22 results about "Protocatechuic aldehyde" patented technology

The invention provides a content detection method for a salvia miltiorrhiza medicinal material, a salvia miltiorrhiza clear paste and a musk heart-dredging dropping pill. According to the method, a high performance liquid chromatography method is adopted, octadecylsilane chemically bonded silica is used as a chromatographic column filler, acetonitrile is used as a mobile phase A, 0.02%-0.1% trifluoroacetic acid is used as a mobile phase B, gradient elution is carried out, and rosmarinic acid and salvianolic acid B in the salvia miltiorrhiza medicinal material are synchronously and quantitatively detected. And synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid and salvianolic acid B in the salvia miltiorrhiza clear paste, and synchronously and quantitatively detecting tanshinol, protocatechuic aldehyde, caffeic acid, salvianolic acid E, rosmarinic acid, alkannic acid, salvianolic acid B, arenobufagin and bufalin in the musk Tongxin dropping pill. The method is used for monitoring the content of specific components, can also judge the transfer rate in the medicine production process, and facilitates traceability in the production link. In addition, the method is relatively low in equipment requirement and suitable for industrial popularization.

The invention relates to the technical field of medical nano-materials, and discloses an iron-manganese bimetallic MOFs nano-enzyme hydrogel compound and a preparation method and application thereof, and the method comprises the following steps: step 1, preparing FeMn bimetallic MOFs nano-enzyme MIL-101 by adopting a microwave-assisted solvothermal method; step 2, preparing oxidized hyaluronic acid; step 3, synthesizing a hydrogel precursor protocatechuic aldehyde and a complex PA-Fe of iron ions; and step 4, fully mixing the quaternary ammonium salt chitosan solution with the PA-Fe solution, adding the oxidized hyaluronic acid solution and the MIL-101 powder under continuous magnetic stirring, and finally forming the iron-manganese bimetal MOFs nano-enzyme hydrogel compound. The delivery efficiency of the bimetallic nano-enzyme is improved, the in-vivo retention time of the bimetallic nano-enzyme is prolonged, the bimetallic nano-enzyme has antibacterial, hemostatic, adhesion and anti-oxidation effects, the steady state of an extracellular microenvironment of the intervertebral disc is recovered, an extracellular matrix of intervertebral disc tissue is remodeled, and the effect of promoting the repair and regeneration of the intervertebral disc by the nano-enzyme is synergistically enhanced.

The invention provides a bacteriostatic agent aiming at yersinia enterocolitica and application, the bacteriostatic agent comprises a compound solution of lipoic acid and protocatechualdehyde, and when the lipoic acid and the protocatechualdehyde act synergistically, the minimum inhibitory concentrations to the yersinia enterocolitica are 0.625 mg / mL and 0.3125 mg / mL respectively. The yersinia enterocolitica biofilm is inhibited and / or removed through the lipoic acid and protocatechuic aldehyde compound liquid, and the problem that drugs for treating the yersinia enterocolitica infection are prone to drug resistance is solved.

The present invention relates an improved process for the preparation of piperonal (2), which is useful as an intermediate for the preparation of Tadalafil of formula (1).

The invention discloses construction and application of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum for integrally controlling a multi-component material basis of a traumatic injury bone-growing capsule, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and neochlorogenic acid, protocatechuic aldehyde, cryptochlorogenic acid, vanillic acid, rosmarinic acid, alkannic acid, berberine hydrochloride and salvianolic acid B are taken as reference substances; the fingerprint spectrum is successfully constructed by detecting different batches of traumatic injury bone-growing capsules. The method is simple and easy to implement, high in precision, good in reproducibility, accurate and reliable, at least eight components can be confirmed at a time under the same chromatographic condition, the detection efficiency is greatly improved, and time and cost are saved; quality monitoring can be carried out on a plurality of characteristic components of the traumatic injury bone-growing capsule accurately, rapidly and simultaneously, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect of the traumatic injury bone-growing capsule.

