Licorice medicinal materials fingerprint establishment method and its standard fingerprint

A standard fingerprint and fingerprint technology, applied in the field of drug analysis, can solve the problems of not being able to systematically and completely reflect the internal quality of licorice, and achieve the effects of easy realization of chromatographic conditions, simple production, good stability and reproducibility

Inactive Publication Date: 2008-07-09
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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Problems solved by technology

The quality control of licorice medicinal materials in "Chinese Pharmacopoeia" is limited to the determination of gl

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  • Licorice medicinal materials fingerprint establishment method and its standard fingerprint

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[0017] Preferred embodiments of the present invention are as follows:

[0018] (1) Instruments and medicines: Shimadzu LC10A high-performance liquid chromatography was used; 16 batches of licorice medicinal materials were purchased from Chinese herbal medicine stores; other reagents were chromatographically pure or analytically pure.

[0019] (2) Preparation of the test solution: Accurately weigh 10 g of medicinal material powder, place it in a volumetric flask, add 600 mL of 70% ethanol, oscillate ultrasonically for 45 min, and cool. The supernatant was filtered with a 0.45 μm microporous membrane, and the filtrate was used as the test solution.

[0020] (3) HPLC analysis: the chromatographic separation column packing is 5μm Kromasil-C18, 250×4.6mm; the mobile phase is 0.8% acetic acid aqueous solution-acetonitrile; the gradient elution method is adopted: 0min→25min→50min→70min→95min, Acetonitrile 11%→28%→45%→60%→78%; flow rate 1.0mL / min; detection wavelength 254nm; column t...

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Abstract

The invention provides a method for establishing an HPLC fingerprint spectrum of Radix Glycyrrhizae and a standard fingerprint spectrum thereof. The method comprises the following steps of: firstly adding Radix Glycyrrhizae powder into a 70% ethanol aqueous solution, ultrasound-extracting, filtering the supernate with a microporous membrane to obtain a filtrate as the test solution; then carrying out HPLC analysis, wherein the chromatographic column is Kromasil-C18 (5 mum, 250*4.6 mm), the mobile phase is 0.8% acetic acid solution-acetonitrile, the gradual eluting mode is adopted, the detection wavelength is 254 nm and the column temperature is 30 DEG C; and extracting the test solution, injecting into the HPLC to obtain the HPLC fingerprint spectrum of Radix Glycyrrhizae. The standard fingerprint spectrum of Radix Glycyrrhizae can be obtained by comparing HPLC fingerprint spectra of 16 batches of Radix Glycyrrhizae materials and determining the common fingerprint characteristics. The standard fingerprint spectrum can be used for effectively monitoring the quality of Radix Glycyrrhizae.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing fingerprints of high performance liquid chromatography (HPLC) medicinal materials of licorice, and a standard fingerprint of medicinal materials of licorice obtained by the method. The invention belongs to the technical field of pharmaceutical analysis. Background technique [0002] Fingerprint of traditional Chinese medicine specifically refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography of traditional Chinese medicine samples. At present, fingerprints have become one of the most effective means to identify the varieties of traditional Chinese medicines and evaluate the quality of traditional Chinese medicines recognized at home and abroad. In the "FDA Guidelines on Plant Products" ...

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Application Information

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IPC IPC(8): G01N30/86G01N30/06G01N30/02G01N33/15
Inventor 周考文李凤桐史晓南
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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