166 results about "Chemogenetics" patented technology

The invention relates to the technical field of molecular markers, in particular to KASP primers of 112 carya illinoensis varieties, an SNP molecular marker, a fingerprint spectrum and application of the KASP primers and the SNP molecular marker. By adopting the technical scheme provided by the invention, the carya illinoensis fingerprint spectrum can be drawn, is used for accurate identification of carya illinoensis varieties, and has important significance on germplasm resource identification and molecular marker breeding of carya illinoensis.

The invention discloses a method for identifying dracocephalum heterophyllum benth in different drying modes, which comprises the following steps: S1, drying dracocephalum heterophyllum benth, including natural drying in the shade, hot air drying and vacuum freeze drying; s2, feeding the dried sample in a headspace mode; s3, a GC-IMS instrument is used for analysis; and S4, carrying out qualitative and quantitative analysis on the detected volatile components through VOCal software, and constructing a GC-IMS spectrogram and a fingerprint spectrum of the volatile components. Through GC-IMS technology and VOCal software analysis, dracocephalum heterophyllum benth subjected to natural shade drying, hot air drying and vacuum freeze drying treatment can be distinguished precisely with high sensitivity, and a reliable technical means is provided for quality control of medicinal materials.

The invention discloses a breast lump removing capsule / granule fingerprint construction method and a fingerprint thereof, and belongs to the field of traditional Chinese medicine quality standard research and application. Respectively injecting the test solution and the single reference solution into a high performance liquid chromatograph, carrying out gradient elution, and carrying out chromatographic analysis to obtain various chromatograms; performing mass spectrometry on the test solution to obtain a mass spectrometry result; taking chromatographic peaks existing in the chromatogram of the test solution as common peaks, and identifying chemical components of the chromatographic peaks in the breast lump removing capsule / particle fingerprint spectrum by combining the chromatogram of the single reference substance solution and the mass spectrum result of the test solution, so as to obtain the breast lump removing capsule / particle fingerprint spectrum. The detection method for the breast lump removing capsule / particle fingerprint spectrum has the advantages of being simple, convenient, stable, high in precision and good in reproducibility, the quality of the breast lump removing capsule / particle can be evaluated more comprehensively and scientifically, and the quality and the curative effect of a product are guaranteed.

The invention belongs to the field of wastewater treatment and industrial automatic control, and particularly relates to an intelligent dosing control method based on water quality online monitoring. The method comprises the following steps: capturing a water body spectrum fingerprint spectrum by using a full-spectrum scanning module to obtain a characteristic signal sequence; a chemical informatics molecular algorithm is introduced for preprocessing and feature enhancement, and molecular fingerprint information is recognized; a convolutional neural network model is constructed to decouple chemical oxygen demand, ammonia nitrogen, total phosphorus and water toxicity indexes from the spectral signals, and multi-parameter parallel inversion is achieved; and based on the inversion index and the spectral dynamic trend, the dosage is calculated through an intelligent decision-making algorithm, and accurate dosing of the medicament is driven. According to the invention, through fusion of full spectrum scanning and deep learning, high-precision anti-interference water quality perception is realized, potential risks can be identified and preventive intervention can be carried out, and the intelligent level and economical efficiency of dosing control are improved.

The invention relates to the technical field of traditional Chinese medicine ingredient research, in particular to a construction method and application of a fingerprint spectrum based on ganoderma lucidum antioxidant active ingredients. Firstly, a method for ultrasonic-assisted acidolysis of the ganoderma lucidum crude polysaccharide under the guidance of free radical scavenging activity is constructed, the method is efficient and good in operability, and the free radical scavenging activity of a ganoderma lucidum crude polysaccharide depolymer can be well reflected. The method comprises the following steps: carrying out acidolysis and analysis on 52 batches of ganoderma lucidum polysaccharides from different sources by adopting hydrophilic chromatography-electrospray detector-electrospray ionization mass spectrometry, further combining grey correlation analysis and partial least squares regression analysis, screening out 12 active common peaks, and establishing the fingerprint spectrum based on the ganoderma lucidum antioxidant active components. And finally, respectively analyzing the fingerprints of the standard ganoderma lucidum, ganoderma sinense, ganoderma applanatum, ganoderma atrum and the ganoderma lucidum sample based on the ganoderma lucidum antioxidant active components by utilizing a principal component analysis method and a partial least square discriminant analysis method, so as to realize identification of the ganoderma lucidum variety.