The invention relates to the technical field of intelligent hydrogel dressings, in particular to a preparation method and application of a chitosan-based hydrogel wound dressing, and the preparation method comprises the following steps: integrating sulfydryl chitosan (CS-SH), carbon nanotubes (CNTs) and protocatechualdehyde (PA), and forming the chitosan-based hydrogel wound dressing through a crosslinking effect. The chitosan-based hydrogel wound dressing disclosed by the invention has the characteristics of photo-thermal performance, excellent antibacterial activity, excellent electrical conductivity, sensing performance and the like. The hydrogel wound dressing has great potential in the aspects of infectious wound treatment and real-time body action monitoring, and a new design thought is provided for developing an intelligent wound management system with specific adaptability to patients.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

The application discloses a construction method of a UPLC characteristic spectrum of a radix-lithospermi and application thereof, 1) preparation of a control medicinal material reference solution; 2) preparation of a control reference solution: a certain amount of protocatechuic acid, protocatechuic aldehyde, ferulic acid, rosmarinic acid, caffeic acid tetramer and danshensu are taken to prepare a mixed solution by adding methanol, serving as the control reference solution; 3) preparation of a test solution; 4) determination: the control medicinal material reference solution, the control reference solution and the test solution are respectively taken and injected into a liquid chromatograph to determine characteristic spectra, wherein the characteristic spectra include at least 12 characteristic peaks; 5) qualitative analysis of a sample to be determined is carried out by using the characteristic spectra obtained in the above steps. The characteristic spectrum includes 12 characteristic peaks, can effectively distinguish radix-lithospermi (Xinjiang radix-lithospermi) and its pseudo products of different origins, and embodies the integrity of quality control of radix-lithospermi.

The present invention discloses a method for constructing a characteristic spectrum of a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction, comprising the following steps: preparing a reference solution, the reference solution comprising a control medicinal material reference solution and a reference substance reference solution, the reference substance reference solution comprising a protocatechuic acid reference substance, a protocatechuic aldehyde reference substance, a neochlorogenic acid reference substance, and a chlorogenic acid reference substance; taking a Corydalis guanzhong medicinal material, formula granules, decoction pieces or standard decoction to prepare a test solution; taking a preset amount of the reference solution and the test solution, injecting them into a liquid chromatograph, the liquid chromatograph using octadecylsilane bonded silica gel as a filler, methanol as mobile phase A, and phosphoric acid solution as mobile phase B for gradient elution to establish a characteristic spectrum. The present invention can provide a data basis for the quality control of Corydalis guanzhong medicinal materials, formula granules, decoction pieces or standard decoctions, and ensure the stability and controllability of product quality.

The invention relates to a high-efficiency hemostatic antibacterial gel dressing, which is prepared from the following raw materials: 4-carboxyphenylboronic acid-based chitosan, drug aldehyde, methacrylic acid esterified gelatin and tranexamic acid, wherein the 4-carboxyphenylboronic acid-based chitosan and the methacrylic acid esterified gelatin are dissolved in water to form a solution A; protocatechuic aldehyde and tranexamic acid are dissolved in water to form a solution B, the solution A and the solution B are rapidly and uniformly mixed to form a pre-injection solution before treatment, the pre-injection solution is injected to the surface of a wound surface so that the dressing can be cured and formed in situ, after forming, the dressing can effectively adhere to the skin and release drugs, rapid hemostasis and antibiosis are achieved, and the dressing can be degraded in about 10 days in a physiological solution environment; the gel dressing can be widely applied to treatment of different trauma wounds.

