68 results about "Protocatechuic aldehyde" patented technology

The invention relates to a synthesis method of D,L-danshensu isopropyl ester. The method comprises taking protocatechuic aldehyde as an initial raw material, protecting phenolic hydroxyl by benzyl, carrying out Darzens epoxidation, followed by carrying out catalytic reduction with palladium catalyst/hydrogen or Raney nickel/hydrogen, and thus obtaining the D,L-danshensu isopropyl ester. The purity of the product synthesized by the method can reach 98%, and the yield can reach 55%. Moreover, the synthesis method has the advantages of simple and easily obtained raw materials, short routes and high yield, and is suitable for large-scale industrialized production.

The invention is concerned with a kind of extract of total ketone of salviae miltiorrhizae and total phenolic acid and its produce method form radix Salviae Miltiorrhizae. The extract of total ketone of salviae miltiorrhizae has cryptotanshinone, tanshinone I, tanshinone IIA, methyl Tanshinon, dihydrotanshinon I and ramification. The extract of total phenolic acid has salvianolic acid A, salvianolic acid B, protocatechuic aldehyde and ramification. The extract can be got form one or arbitrary compound of extraction with solvent method, macroporous resin method, column chromatography and liquid-liquid counter-current chromatography. The summation of the content to each total ketone of salviae miltiorrhizae is 20 to 100 percnte (w/w) of the extract of total ketone of salviae miltiorrhizae, the contene of cryptotanshinone, tanshinone I and tanshinone IIA is 5 to 100 percent (w/w) of whole content of total ketone of salviae miltiorrhizae. The summation of the content to each total phenolic acid is 5 to 100 percent (w/w) of the extract of the radix salviae miltiorrhizae total phenolic acid. The content of salvianolic acid B is the 5 to 100 percent (w/w) of the whole salvianolic acid.

The present invention relates to Chinese medicine quality detecting technology, and is especially the quality detection method for compound preparation of red sage and notoginseng. HPLC-DAD process is adopted to measure the contents of protocatechuic aldehyde, salvianolic acid B, cryptotanshinone, neotanshinone IIA, arasaponin R1, ginsenoside Rg1 and ginsenoside Rb1 in compound red sage preparation simultaneously. The process is simple, precise, repeatable and reliable, and may be used in the quality control of compound red sage preparation.

Disclosed is a medicinal composition for treating senile dementia, wherein 1g of the dried active sites comprises tanshinone IIA 1.79-9.01mg, salvianolic acid B 35.87-225.23mg, danshensu 4.93-45.05mg, protocatechuic aldehyde 0.10-4.50mg, and notoginseng saponin R 13.14-22.50mg.

The invention discloses a method for establishing an HPLC fingerprint spectrum of a Zhuang medicinal material Blumea riparia (Bl.) DC. By adopting a high-performance liquid chromatography, and using main ingredients such as protocatechuic acid and protocatechuic aldehyde of Blumea riparia (Bl.) DC as reference substances, a fingerprint spectrum of a common pattern of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material is obtained. The fingerprint spectrum has 13 chromatographic peaks, can fully reflect the chemical compositions of the Blumea riparia (Bl.) DC medicinal material, and is rich in information amount, the method has good reproducibility, a more powerful theoretical basis is provided for controlling the quality of the medicinal material and identifying the advantages and disadvantages of the medicinal material, the quality control and the level of true and false identification of the Zhuang medicine Blumea riparia (Bl.) DC medicinal material are improved, and the authenticity and the advantages and disadvantages of the Blumea riparia (Bl.) DC can be quickly and accurately identified.

The invention discloses a structural general formula of a compound LQC-T and the synthesis and the application of the compound LQC-T. Pharmacological experiments prove that the compound has an obvious effect of promoting new vessels of chick chorioallantoic membranes to grow; a compound LQC-T4 has an obvious medicinal effect of treating ischemic brain injury stroke and the sequela of the ischemic brain injury stroke; the maximal LQC-T4 single-day dose of mice is 5,400mg/kg; and the compound is extremely high in safety and can be used for preparing a medicine for treating the ischemic brain injury stroke and the sequela of the ischemic brain injury stroke after toxic responses do not occur after continuous observation within 1 to 4 days. According to the structural formula of the compound LQC-T, R is protocatechuic acid, protocatechuic aldehyde, vanillic acid, gallic acid, caffeic acid, ferulic acid and other aromatic organic acids or phenols or structural analogues of the organic acids or phenols.