The invention belongs to the field of antibiotic detection and biosensors, and particularly relates to a colorimetric array sensor for distinguishing different types of antibiotics based on platinum-based nano-enzyme and a detection method of the colorimetric array sensor. The colorimetric array sensor is composed of platinum nano-enzymes with three different structures, namely platinum nano-particles PtNPs, platinum nano-flowers PtNFs and gold-core platinum-shell nano-particles Au (at) PtNPs. According to the antibiotic detection method based on the platinum nano-enzyme colorimetric array sensing system, the peroxidase-like activity of the antibiotics is regulated and controlled through the non-specific interaction of the three platinum nano-enzymes (PtNPs, PtNFs and Au-coated PtNPs) and the antibiotics, a unique'fingerprint 'map is generated in combination with TMB-H2O2 chromogenic reaction, and efficient distinguishing and concentration detection of various antibiotics are achieved.

The invention relates to the technical field of medicines, and discloses six adeno-associated virus (AAV) injections carrying novel artificial chemical genetics receptor gene sequences, and a combinatorial chemical genetics scheme formed by the injections and a small molecule agonist quetiapine. Compared with the traditional chemical genetics, the medicine quetiapine with smaller clinical side effect is used as the activating agent for the first time, and a novel chemical genetics system with lower medication risk is developed. The system can effectively reduce the excitatory activity of neurons, avoids the medication risk caused by clozapine or other non-market traditional chemical genetics activators, and improves the feasibility of clinical transformation of the chemical genetics system. In the aspect of application of a novel or traditional chemical genetics system, the combined treatment scheme is applied to intervene in Parkinson's disease (PD) for the first time, abnormal activities of STN and related loops of PD patients can be inhibited, and the dyskinesia symptom can be remarkably improved.

The invention relates to the field of botulinum toxin analysis, in particular to an anti-botulinum toxin antibody capillary immunoelectrophoresis fluorescent fingerprint spectrum construction method and application thereof.According to the construction method, capillary immunoelectrophoresis of the fluorescence technology is used for construction, a fluorescent antibody corresponding to a primary antibody is used as a secondary antibody, and the fluorescent antibody is used as a secondary antibody. The technology for detecting the botulinum toxin antibody through fluorescence capillary immunoelectrophoresis is practically applied, and due to the fact that more signals are generated through fluorescence detection, compared with capillary immunoelectrophoresis based on the chemiluminescence technology, the method has better resolution capacity.

The invention relates to the technical field of medicines, and discloses an adeno-associated virus (AAV) injection carrying an artificial chemical genetics receptor gene sequence, and a combinatorial chemical genetics scheme formed by the adeno-associated virus injection and a small molecule agonist quetiapine. Compared with the traditional chemical genetics, the medicine quetiapine with smaller clinical side effect is used as the activating agent for the first time, and a novel chemical genetics system with lower medication risk is developed. The system can effectively reduce the excitatory activity of neurons, avoids the medication risk caused by clozapine or other non-market traditional chemical genetics activators, and improves the feasibility of clinical transformation of the chemical genetics system. In the aspect of application of a novel or traditional chemical genetics system, the combined treatment scheme is applied to intervene in Parkinson's disease (PD) for the first time, abnormal activities of STN and related loops of PD patients can be inhibited, and the dyskinesia symptom can be remarkably improved.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo and a method for establishing a fingerprint spectrum. Comprising the following steps: S1, preparing a to-be-detected solution and a reference substance solution: grinding Bailing tablets, dissolving the Bailing tablets in a solvent, and extracting to prepare the to-be-detected solution; dissolving a paeoniflorin reference substance in a solvent to prepare a reference substance solution; wherein the extraction solvent is a methanol-water mixed solution; s2, respectively obtaining chromatograms of the to-be-detected solution and the reference substance solution under the same detection conditions by adopting ultra-high performance liquid chromatography, and carrying out qualitative analysis and / or quantitative analysis on chemical components in the chromatograms of the to-be-detected solution; wherein the detection conditions are as follows: the wavelength of a detector is 201 + / -2nm; the elution conditions are as follows: acetonitrile is used as a phase A, water is used as a phase B, and elution is performed through a gradient elution procedure. According to the method, the chemical components in the medicine for treating the leucoderma can be accurately determined, so that the establishment of the fingerprint spectrum database of the medicine for treating the leucoderma can be better realized.