The application discloses a preparation method of an injectable self-healing hydrogel dressing, and relates to the technical field of medical materials, and particularly relates to a preparation method of an injectable self-healing hydrogel dressing: (1) mixing CuCl2.2H2O and protocatechuic aldehyde (PA) in deionized water, adjusting the pH to an alkaline environment, and obtaining a first solution containing a copper protocatechuic aldehyde complex (CuPA) through reaction; (2) dissolving methacrylated gelatin (GelMA) and an initiator in a buffer solution to obtain a second solution; (3) adding the first solution into the second solution and mixing uniformly, and then performing photocuring crosslinking to form the injectable self-healing hydrogel dressing. The application further discloses the injectable self-healing hydrogel dressing obtained through the above preparation method and application of the injectable self-healing hydrogel dressing in preparation of a diabetic infected wound dressing. The hydrogel dressing prepared by the application can realize multiple biological activities such as antibacterial activity, anti-inflammatory activity, antioxidant activity and pro-angiogenic activity at the same time, and has good blood compatibility and biological safety, and can be used for preparing a pro-infected diabetic wound healing dressing.

The invention provides a method for detecting HPLC (high performance liquid chromatography) characteristic chromatograms of senecio scandens medicinal materials, decoction pieces, standard decoction and formula granules, which comprises the following steps: pre-treating raw materials of a test sample to obtain liquid to be detected; the pretreatment mode comprises solvent dissolution and extraction; measuring the liquid to be measured by adopting HPLC (High Performance Liquid Chromatography) to obtain HPLC characteristic chromatograms of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules; the preparation method comprises the following steps: preparing a reference substance solution, namely dissolving neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol by adopting a solvent; obtaining a reference substance solution of the reference substance; the HPLC conditions are as follows: a chromatographic column is a phenyl column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.3% phosphoric acid aqueous solution, and gradient elution is carried out; according to the method, a high performance liquid chromatography method is adopted, and an acetonitrile-0. 3% phosphoric acid solution is selected as a mobile phase for gradient elution; the method comprises the following steps: taking neochlorogenic acid, protocatechuic aldehyde, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-coumaric acid, chlorogenic acid methyl ester, rutin, hyperoside, isoquercitrin, chlorogenic acid ethyl ester, isorhamnetin-3-O-galactoside, quercitrin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, quercetin and kaempferol as reference substances; the HPLC characteristic chromatogram method of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules is established, and more scientific technical means are provided for controlling the medicinal quality of the senecio scandens medicinal material, the decoction pieces, the standard decoction and the formula granules.

The invention discloses an HPLC specific chromatogram and component content detection method of a radix astragali-lepidii heart-strengthening capsule, and belongs to the technical field of quality control of traditional Chinese medicines. The establishment method of the HPLC characteristic chromatogram of the radix astragali-lepidium meyenii heart-strengthening capsule comprises the following steps: preparing a test solution from the content of the radix astragali-lepidium meyenii heart-strengthening capsule; the preparation method comprises the following steps: respectively weighing adenosine, guanosine, tanshinol, protocatechuic acid, protocatechuic aldehyde, catechin, 4-methoxysalicylaldehyde, calycosin glucoside, narirutin, hesperidin, rosmarinic acid, alkannic acid, salvianolic acid B, apigenin and formononetin, and preparing a mixed reference solution; and carrying out HPLC detection analysis on the test solution and the mixed reference substance solution to obtain the HPLC characteristic chromatogram of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule. The method has the advantages of strong specificity, high precision, good stability and good repeatability, can realize characteristic spectrum identification and multi-component content control of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule at the same time, and provides a reliable basis for production quality supervision and clinical medication safety of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule.

The present application relates to the technical field of edible and medicinal fungus fermentation, and discloses a fermentation method of Phellinus baumii with high Hispidin yield and application.The fermentation method of Phellinus baumii in the present application comprises inoculating Phellinus baumii strains into a culture medium containing copper ion compounds, and then fermenting to obtain the product.The mycelium of Phellinus baumii obtained by the fermentation method of the present application is rich in Hispidin, and can be widely used in the preparation of related drugs and health foods for liver protection, anti-tumor, anti-virus, oxidation resistance, uric acid reduction, etc.In addition, the present application also discloses a high-performance liquid chromatography detection method for analyzing the content of Hispidin, which can effectively analyze the content of Hispidin and its precursor substances (such as protocatechuic acid, protocatechuic aldehyde, coffee acid, and aspidin) in the fermentation product of Phellinus baumii, its derivative product, and the mycelium of Phellinus baumii, and is helpful to improve the accuracy of Hispidin detection.