The invention discloses a method for detecting the content of water-soluble ingredients in a salvia miltiorrhiza medicinal material and an application of the method. The method is an ultra-performance liquid chromatography method by using reference substances of tanshinol, protocatechualdehyde, rosmarinic acid, salvianolic acid B and salvianolic acid A as reference and 0.026% phosphoric acid aqueous solution-acetonitrile in a ratio being (85-10):(15-90) as a moving phase for gradient elution. The method for detecting the content of water-soluble ingredients in a salvia miltiorrhiza medicinal material can be used for simultaneously detecting the content of multiple water-soluble ingredients in a salvia miltiorrhiza medicinal material, and can be applied to detection of salvia miltiorrhiza medicinal material fingerprint spectrum. The method has a good effect for separating water-soluble ingredients in the salvia miltiorrhiza medicinal material, and has the characteristics of quickness, accuracy, high reproducibility and high recovery rate, and has strong specificity, high precision, good repeatability, high stability and accurate measurement result. By applying the method, multiple peaks of the fingerprint spectrum can be detected, and reflected information is relatively complete; and the peaks have good shapes, can be easily identified, have high similarity, and are accurate and reliable.

A freeze-dried powder injection for treating cardiovascular and cerebrovascular diseases is prepared from red sage root and safflower through immersing safflower in worm water, decocting red sage root, mixing the immersing water with decoction, concentrating, depositing in alcohol, examining and freeze drying. Its test method is to measure the contents of danshinolic acid, danshensu sodium, protocatechuic aldehyde, and safflor yellow.

A Chinese medicine 'Xuemingmu' for treating eyeground hemorrhage is disclosed. Its quality test method features that the scutelloside, emodin, chrysophanol and protocatechuic aldehyde are qualitatively discriminated, and the content of danshensu is measured by high-effect liquid-phase chromatography.

The invention relates to a natural pharmaceutical composition as well as a traditional Chinese medicine composition containing the same and application of the natural pharmaceutical composition. The natural composition comprises the following raw materials in weight ratio: 40 to 200 parts of paeoniflorin, 1 to 20 parts of ferulic acid, 10 to 50 parts of hydroxysafflor yellow and 1 to 20 parts of protocatechuic aldehyde. The traditional Chinese medicine composition is a composition containing the natural pharmaceutical. The natural pharmaceutical composition and the traditional Chinese medicinecomposition have anti-aGvHD and anti-fungal infection effects, and are of great significance to improve the success rate of transplantation, improve the prognosis of patients, and increase the long-term survival rate of patients with hematological diseases, recipients of allogeneic hematopoietic stem cells and patients with fungal sepsis.

The invention belongs to field of medicines and relates to a method for preparing high-purity protocatechualdehyde. The preparation method comprises the following steps: 1, preparing salvia miltiorrhiza extract by alkaline extraction and alcohol precipitation; 2, removing salvia miltiorrhiza components with relatively weak polarity by chromatography; 3, separating protocatechualdehyde by chromatography; 4, removing acid and decoloring; 5, refining and purifying; and 6, drying.

The invention relates to a medicinal composition for treating renal diseases and the pharmaceutical use of the medicinal composition. The composition contains rhein, physcion, emodin glucoside, chrysophanol glucoside, salvianic acid, salvianolic acid B, hydroxysafflor yellow A, astragaloside, astragalus polysaccharide, salvianolic acid A, calycosin-7-glucoside, physcion glucoside, rosmarinic acid, carthamin yellow A, salvianolic acid D, salvianolic acid H, protocatechuic aldehyde, caffeic acid and gallic acid, wherein the chrysophanol glucoside is chrysophanol monoglucoside, the emodin glucoside is emodin monoglucoside, and the physcion glucoside is physcion monoglucoside. The composition can also contain C11H9N6O4, C17H14O4, C21H20O9, C19H32O12, C30H36O15 and C30H44O15. The composition is a preparation in a solid form, a liquid form, a gas form or a semisolid form, and a great number of experiments show that the composition can be used for treating renal diseases, renal failure, nephrotic syndromes, cardiovascular and cerebrovascular diseases, neoplastic diseases and the like.

