57 results about "Chemogenetics" patented technology

The invention belongs to the field of wastewater treatment and industrial automatic control, and particularly relates to an intelligent dosing control method based on water quality online monitoring. The method comprises the following steps: capturing a water body spectrum fingerprint spectrum by using a full-spectrum scanning module to obtain a characteristic signal sequence; a chemical informatics molecular algorithm is introduced for preprocessing and feature enhancement, and molecular fingerprint information is recognized; a convolutional neural network model is constructed to decouple chemical oxygen demand, ammonia nitrogen, total phosphorus and water toxicity indexes from the spectral signals, and multi-parameter parallel inversion is achieved; and based on the inversion index and the spectral dynamic trend, the dosage is calculated through an intelligent decision-making algorithm, and accurate dosing of the medicament is driven. According to the invention, through fusion of full spectrum scanning and deep learning, high-precision anti-interference water quality perception is realized, potential risks can be identified and preventive intervention can be carried out, and the intelligent level and economical efficiency of dosing control are improved.

The invention relates to the technical field of medicines, and discloses an adeno-associated virus (AAV) injection carrying an artificial chemical genetics receptor gene sequence, and a combinatorial chemical genetics scheme formed by the adeno-associated virus injection and a small molecule agonist quetiapine. Compared with the traditional chemical genetics, the medicine quetiapine with smaller clinical side effect is used as the activating agent for the first time, and a novel chemical genetics system with lower medication risk is developed. The system can effectively reduce the excitatory activity of neurons, avoids the medication risk caused by clozapine or other non-market traditional chemical genetics activators, and improves the feasibility of clinical transformation of the chemical genetics system. In the aspect of application of a novel or traditional chemical genetics system, the combined treatment scheme is applied to intervene in Parkinson's disease (PD) for the first time, abnormal activities of STN and related loops of PD patients can be inhibited, and the dyskinesia symptom can be remarkably improved.

The invention discloses a fluorescent array sensing system based on bionic taste sense, a construction method of the fluorescent array sensing system and application of the fluorescent array sensing system in food safety intelligent detection. According to the system, a functionalized ultrathin Ti3C2 MXene nanosheet serves as a bionic tongue carrier, multicolor silver nanoclusters synthesized by oligonucleotide chains are adsorbed on the surface of the functionalized ultrathin Ti3C2 MXene nanosheet to serve as bionic taste bud cells, different DNA chains can form different fluorescence emissions, the characteristic that fluorescence can be designed is achieved, and the preparation process of signal molecules can be greatly simplified. When a plurality of hazardous substances are added into the sensing array at the same time, the Ti3C2 MXene with strong binding capacity can preferentially capture a target object, and the silver nanoclusters with weak binding capacity can release or adsorb, so that fluorescence signals with different colors are changed, and a characteristic fingerprint spectrum is formed. And in combination with a machine learning algorithm, rapid, accurate, qualitative and quantitative intelligent identification of multiple food hazardous substances can be realized at the same time, and a new strategy is provided for simultaneous detection of multiple hazardous substances in food safety.

The invention provides a fingerprint spectrum and multi-component content determination method of a calamus-far nerve calming mixture, and relates to the field of quality control of traditional Chinese medicines. 31 common peaks are calibrated by establishing a fingerprint spectrum, and 24 common peaks can clearly belong to polygala tenuifolia. Meanwhile, synchronous content determination of seven components including chlorogenic acid, caffeic acid, ferulic acid, liquiritin, 2, 3, 5, 4 '-tetrahydroxy stilbene-2-O-beta-D-glucoside, 3, 6'-dimustard acyl sucrose and rosmarinic acid is realized. The method is good in precision, high in repeatability and stable and reliable in result, the medicine quality can be comprehensively and accurately reflected and controlled, and powerful support is provided for guaranteeing the safety and effectiveness of the calamus-far Ningmind mixture.

The invention discloses a tulip EST-SSR (expressed sequence tag-simple sequence repeat) molecular marker as well as a primer composition and application thereof. According to the invention, 15 pairs of tulip EST-SSR molecular markers and a primer composition thereof are obtained through screening, a to-be-detected tulip variety resource DNA is extracted, the 15 pairs of EST-SSR primers or synthetic fluorescent primers are respectively used for PCR amplification, a PCR product is subjected to PAGE detection or fluorescent capillary electrophoresis, and the EST-SSR fingerprint of the tulip variety resource is constructed. The method and the basis are provided for tulip variety breeding, variety authenticity identification, known variety library construction and the like.