The invention discloses a fluorescent array sensing system based on bionic taste sense, a construction method of the fluorescent array sensing system and application of the fluorescent array sensing system in food safety intelligent detection. According to the system, a functionalized ultrathin Ti3C2 MXene nanosheet serves as a bionic tongue carrier, multicolor silver nanoclusters synthesized by oligonucleotide chains are adsorbed on the surface of the functionalized ultrathin Ti3C2 MXene nanosheet to serve as bionic taste bud cells, different DNA chains can form different fluorescence emissions, the characteristic that fluorescence can be designed is achieved, and the preparation process of signal molecules can be greatly simplified. When a plurality of hazardous substances are added into the sensing array at the same time, the Ti3C2 MXene with strong binding capacity can preferentially capture a target object, and the silver nanoclusters with weak binding capacity can release or adsorb, so that fluorescence signals with different colors are changed, and a characteristic fingerprint spectrum is formed. And in combination with a machine learning algorithm, rapid, accurate, qualitative and quantitative intelligent identification of multiple food hazardous substances can be realized at the same time, and a new strategy is provided for simultaneous detection of multiple hazardous substances in food safety.

The invention is suitable for the technical field of pesticide residue detection, and provides a method for distinguishing and detecting pesticide based on copper-platinum bimetallic nano-enzyme. According to the method, a colorimetric sensing array with three sensing channels is constructed by synthesizing a copper-platinum bimetallic nano-enzyme (CuPtNPs) with oxidase-like activity, laccase-like activity and superoxide dismutase-like activity. A fingerprint spectrum is obtained based on specific effects of different pesticides and enzyme-like activities, and qualitative distinguishing and quantitative detection of various pesticides are realized by combining a machine algorithm. According to the method, nine pesticide residues can be measured at a time, six of the nine pesticide residues can be quantitatively analyzed, and the problem that a traditional method only can be used for single detection is solved. A strategy of constructing three channels based on a single nano-enzyme receptor CuPtNPs has the remarkable advantages of simple synthesis process, convenient operation flow and low detection cost, is good in applicability in actual agricultural product samples such as Chinese cabbages and apples, and provides an efficient solution for high-throughput detection of pesticide residues.

The invention provides a birch bark quality comprehensive evaluation method based on combination of a fingerprint spectrum, chemometrics and a quantitative analysis of multiple components by single marker, and relates to the technical field of quality control and evaluation of birch bark. Comprising the following five steps: preparing a birch bark sample, setting chromatography and mass spectrometry conditions, generating a fingerprint spectrum and evaluating similarity, analyzing chemometrics data, and carrying out quantitative analysis by using a single-marker multi-evaluation method. Multiple components can be quantified at the same time, and the quality of the birch bark is comprehensively evaluated. The multi-component analysis mode can cover the key effective components in the medicinal materials, so that the quality evaluation deviation caused by neglecting other components is avoided, and the reference can be provided for the quality control and evaluation of the birch bark accurately, simply and conveniently in combination with a chemometrics method through the established birch bark fingerprint spectrum and the multi-index component determination method.