The invention provides a detection method of phenolic acid compounds in a compound radix salviae miltiorrhizae tablet, and the detection method comprises the following steps: preparing a standard solution, a reference solution and a to-be-tested product solution, passing through a high performance liquid chromatography for detection; then calculating a relative correction factor of the phenolic acid compounds relative to salvianic acid A sodium; and using a formula IV shown in the specification too obtain the phenolic acid compound content in the compound radix salviae miltiorrhizae tablet. According to the detection method, by detection of one compound (salvianic acid A sodium), simultaneous determination of multiple compounds (salvianic acid A, protocatechuic aldehyde, rosmarinic acid, lithospermic acid and salvianolic acid B) can be realized, and the detection method is simple, rapid, accurate, good in universality, labor, and material and financial resource saving, and can more fully reflect the quality of the compound radix salviae miltiorrhizae tablet.

The invention relates to a method for preparing protocatechuic aldehyde from vanillin. The method comprises the following steps: (1) adding vanillin and a solvent into a reactor, wherein the use amount of the solvent is 1-10 ml/g vanillin; fully stirring, and adding a demethylation reagent, wherein the molar ratio of the vanillin to the demethylation reagent is 1:(0.6-1.6); (2) maintaining the reactor temperature at 0-10 DEG C, and adding pyridine, wherein the use amount of the pyridine is 0.5-2.6 ml/g vanillin; (3) raising the reaction system temperature to 30-90 DEG C, and reacting for 2-16 hours; (4) adding 12wt% diluted hydrochloric acid under stirring condition for acidifying to pH 2-4, continuously stirring for 0.5-1.0 hour, and stopping reaction; (5) extracting the reaction liquid with dichloromethane; and (6) then extracting the raffinate phase with ethyl acetate, drying, distilling and purifying to obtain protocatechuic aldehyde. In the method provided by the invention, an environmentally-friendly carbonate solvent is used to substitute for the dichloromethane solvent, the reaction temperature is increased and the reaction time is greatly shortened; and when ZnCl2 is used as the demethylation reagent, the equipment corrosion problem caused by the use of AlCl3 can be reduced.

The invention relates to the discovering of the action targets of traditional Chinese medicine salviae miltiorrhizae and widening of clinical application range, in particular to discovering of the new action targets of phenolic acid compounds in the salviae miltiorrhizae and new clinical application of the phenolic acid compounds. The phenolic acid compounds include similar-structured compounds such as protocatechuic aldehyde, tanshinol, caffeic acid, alkannic acid, salvianolic acid A, salvianolic acid B, salvianolic acid C and salvianolic acid D, the derivatives of the similar-structured compounds and the pharmaceutically-acceptable salt-forming compounds of the similar-structured compounds. In vitro cell experiments show that the compounds act on the orphan receptor GPR35 and current researches show that the orphan receptor GPR35 is related to diseases such as cardiac failure, hypertension, coronary heart disease, metabolic syndrome, asthma, pain, inflammation and cancer, so that the clinical application range of the compounds can be widened. In addition, the fact that the compounds have a double-target effect is discovered, and certain guidance is provided for the new clinical application.

The invention provides a method for determining components of common wedgelet fern herb by adopting quantitative analysis multi-components by a single marker. The method is characterized in that the quantitative analysis multi-components by the single marker is carried out by taking protocatechuic acid which is cheap and easy to obtain as an internal reference object, and relative correction factors between protocatechuic acid, and protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb are established; the content of protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb is calculated through the correction factors; and a liquid chromatography is used for determining. The detection method is high in practicability and simple to operate and the cost is saved; and the content of four components including protocatechuic acid, protocatechuic aldehyde, orientoside and vitexin in common wedgelet fern herb can be effectively detected.

The present invention relates to Chinese medicine preparation, and is especially freeze dried injection powder preparation for treating coronary heart disease and its preparation process. The freeze dried injection powder preparation is prepared with red sage and Chuanxiong rhizome as main material, and through the steps of water extraction, precipitating to eliminate impurity, refining with macroporous resin, filtering, compounding the refined extract and freeze drying excipient with injection water to form solution, refining, packing and low temperature freeze drying. Each injection contains protocatechuic aldehyde 10-15mg, total phenolic acid 300-350mg and ligustrazine 10-15mg. The present invention has the advantages of simple preparation process, low cost, high stability, obvious curative effect, etc.