This invention provides a method for constructing a fingerprint spectrum of the Astragalus-Hirudo-Tongluo formula and a method for quality evaluation of its capsules, belonging to the field of traditional Chinese medicine analysis technology. This invention sequentially detects samples using an ultraviolet detector and an evaporative light scattering detector. Under the same chromatographic conditions, using two different detectors for the same sample allows for the simultaneous acquisition of fingerprint spectra of compounds with both strong and weak ultraviolet absorption. This method maximizes the detection of chemical components in the Astragalus-Hirudo-Tongluo formula while saving time and effort. The method is simple, easy to implement, highly sensitive, specific, and robust. Based on the fingerprint spectrum method, this invention identifies chromatographic peaks, thereby achieving better quality control of the Astragalus-Hirudo-Tongluo formula.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The invention belongs to the technical field of biology, and particularly relates to a sophora tonkinensis SSR molecular marker primer group, a kit as well as application and a method of the sophora tonkinensis SSR molecular marker primer group. The sophora tonkinensis SSR molecular marker primer group comprises 15 pairs of primers, the nucleotide sequences of the 15 pairs of primers are sequentially shown as SEQ ID NO.1-SEQ ID NO.30, and the upstream primer in each primer pair is marked with a fluorophore. The sophora tonkinensis SSR molecular marker primer provided by the invention is high in polymorphism, and can effectively reveal the genetic diversity of sophora tonkinensis; a fingerprint database of sophora tonkinensis germplasm is also constructed, and the variety of sophora tonkinensis to be detected can be identified through comparison; the number of the SSR molecular markers of the sophora tonkinensis is increased, a tool is provided for genetic diversity research and germplasm resource analysis of the sophora tonkinensis, and a theoretical basis is provided for protection and utilization of sophora tonkinensis resources.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The present invention provides a novel integrated platform that enables the verification of manufacturing processes for complex natural products, as well as the standardization and evaluation of complex natural products. This platform allows for the "system-wide" characterization of complex mixtures contained in therapeutic and / or health-promoting products based on or composed of natural substances, without limiting the approach to reductionist chemical approaches targeting single markers. This is achieved through the integration of qualitative and quantitative fingerprint characterization data metabolomics and physical and biophysical activity data for the mixtures, thereby providing a product characterization that forms the basis for verifying manufacturing processes that can ensure the reproducibility of efficacy and safety across different batches of the product. The analytical platform described herein enables the assurance of a complex natural product as a whole, by considering all the characteristics of its quality and efficacy, through a multidimensional analytical approach, in order to ensure reproducibility regarding efficacy and safety.

This invention relates to the field of traditional Chinese medicine analysis, and particularly to a method for determining the content of multiple components in Terminalia chebula and constructing its characteristic fingerprint spectrum. The method provided by this invention can simultaneously determine chebulin, gallic acid, 4-galloshikimic acid, 5-galloshikimic acid, punicalin (punicalin A&B), corilagin, 1,3,6-tri-O-galloyl-β-D-glucose, chebulin, urolithin M5, chebulin tannic acid, chebulin-lintannic acid, and ellagic acid in Terminalia chebula. It exhibits high sensitivity, good precision, repeatability, and stability, and can be used to detect the tannin content in the extract at each step of the tannin extraction process, as well as for related detection in the study and quality evaluation of tannin components in Terminalia chebula. It can also be used to construct the characteristic fingerprint spectrum of Terminalia chebula. This method can also be used for determining the content of tannin compounds and constructing their characteristic fingerprint spectrum in Terminalia chebula, which contains similar tannin compounds to Terminalia chebula.

This invention provides a quality testing method for nasal drops used to treat allergic rhinitis, belonging to the field of pharmaceutical testing. Specifically, it includes the following steps: S1. Preparing a test solution; S2. Preparing a mixed reference solution; S3. Separately sampling the reference solution and the test solution for analysis; S4. Generating a reference fingerprint chromatogram. This invention can present all different types of components of the medicinal materials on a single chromatogram through a single test, allowing for simultaneous determination of multiple components and achieving comprehensive and accurate quality control of Artemisia annua and Scutellaria baicalensis nasal drops. The quality assessment method provided by this invention has optimized chromatographic conditions and undergone methodological validation, possessing the advantages of method stability, good repeatability, and stable and reliable test results.

The application discloses a method for screening a quality marker of Radix Paeoniae Rubra based on an anticoagulation spectrum-effect relationship, mainly comprising the following steps: preparing control samples and Radix Paeoniae Rubra sample materials, determining a preparation method of the Radix Paeoniae Rubra sample and optimal chromatographic conditions for separating the Radix Paeoniae Rubra sample, and constructing a fingerprint of the Radix Paeoniae Rubra sample; using a blood coagulation instrument to determine an in-vitro anticoagulation activity of Radix Paeoniae Rubra extracts; analyzing experimental data to determine the quality marker of Radix Paeoniae Rubra based on the anticoagulation spectrum-effect relationship. The efficacy and common peaks of the fingerprint are analyzed by a grey correlation degree analysis method, and the quality marker of Radix Paeoniae Rubra for anticoagulation is screened. The method overcomes the fact that the prior art does not have a related technology for determining the quality marker of Radix Paeoniae Rubra for anticoagulation by the grey correlation degree analysis method.