The invention provides a fingerprint spectrum and multi-component content determination method of a calamus-far nerve calming mixture, and relates to the field of quality control of traditional Chinese medicines. 31 common peaks are calibrated by establishing a fingerprint spectrum, and 24 common peaks can clearly belong to polygala tenuifolia. Meanwhile, synchronous content determination of seven components including chlorogenic acid, caffeic acid, ferulic acid, liquiritin, 2, 3, 5, 4 '-tetrahydroxy stilbene-2-O-beta-D-glucoside, 3, 6'-dimustard acyl sucrose and rosmarinic acid is realized. The method is good in precision, high in repeatability and stable and reliable in result, the medicine quality can be comprehensively and accurately reflected and controlled, and powerful support is provided for guaranteeing the safety and effectiveness of the calamus-far Ningmind mixture.

This invention relates to the field of quality testing technology for traditional Chinese medicine preparations, specifically providing a method for constructing a fingerprint spectrum and a quality testing method for a Qingfei Decoction compound preparation. In the fingerprint spectrum construction method, the chromatographic conditions include: using octadecylsilane-bonded silica gel as the packing material, acetonitrile as mobile phase A, and an aqueous solution containing phosphoric acid as mobile phase B, obtaining 10 common characteristic peaks under a specific elution program. Under the conditions of this invention, the characteristic peaks are well separated, each peak has a good peak shape, and the method can comprehensively, clearly, and effectively detect the quality of the Qingfei Decoction compound preparation.

The present invention aims at the current situation that the existing content determination and fingerprint spectrum lack the analysis of the existence state of the index components in quality evaluation, and based on the fact that the component state is directly related to the stability and effectiveness of the preparation, provides a method for evaluating the quality of Danshen injection based on interface diffusion. The present invention innovatively reflects the difference in the existence state of Danshen medicinal components according to the characteristics of interface distribution, creates a method for evaluating the quality difference of Danshen injection from the interface diffusion at the molecular state level, solves the technical bottleneck that the traditional quality evaluation is difficult to explain the difference in the stability and efficacy of Danshen injection, and helps to improve the standardization and orderliness of the production process of Danshen injection.

The invention discloses a tulip EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker as well as a primer composition and application thereof. According to the invention, 15 pairs of tulip EST-SSR molecular markers and a primer composition thereof are obtained through screening, a to-be-detected tulip variety resource DNA is extracted, the 15 pairs of EST-SSR primers or synthetic fluorescent primers are respectively used for PCR amplification, a PCR product is subjected to PAGE detection or fluorescent capillary electrophoresis, and the EST-SSR fingerprint of the tulip variety resource is constructed. The method and the basis are provided for tulip variety breeding, variety authenticity identification, known variety library construction and the like.

The present invention discloses a multi-component quality control method for Tianfoshen oral liquid, which comprises (1) preparing a test solution: (2) preparing a mixed reference solution; determining the HPLC fingerprint of the Tianfoshen oral liquid and calibrating the effective components. The present invention screens out the best mobile phase composition, detection wavelength, chromatographic column, column temperature and other analytical conditions through a large number of experiments. It is shown through multiple experimental verifications that the detection method provided by the present invention can simultaneously detect multiple active components such as ginsenosides and asparagus saponins with similar polarity, so that the quality of the Tianfoshen oral liquid can be comprehensively, objectively and accurately detected and evaluated, which is of great significance for ensuring clinical efficacy.