The invention provides a method for determining the content of active ingredients in Chinese honeylocust fruit, which adopts ultra-high performance liquid chromatography-mass spectrometry. The content of L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopoletin, liquiritigenin, apigenin, luteolin, eriodictyol, citrus aurantium, fustin, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin and isoorientin in the Chinese honeylocust fruit can be rapidly detected. The method provided by the invention has the advantages of simple operation, high sensitivity, fast analysis speed and strong specificity, and can be used for quality control of Chinese honeylocust fruit.

The invention discloses a purpose of 3,4-dihydroxy phenyl substituted phenol for preparing an ALDH2 activating agent and medicine for preventing and treating cardio-cerebral ischemia injury. The invention provides a purpose of a 3,4-dihydroxy phenyl substituted phenol compound including but not limited to protocatechuic acid, tanshinol, danshinolic acid B and danshinolic acid A for preparing the ALDH2 activating agent, and further provides a purpose of the 3,4-dihydroxy phenyl substituted phenol compound for preparing medicine for preventing and treating cardio-cerebral ischemia injury; a medicine composition for preventing and controlling myocardial infarction or cerebral apoplexy by 3,4-dihydroxy phenyl substituted phenol or pharmaceutical salts of the 3,4-dihydroxy phenyl substituted phenol. The 3,4-dihydroxy phenyl substituted phenol or pharmaceutical salts of the 3,4-dihydroxy phenyl substituted phenol can be used for preparing medicine for preventing and controlling diseases relevant to ALDH2.

The present invention relates to the application of substituted derivative of protocatechuic aldehyde, ester derivative of protocatechuic aldehyde, acetal derivative of protocatechuic aldehyde, amide derivative of protocatechuic aldehyde, and Schiff base derivative of protocatechuic aldehyde in treating hepatitis B and other viral infectious diseases; and the said medicine compound or their composition may be used through combination with antiviral neucleoside analog medicine in treating hepatitis B and other viral infectious diseases.

The invention provides prepared cibotium barometz tuber effective parts and a preparation method thereof. The preparation method comprises the following steps of taking 40g of prepared cibotium barometz tuber medicinal material fine powder, adding 800mL of methanol-water in the volume ratio being 70 to 30, performing reflux extraction twice, wherein the reflux extraction time each time is 2h, performing filtration, and taking filtrate for standby application; additionally taking 3g of activated DM2 type resins, and performing loading into a column; taking 15mL of a cibotium barometz tuber extracting solution, conveying samples to a detection instrument, performing adsorption for 4h, performing eluting with 45mL of methanol-water in the volume ratio being 20 to 80, collecting an eluent, andperforming evaporation to dryness so as to obtain the prepared cibotium barometz tuber effective parts. According to the cibotium barometz tuber effective parts prepared by the method, the kojic acidcontent in percentage by mass is more than 0.4%, the 5-HMF content in percentage by mass is more than 13.5%, the protocatechuic acid content in percentage by mass is more than 0.6%, and the protocatechuic aldehyde content in percentage by mass is more than 0.5%. The preparation method is simple and environmental-friendly and is suitable for large-scale industrial production.

The invention relates to a determination method of a capsule fingerprint spectrum for dredging collaterals and reducing phlegm, which comprises the following steps: (1) test solution prepartion: taking capsule contents for dredging collaterals and reducing phlegm, adding methanol, carrying out ultrasonic extraction, filtering, and taking the subsequent filtrate to obtain the test solution; (2) preparation of reference substance solutions: taking tauroursodeoxycholic acid, gastrodin, sodium tanshinol, tanshinone IIA, salvianolic acid B, protocatechuic aldehyde, ginsenoside Rb1, ginsenoside Rg1,notoginsenoside R1, rheum emodin and rheinic acid reference substances which are dried under reduced pressure to constant weight, and respectively adding methanol to prepare the reference substance solutions; (3) determination: precisely measuring the test solution and the reference solution respectively, injecting the solutions into a high performance liquid chromatograph for determination, andrecording chromatograms; and (4) performing comparison according to the chromatogram and the standard comparison fingerprint, and determining that the product is qualified if the chromatogram and thestandard comparison fingerprint are consistent.