The invention discloses a method for establishing a control
fingerprint spectrum of Shengbai Koufuye, which comprises the following steps that 1, a
test solution is prepared, namely, multiple batchesof qualified Shengbai Koufuye are put into a
volumetric flask and the volume is fixed; 2, a reference substance solution is prepared, a reference substance
icariin is taken, and the volume is fixed; 3, chromatographic conditions are that octadecylsilane chemically bonded silica is taken as a filler,
acetonitrile is taken as a mobile phase A,
formic acid water is taken as a mobile phase B, gradientelution of the mobile phase is carried out, the detection
wavelength ranges from 200 nm to 300 nm, the
column temperature is 25-35 DEG C, the flow velocity is 0.9 ml / min to 1.1 ml / min, and the numberof theoretical plates is not less than 10000 calculated according to
icariin peaks; 4, in the determination method, the reference substance solution and the
test solution are respectively absorbed, and the solutions are injected into an ultra-high performance liquid chromatograph for determination to obtain a chromatogram; and step 5, common peak confirmation is conducted: the chromatograms of the multiple batches of qualified drugs are processed through a computer model to form a unified chromatogram, and the chromatographic peak with the
peak area accounting for more than or equal to 0.8% of the total
peak area, good peak pattern and high separation degree is selected as a common peak.