This invention provides a method for constructing a fingerprint spectrum of the Astragalus-Hirudo-Tongluo formula and a method for quality evaluation of its capsules, belonging to the field of traditional Chinese medicine analysis technology. This invention sequentially detects samples using an ultraviolet detector and an evaporative light scattering detector. Under the same chromatographic conditions, using two different detectors for the same sample allows for the simultaneous acquisition of fingerprint spectra of compounds with both strong and weak ultraviolet absorption. This method maximizes the detection of chemical components in the Astragalus-Hirudo-Tongluo formula while saving time and effort. The method is simple, easy to implement, highly sensitive, specific, and robust. Based on the fingerprint spectrum method, this invention identifies chromatographic peaks, thereby achieving better quality control of the Astragalus-Hirudo-Tongluo formula.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and relates to a method for simultaneously determining fingerprints and multiple components of a rhizoma atractylodis macrocephalae medicinal material and an extract of the rhizoma atractylodis macrocephalae medicinal material. The invention provides the detection method of the fingerprint spectrum of the rhizoma atractylodis macrocephalae medicinal material and the extract thereof and the construction method of the standard fingerprint spectrum, which are of great significance in enhancing qualitative and quantitative control of the rhizoma atractylodis macrocephalae and ensuring that the medicinal effect of the rhizoma atractylodis macrocephalae medicinal material is maintained in the process from production, transportation to clinical application; on the basis of the existing fingerprint spectrum research, the common peak distribution characteristics of the fingerprint spectrum of the rhizoma atractylodis macrocephalae medicinal material and the volatile oil are further determined, and an HPLC simultaneous determination method for the five components of atractylone, atractylenolides I-III and beta-caryophyllene is established by a wavelength gradient method, so that a solid foundation is laid for drawing up the quality standard of the rhizoma atractylodis macrocephalae medicinal material, the rhizoma atractylodis macrocephalae volatile oil and the preparation of the rhizoma atractylodis macrocephalae medicinal material and the rhizoma atractylodis macrocephalae volatile oil.

The invention discloses a conjugated oligomer as well as a preparation method and application thereof. The structural characteristics of the conjugated oligomer are shown as a formula I, a formula II or a formula III. The compound molecule is composed of a rigid conjugated skeleton and a flexible side chain, and can be used as a fluorescent sensing element to realize bacterial species recognition. According to the method, fingerprint spectrums of different bacterial standard samples are established, pattern recognition is performed on bacterial samples of unknown types, and recognition results are compared with the fingerprint spectrums established in the earlier stage, so that rapid identification of bacterial types is realized. The series of compounds also show certain antibacterial activity, the antibacterial ability of the compounds is related to molecular structure parameters (such as conjugated skeleton type, conjugated skeleton length and terminal cation group type), and the antibacterial ability of the compounds can be optimized by systematically regulating and controlling the structural parameters. # imgabs0 #

The present invention discloses a method for constructing a fingerprint of Gualou Niu Bang decoction, relating to the technical field of analytical chemistry. The method is simple and has high precision, stability, and repeatability. Determination revealed that seven components, including geniposide, liquiritin, hesperidin, arctiin, baicalin, melaleucalyptol A-7-O-β-D-glucuronide, and wogonin, can be used as common characteristic peaks in the fingerprint of Gualou Niu Bang decoction for identification research.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The invention belongs to the technical field of biology, and particularly relates to a sophora tonkinensis SSR molecular marker primer group, a kit as well as application and a method of the sophora tonkinensis SSR molecular marker primer group. The sophora tonkinensis SSR molecular marker primer group comprises 15 pairs of primers, the nucleotide sequences of the 15 pairs of primers are sequentially shown as SEQ ID NO.1-SEQ ID NO.30, and the upstream primer in each primer pair is marked with a fluorophore. The sophora tonkinensis SSR molecular marker primer provided by the invention is high in polymorphism, and can effectively reveal the genetic diversity of sophora tonkinensis; a fingerprint database of sophora tonkinensis germplasm is also constructed, and the variety of sophora tonkinensis to be detected can be identified through comparison; the number of the SSR molecular markers of the sophora tonkinensis is increased, a tool is provided for genetic diversity research and germplasm resource analysis of the sophora tonkinensis, and a theoretical basis is provided for protection and utilization of sophora tonkinensis resources.