The present invention discloses a method for establishing a UPLC fingerprint of Weimaining capsules, comprising the following steps: (1) preparing a test solution; (2) respectively examining the precision of an ultra-high performance liquid chromatograph, the repeatability of a test solution preparation method, and the stability of the test solution; (3) subjecting the test solution to gradient elution in a chromatographic column, and scanning the test solution using an ultra-high performance liquid chromatograph to obtain a chromatogram of the test solution; (4) identifying the common peak components of the chromatogram using reference substances such as gallic acid, protocatechuic acid, protocatechuic aldehyde, proanthocyanidin B2, epicatechin, and rutin. (5) establishing a fingerprint. The ultra-high performance liquid chromatograph used in the present invention has high precision, and the test solution preparation method provided has good repeatability. The fingerprint established based on the above two points has strong stability, and the speed of establishing the fingerprint is fast, the sensitivity is high, and the separation of the chromatographic peaks is good.

The invention provides enzyme cross-linked polyvinyl protocatechuic aldehyde hydrogel as well as a preparation method and application thereof. The hydrogel is prepared from a cross-linked substance formed by a matrix and the balance of water or a phosphate buffer solution, the matrix is a polyvinyl protocatechuic aldehyde solution, and a cross-linking agent of the matrix is an enzyme catalysis system solution of horseradish peroxidase / hydrogen peroxide combination; the hydrogel has photo-thermal responsiveness to near infrared rays, and preferably, the hydrogel has photo-thermal responsiveness to near infrared rays with the wavelength of 808 nm. The hydrogel material is easy to prepare, green in synthesis and good in biological safety, the advantages of polyvinyl alcohol and protocatechualdehyde are combined, polyvinyl protocatechualdehyde is synthesized, and photo-thermal response type hydrogel is rapidly formed through catalysis of horseradish peroxidase / hydrogen peroxide. Extra photo-thermal corresponding materials do not need to be added, and the effects of rapidly killing bacteria at the wound part and promoting the wound to heal can be achieved by combining the characteristics of oxidation resistance, bacteria resistance and the like of the hydrogel and adopting a photo-thermal therapy method.

This invention relates to the field of biomedical technology and discloses a method for preparing and applying copper-manganese coordinated taurine-Schiff base nanopolymers. The preparation method includes: dissolving taurine and an equimolar amount of sodium hydroxide in anhydrous ethanol, heating under reflux, and adding protocatechuic aldehyde dissolved in anhydrous ethanol dropwise while stirring; heating under reflux and stirring; a yellow solid precipitates from the reaction solution, which is then transferred to a refrigerator to further precipitate the solid, and collected to obtain taurine-Schiff base; dissolving copper chloride and manganese chloride in an aqueous solution of polyvinylpyrrolidone, adding taurine-Schiff base while stirring, adjusting the pH of the reaction solution to alkaline with an alkaline solution, stirring, and collecting to obtain copper-manganese coordinated taurine-Schiff base nanopolymers; ultrasonically dispersing the nanopolymer in ultrapure water, adding an anti-angiogenic drug, stirring, and collecting to obtain drug-loaded copper-manganese coordinated taurine-Schiff base nanopolymers. This invention achieves effective local treatment and immune system activation, while improving treatment safety.

