaense

A fingerprint, Lhasa rhubarb technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of insufficient quality evaluation research of Lhasa rhubarb, difficult to fully reflect the quality of medicinal materials, restricting the industrialization of Lhasa rhubarb, etc. The effect of quality monitoring, easy realization of chromatographic conditions, and simple method

Active Publication Date: 2017-01-04
KPC PHARM INC
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Problems solved by technology

[0005] So far, the research on the quality evaluation of Lhasa rhubarb is not enough, and the lack of standards has become a key problem and bottleneck problem restricting the industrialization of Lhasa rhubarb
An Lizhen et al established the content determination method of polydatin, methylpolydatin and tristilbene in Lhasa rhubarb by ultra-high performance liquid chromatography (An Lizhen et al. Determination of three chemical components in Lhasa rhubarb by ultra-high performance liquid chromatography content, Yunnan College of Traditional Chinese Medicine, 2013, 36(1): 31-34.), but its quality evaluation method is limited to the content determination of several index components, which is difficult to fully reflect the quality of medicinal materials

Method used

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  • Construction method of rheum lhasaense fingerprint spectrum and standard fingerprint spectrum of rheum lhasaense
  • Construction method of rheum lhasaense fingerprint spectrum and standard fingerprint spectrum of rheum lhasaense
  • Construction method of rheum lhasaense fingerprint spectrum and standard fingerprint spectrum of rheum lhasaense

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preparation example Construction

[0037] (1) Preparation of reference substance solution:

[0038] Preparation of Tristilbene Reference Substance Solution: Accurately weigh an appropriate amount of Tristilbene Reference Substance, use 50% to 100% methanol (volume percentage, the following methanol concentrations are volume percentages), preferably 75% methanol, and prepare the solution for every 1ml A solution containing 10-1000 μg of tristilbene, preferably 100 μg of tristilbene per 1 ml.

[0039] Preparation of Polydatin Reference Substance Solution: Accurately weigh an appropriate amount of Polydatin Reference Substance, and use 50% to 100% methanol, preferably 75% methanol, to prepare a solution containing 10 to 1000 μg of Polydatin per 1 ml, preferably Quzha per 1 ml Stilbene 100 μg.

[0040] Preparation of deoxyrhein reference substance solution: Accurately weigh an appropriate amount of deoxyrhein reference substance, and use 50% to 100% methanol, preferably 75% methanol, to prepare a solution containi...

Embodiment

[0065] 1 Instruments and materials

[0066] 1.1 Main Instruments

[0067] Agilent 1200 high performance liquid chromatography (including online degasser, quaternary pump, autosampler, DAD detector) (Agilent), CP225D analytical balance (Sartorius), BP221S analytical balance (Sartorius), Milli- Q ultrapure water machine (Millipore Company), 5510E-DTH ultrasonic cleaner (Branson Company, USA), and BuchiR-3 rotary evaporator (Buchi Company).

[0068] 1.2 Experimental materials

[0069] Reagents: chromatographically pure acetonitrile (Germany Merck & Co., Ltd.), chromatographically pure methanol (Germany, Merck & Co., Ltd.), chromatographically pure ethanol (Thermo Fisher Scientific Co., Ltd.), formic acid (analytical grade), phosphoric acid ( Analytical grade), ultrapure water, tristilbene (made by Kunming Pharmaceutical Research Institute, purity ≥ 98%), polydatin (made by Kunming Pharmaceutical Research Institute, purity ≥ 98%), Deoxyreveretin (made by the Pharmaceutical Rese...

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Abstract

The invention provides a construction method of a rheum lhasaense fingerprint spectrum and a standard fingerprint spectrum of rheum lhasaense. High performance liquid chromatograph is conducted on a rheum lhasaense sample through a chromatographic column taking octadecylsilane chemically bonded silica as packing, the fingerprint spectrum is constructed by taking piceatannol 3'-O-glucoside as a reference peak, and the fingerprint spectrum is evaluated on this basis. The obtained rheum lhasaense fingerprint spectrum can comprehensively, systematically and characteristically reflect composition ingredients in rheum lhasaense, comprehensively and effectively evaluate the quality of rheum lhasaense and objectively reflect authenticity and merits of a product and is beneficial for product quality monitoring. The method is easy and convenient to operate, stable, high in precision and good in reproducibility.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and in particular relates to a method for constructing a Lhasa rhubarb fingerprint and a standard fingerprint thereof. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum of a certain type or several types of components that are common in certain Chinese medicinal materials or Chinese patent medicines. At the present stage, when most of the active ingredients of traditional Chinese medicine are not clear, the fingerprint of traditional Chinese medicine is of great significance for the effective control and evaluation of the quality of Chinese medicinal materials or Chinese patent medicines. [0003] Rhubarb Lhasa is the dry root and rhizome of Rheum lhasaense A.J.Li et P.K.Hsiao, a plant of the Polygonaceae family. It is recorded in Flora of Tibet. It has the effects of purging heat and poison, breaking stagnatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李宁龚云麒方芳杨兆祥
Owner KPC PHARM INC
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