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Method for establishing quantitative fingerprint spectrum of standard fructus evodiae decoction

A fingerprint and Evodia technology, which is applied in the field of establishing the quantitative fingerprint of Evodia standard decoction, can solve the problems of providing reference for the quality control of difficult Evodia formula granules, unable to meet the quality control requirements of Evodia, analysis time and efficiency constraints, etc., and achieve stability. and good reproducibility, easy realization of chromatographic conditions, and reduction of detection costs.

Pending Publication Date: 2022-07-05
赣江中药创新中心
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Problems solved by technology

[0004] At present, the National Pharmacopoeia Commission has published the national standard of Evodia rutaecarpa formula granules. The source of the medicinal material is Evodia rutaecarpa. The quality control methods stipulated in the standard include two items: characteristic map and content determination. Two methods for quality control of Evodia rutaecarpa formula granules, analysis time and efficiency are restricted
In addition, Zhang Hongmei et al. established the UPLC quantitative fingerprint of Evodia rutaecarpa, and evaluated the quality of Evodia rutaecarpa from different origins through similarity and determination of limonin, evodiamine and evodiamine content [Zhang Hongmei. Chinese Journal of Experimental Formulas ,2014,20(17):69-73.], but the matching in the paper identified 9 common peaks in the fingerprint, and did not identify the 9 peaks
Qi Huawen et al. used HPLC technology to establish a quantitative fingerprint method for medicinal materials of Evodia rutaecarpa, which matched 13 common chromatographic peaks and qualitatively identified them by mass spectrometry, and determined the content of 4 components, evodiamine, evodiamine, dehydroevodiamine and narcisin. Determination, but the analysis time of the HPLC technology used is too long, which affects the analysis time and efficiency [Qi Huawen. Chinese Herbal Medicine, 2021,52(14):4341-4347.]
[0005] In the existing standards and literature, the standard decoction of Evodia rutaecarpa (Shihu) lacks effective quality control methods, and it is difficult to provide a reference for the quality control of Evodia rutaecarpa (Shihu) formula granules
The national standard method of Evodia rutaecarpa formula granules with similar bases has problems such as long analysis time, low analysis efficiency, and insufficient quality control methods, and the quality control methods reported in the literature are mostly alcohol extraction, and the analysis time is long, which cannot meet the requirements of Evodia rutaecarpa ( Shihu) standard decoction quality control requirements

Method used

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  • Method for establishing quantitative fingerprint spectrum of standard fructus evodiae decoction
  • Method for establishing quantitative fingerprint spectrum of standard fructus evodiae decoction
  • Method for establishing quantitative fingerprint spectrum of standard fructus evodiae decoction

Examples

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example 1

[0042] Example 1: Establishment of UPLC characteristic map method for Evodia (Shihu) standard decoction

[0043] 1.1 Instruments, reagents, reagents and sources

[0044] Instruments: Waters Acquity I Class ultra-performance liquid chromatograph; Agilent Infinity II1290-6545Q-TOF LC-MS Agilent ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometer; ML204T / 02 1 / 00000 analytical balance; XSE105 ten 1 / 10,000 analytical balance; KQ-500DV ultrasonic instrument; Waters Acquity UPLC BEH ShieldRP18 (2.1×100mm, 1.7μm) chromatographic column and pre-column; SOrvall ST8 high-speed centrifuge;

[0045] Reagents: acetonitrile (Thermo) and formic acid (Thermo) are mass spectrometry grade; phosphoric acid (Aladdin) and acetonitrile (Fisher) are chromatography grade; methanol (General-Reagent) is analytical grade; ultrapure water (Milli-QIQ 7000);

[0046] Reagents: chlorogenic acid (batch number: 110753-202018, specification: 20mg, purity: 96.1%), hypericin (batc...

example 2

[0083] Example 2: Establishment of the content determination method of Evodia (Shihu) standard decoction

[0084] 1.1 Instruments, reagents, reagents and sources

[0085] Instrument: Waters Acquity I Class ultra-high performance liquid chromatograph; ML204T / 02 1 / 10,000 analytical balance; XSE105 1 / 100,000 analytical balance; KQ~500DV ultrasonic instrument; Waters Acquity UPLC BEH ShieldRP18 (2.1×100mm, 1.7μm ) chromatographic column and pre-column; SOrvall ST8 high-speed centrifuge;

[0086] Reagents: formic acid (Macklin) and acetonitrile (Fisher) are chromatographic grade; methanol (Energy Chemical) is analytical grade; ultrapure water;

[0087] Reagents: limonin (batch number 110800-201707, specification 20mg, purity 97.9%) was purchased from China National Institute for Food and Drug Control; Hospital; Evodia (batch number 110801-2021109, specification 20mg, purity 99. Macklin 3%) was purchased from China National Institute for Food and Drug Control.

[0088] The source...

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Abstract

The invention discloses a method for establishing a quantitative fingerprint spectrum of a fructus evodiae standard decoction, and relates to the field of establishment of fingerprint spectrums.The method comprises the following steps that 1, a reference substance solution is prepared; (2) preparing a test solution; (3) carrying out ultra-high performance liquid chromatography analysis; and (4) establishing a quantitative fingerprint, introducing multiple batches of liquid chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, matching common peaks, generating a control characteristic spectrum, selecting reference peaks, calculating the relative retention time of each characteristic peak, and determining the content of three components, namely limonin, evodiamine and rutaecarpin. And establishing a quantitative fingerprint spectrum of the fructus evodiae standard decoction. According to the method, the characteristic chromatogram and the content of the fructus evodiae standard decoction can be detected at the same time through one-time analysis, the detection time can be effectively shortened, the working efficiency is improved, and a guarantee is provided for quality and consistency evaluation of fructus evodiae formula granules.

Description

technical field [0001] The invention relates to the field of establishment of fingerprints, in particular to a method for establishing quantitative fingerprints of Evodia Fructus standard decoction. Background technique [0002] Evodia is Rutaceae Euodia rutaecarpa (Juss.) Benth., Shihu E.rutaecarpa (Juss.) Benth.var.officinalis (Dode) Huang or Evodia rutaecarpa (Juss.) Benth.var.bodinieri ( The dried and near-ripe fruit of Dode) Huang has the functions of dispelling cold and relieving pain, reducing inversion and relieving vomiting, and helping yang and relieving diarrhea. Acid, five more diarrhea and so on. Modern studies have shown that Evodia mainly contains alkaloids, bitter substances, volatile oils and flavonoids, among which evodial and evodial are the main active components. Modern pharmacological studies have shown that it has analgesic, anti-inflammatory, anti-ulcer, antibacterial, anti-tumor and protective effects on the cardiovascular system [Liu Li. Chinese H...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/8631
Inventor 梁鑫淼薛倩倩金红利陆绍铭张旗刘艳芳许妍
Owner 赣江中药创新中心
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