The invention relates to multifunctional composite hydrogel and a preparation method and application thereof.The multifunctional composite hydrogel is prepared from raw materials including the following components through a cross-linking reaction: a conductive and photo-thermal functional unit which is a carboxylated single-walled carbon nanotube grafted by epsilon-poly-L-lysine and polyethylene glycol diamine, recording as epsilon-PL-CNT-PEG (Polyethylene Glycol); the dynamic cross-linked network unit comprises hydrazide chondroitin sulfate and a protocatechuic aldehyde-iron complex, the hydrazide chondroitin sulfate is marked as CS-ADH, and the protocatechuic aldehyde-iron complex is marked as PA at Fe. The multifunctional composite hydrogel disclosed by the invention can realize electric conduction, photo-thermal antibiosis, oxidation resistance, self-healing, adhesion and pH response degradation in the same system.

This invention belongs to the field of immobilized enzyme preparation technology. More specifically, it relates to a biological enzyme immobilization medium that can be prepared in various forms and its preparation method. This invention provides a medium for immobilizing enzymes, comprising silk fibroin and protocatechuic aldehyde; the mass ratio of silk fibroin to protocatechuic aldehyde is (10-30):(0.06906-172.65). The biological enzyme immobilization medium of this invention has simple components, a simple preparation method, and low cost. It also effectively solves the problem of reduced enzyme activity caused by existing immobilization media. It can immobilize different types of enzymes, improving the enzyme's cycling stability and temperature tolerance. Furthermore, this immobilization medium can be prepared in various forms, such as coatings, films, hydrogels, and microspheres, and can stably bind to different material surfaces to meet the needs of different application scenarios. In addition, the biological enzyme immobilization medium of this invention has good biocompatibility and has excellent application prospects and value.

The application discloses a method for extracting phenolic active substances from Phellinus baumii fruiting bodies by adopting a series extraction and purification method and application of the phenolic active substances in uric acid regulation, and belongs to the technical field of deep processing of edible fungi. The method is as follows: after the Phellinus baumii fruiting bodies are soaked in an acetone solution, a crude acetone extract is obtained by using a multi-frequency ultrasonic device, then the crude acetone extract is dissolved in ethanol, and a HPD-826 macroporous resin filler and a LX-17 macroporous resin filler are connected in series to carry out adsorption and elution, so that a pure Phellinus baumii polyphenol extract is obtained. The polyphenol content of the phenolic active substances prepared from the Phellinus baumii fruiting bodies is as high as 80% or more, main active substances are protocatechuic acid, protocatechuic aldehyde, coffee acid, p-coumaric acid, dihydroquercetin, Hispidin and the like, and the phenolic active substances can be prepared into medicines for regulating intestinal flora structure and uric acid metabolism.

The present application relates to ascorbate peroxidase and its coding gene and application. The ascorbate peroxidase of Fritillariae Pallidicaulis Herb is disclosed for the first time, which has the ability of synthesizing protocatechuic aldehyde and can catalyze p-hydroxybenzaldehyde to generate protocatechuic aldehyde. The present application also discloses polynucleotide coding the ascorbate peroxidase, vector and host cell expressing the ascorbate peroxidase, and method for producing protocatechuic aldehyde.

The invention discloses nano composite hydrogel capable of releasing NO as well as a preparation method and application of the nano composite hydrogel. The nano composite hydrogel capable of releasing NO comprises NO precursor nanoparticles loaded in a fucoidan matrix, the NO precursor nano particles are obtained by self-assembly of protocatechuic aldehyde and S-nitroso glutathione. The preparation method of the nano-composite hydrogel capable of releasing NO comprises the following steps: mixing a protocatechuic aldehyde solution and an S-nitrosoglutathione solution, and reacting at room temperature in a dark place to obtain an aqueous solution containing NO precursor nanoparticles; the preparation method comprises the following steps: mixing an aqueous solution containing NO precursor nanoparticles with a fucoidan solution, and standing at 4 DEG C to form gel; the nano-composite hydrogel disclosed by the invention can realize controllable release of NO under ultrasonic stimulation, has excellent antibacterial, free radical scavenging and moist wound adhesion characteristics, and can be applied to repair of infected wounds.