The application provides a HPLC-based method for constructing a fingerprint spectrum of Tianyang Qianggu Yin and application thereof, and belongs to the technical field of traditional Chinese medicine detection.The method provided by the application comprises the steps of preparing a test sample solution, preparing a mixed control sample solution, HPLC determination, fingerprint spectrum construction and content determination.The application first establishes a HPLC fingerprint spectrum suitable for water-soluble extracts of Tianyang Qianggu Yin compound, and simultaneously realizes accurate content determination of six components, i.e., neotamanoxin, naringin, alpinumisine A, alpinumisine B, alpinumisine C and icariin.The application has strong specificity, high precision, good repeatability and excellent accuracy.The application provides scientific and controllable technical support for the production quality consistency and efficacy stability of Tianyang Qianggu Yin, and can be used for quality control and batch consistency evaluation of the compound preparation.

The application discloses a kind of material evaluation methods, pharmaceutical compositions and application of sophora fruit products.It is specific, disclose a kind of material evaluation methods of sophora fruit products, it includes the following steps: S1, using liquid chromatography, the fingerprint of the sophora fruit product of different processing time is established, and common characteristic peak is extracted;S2, the hemostatic effect of sophora fruit product of different processing time is determined;S3, according to the characteristic peak data of the sophora fruit product of different processing time and its hemostatic effect data, statistical analysis is carried out by chemometrics method, and the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect of the hemostatic effect

The invention discloses a red yeast rice fingerprint spectrum detection method. The fingerprint detection method is developed for the monacolin compound in the monascus, whether the to-be-detected sample contains the monascus meeting the quality requirement or not can be directly judged by comparing the similarity of the to-be-detected sample and the contrast fingerprint, the detection efficiency is improved, and the detection cost is reduced.

This invention provides a method for screening the active ingredient groups of Xinsu Ning capsules, relating to the technical field of quality control and pharmacodynamic substance analysis of traditional Chinese medicine. The screening method for the active ingredient groups of Xinsu Ning capsules provided by this invention integrates non-targeted chemical component analysis, in vivo prototype and metabolite detection, network pharmacology correlation analysis, and fingerprint spectrum construction, enabling a systematic evaluation of the in vivo exposure characteristics, target regulatory potential, and quality consistency of candidate components. This method can identify chemical components related to pharmacodynamic effects from complex systems, providing an operable technical path for the quality control and pharmacodynamic substance basis research of Xinsu Ning capsules.

The application belongs to the technical field of medicine detection, and particularly relates to a kind of fingerprint spectrum of qi-regulating and blood-activating preparation and a method for establishing, the qi-regulating and blood-activating preparation is made of Litsea rotundifolia, moxa tablet, chuanxiong, and baiyunqing, the fingerprint spectrum is determined by UPLC-MS method, compared with ordinary liquid chromatography, has the advantages of higher resolution, more sensitive detection, shorter separation time, and more explicit chemical components; at the same time, it is helpful to establish a perfect quality control system of product, and is helpful to guarantee the quality of medicine and clinical efficacy.

The application provides a method for establishing an HPLC fingerprint of Juanbi decoction, and belongs to the technical field of chemical analysis. The method comprises the following steps: S1. preparing a material reference of Juanbi decoction; S2. preparing a single-ingredient medicinal slice sample; S3. preparing a negative sample; S4. preparing a standard solution; and S5. performing chromatographic analysis. The method can help screen out a pharmacodynamic material basis of JBD for resisting RA, and the research provides a solid research basis for establishing a quality evaluation standard for component screening of JBD for resisting RA and development of JBD products.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a fingerprint spectrum analysis method of seven-ingredient radix stephaniae tetrandrae and radix astragali granules. The fingerprint spectrum analysis method of the seven-ingredient radix stephaniae tetrandrae and radix astragali granules comprises the following steps: preparing a test solution; preparing a reference substance solution; determining the fingerprint spectrum of the seven-ingredient radix stephaniae tetrandrae and radix astragali granules or the seven-ingredient radix stephaniae tetrandrae and radix astragali water extract by high performance liquid The detection method is high in universality and low in detection cost, and the quantitative fingerprint spectrum of the product quality can be comprehensively reflected.