The invention provides a primer group for identifying astragalus mongholicus and an identification method of astragalus mongholicus, and belongs to the technical field of molecular markers, the primer group comprises a primer 186781F and a primer 186781R; the nucleotide sequence of the 186781F is as shown in SEQ ID NO. 1, and the nucleotide sequence of the 186781R is as shown in SEQ ID NO. 2. The primer group provided by the invention is designed aiming at the SSR molecular marker, and the SSR molecular marker has the advantages of uniform distribution, rich polymorphism, good amplification repeatability, clear banding pattern and the like in an astragalus membranaceus genome, and is suitable for detection of a common denaturing polyacrylamide gel electrophoresis detection platform and a capillary fluorescence detection platform; the method can be further applied to the research fields of astragalus genetic diversity analysis, variety identification, DNA fingerprint construction, molecular marker-assisted breeding and the like.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and discloses a quality detection method of rhizoma atractylodis macrocephalae fried with fructus aurantii, which is characterized by comprising the following steps: performing qualitative analysis on rhizoma atractylodis macrocephalae fried with fructus aurantii by respectively adopting a thin-layer chromatography and an HPLC (High Performance Liquid Chromatography) characteristic chromatogram; a high performance liquid phase external standard curve method is adopted to carry out quantitative analysis on atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin in the fructus aurantii fried bighead atractylodes rhizome. The invention establishes a thin-layer identification method for rhizoma atractylodis macrocephalae and fructus aurantii in a fructus aurantii fried rhizoma atractylodis macrocephalae processed product; according to the method for establishing the fingerprint spectrum, the similarity of 10 batches of rhizoma atractylodis macrocephalae fried with fructus aurantii ranges from 0.991 to 1.000; the method for determining the content of atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin is established, scientific support is provided for formulating the quality standard of the characteristic processing decoction pieces of the temporary prescription, and effective guarantee is provided for clinical application of the decoction pieces.

The present invention provides a novel integrated platform that enables the verification of manufacturing processes for complex natural products, as well as the standardization and evaluation of complex natural products. This platform allows for the "system-wide" characterization of complex mixtures contained in therapeutic and / or health-promoting products based on or composed of natural substances, without limiting the approach to reductionist chemical approaches targeting single markers. This is achieved through the integration of qualitative and quantitative fingerprint characterization data metabolomics and physical and biophysical activity data for the mixtures, thereby providing a product characterization that forms the basis for verifying manufacturing processes that can ensure the reproducibility of efficacy and safety across different batches of the product. The analytical platform described herein enables the assurance of a complex natural product as a whole, by considering all the characteristics of its quality and efficacy, through a multidimensional analytical approach, in order to ensure reproducibility regarding efficacy and safety.

The present invention relates to a method for constructing a Xiebai medicinal material fingerprint and determining the content of Xiebai index components, including: taking Xiebai medicinal material to prepare a test solution; taking Macrostemonoside I, 25S-Timosaponin B II and 25R-Timosaponin B II to prepare a reference solution; injecting the test solution and the reference solution into a liquid chromatography-mass spectrometer respectively, and obtaining the fingerprint of the Xiebai medicinal material according to the liquid chromatography-mass spectrometry method, and performing content determination on the above-mentioned index components. By the method for constructing Xiebai medicinal material fingerprint and content determination provided by the present invention, the evaluation standard of Xiebai is fully improved, and the effectiveness of clinical use of Xiebai medicinal material is guaranteed.

This invention relates to the field of traditional Chinese medicine analysis, and particularly to a method for determining the content of multiple components in Terminalia chebula and constructing its characteristic fingerprint spectrum. The method provided by this invention can simultaneously determine chebulin, gallic acid, 4-galloshikimic acid, 5-galloshikimic acid, punicalin (punicalin A&B), corilagin, 1,3,6-tri-O-galloyl-β-D-glucose, chebulin, urolithin M5, chebulin tannic acid, chebulin-lintannic acid, and ellagic acid in Terminalia chebula. It exhibits high sensitivity, good precision, repeatability, and stability, and can be used to detect the tannin content in the extract at each step of the tannin extraction process, as well as for related detection in the study and quality evaluation of tannin components in Terminalia chebula. It can also be used to construct the characteristic fingerprint spectrum of Terminalia chebula. This method can also be used for determining the content of tannin compounds and constructing their characteristic fingerprint spectrum in Terminalia chebula, which contains similar tannin compounds to Terminalia chebula.