The application discloses a kind of based on HPLC Jian'erle granule multi-component fingerprint construction and active evaluation method, belong to the technical field of drug analysis.The application is based on ultra-high performance liquid chromatography technology, by optimizing drug extraction and chromatographic detection method, provide the fingerprint construction method of analysis Jian'erle granule drug quality;The method comprises the steps of ultrasonic dissolution Jian'erle granule, with 0.1% phosphoric acid and acetonitrile as mobile phase for liquid chromatography detection.According to the construction method provided by the application, a fingerprint consisting of 12 characteristic peaks is obtained, of which 10 characteristic peaks are identified to compounds.The results of the methodological experiment show that the construction method provided by the application has a similarity of >0.999 between 10 batches of Jian'erle granules, and has outstanding specificity, precision, repeatability and stability.The application provides a new strategy for efficient quality detection and quality control of Jian'erle granules, and has a broad application prospect.

The present application relates to a kind of fingerprints of radix tetrapanacis and its application in medicinal material quality control, the radix tetrapanacis HPLC fingerprint spectrum established in the present application overcomes the shortcomings of single detection index in prior art, can not reflect the internal quality of medicinal materials, the provided radix tetrapanacis HPLC fingerprint spectrum establishment method is high in precision, good stability and repeatability, by comparing the presence or absence of common peaks in the obtained fingerprint spectrum, the quality of radix tetrapanacis can be effectively characterized, and it has important significance for ensuring clinical efficacy.

The application belongs to the technical field of medicine detection, and particularly relates to a detection method of Fuyanxiao preparation, which is made of seven medicinal materials of Oxalis corniculata L., Patrinia, Radix Trichosanthis, Radix et Rhizoma Rhei, Cortex Moutan, Atractylodes and Liriodendron, and the detection method is to use HPLC method, acetonitrile as mobile phase A, 0.2% phosphoric acid aqueous solution as mobile phase B for gradient elution to determine the fingerprint and / or characteristic spectrum. The establishment of the detection method is beneficial to comprehensively monitor the product quality, and further helps to guarantee the medicine quality and clinical efficacy.

The application discloses a characteristic spectrum construction of shengqi oral liquid, and comprises the following steps: S100, preparation of control sample solution; S200, preparation of test sample solution; S300, high performance liquid chromatography analysis; wherein, the high performance liquid chromatography analysis comprises detecting each control sample solution and the test sample solution by using an evaporative light scattering detector and an ultraviolet PDA detector respectively, and obtaining corresponding fingerprint spectrum and standard characteristic spectrum respectively. The application further discloses a dynamic monitoring method for the quality of shengqi oral liquid. The method can comprehensively monitor various animal and plant components of the shengqi oral liquid, and is simple and fast in operation, realizes real-time monitoring of multiple quality indexes of the shengqi oral liquid, can comprehensively control and evaluate the internal quality of the shengqi oral liquid, and guarantees the stability, effectiveness and safety of the drug quality.

The invention belongs to the technical field of molecular biology, germplasm resource science and molecular breeding, and discloses an artemia 5K liquid phase chip based on a targeted capture sequence, a preparation method and application thereof, and the liquid phase chip comprises 4329 SNP sites. Compared with a previous fingerprint spectrum developed based on SNP molecular markers, the artemia liquid-phase chip disclosed by the invention contains more SNP markers, the identification accuracy is higher, the later detection cost is lower, the design is flexible, and interested marker sites can be added at any time in the later period.

The invention discloses a fingerprint spectrum detection method of a consistent decoction reference sample and application, and relates to the technical field of traditional Chinese medicine detection. According to the fingerprint detection method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 28-32 DEG C; the detection wavelength is 0 to 25 minutes, and the wavelength is 278 to 282nm; in 25-66 minutes, the wavelength is 318-322 nm; the time is 66 to 80 minutes, and the wavelength is 278 to 282nm; the time is 80 to 90 minutes, and the wavelength is 318 to 322 nm; the mobile phase A is acetonitrile, and the mobile phase B is a 0.09-0.11 wt% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the method, digitonin C, 4-cumaric acid, ferulic acid, rutin, verbascoside, senkyunolide I and ligustilide in the consistent decoction can be detected at a time, and the method is high in accuracy, good in repeatability, high in durability and good in specificity.

The application provides a pattern recognition method for comprehensively evaluating the quality of Chinese medicinal Exocarpium Citri Grandis based on color, aroma and non-volatile organic matter, and belongs to the technical field of drug quality control. The method comprises the following steps: collecting Chinese medicinal Exocarpium Citri Grandis samples with accurate source information, evaluating the color and aroma and collecting non-volatile organic matter data through crushing and brewing, establishing the color, aroma and non-volatile component fingerprint of the Exocarpium Citri Grandis sample, and obtaining the overall chemical information of the sample; then, the overall chemical information of the Exocarpium Citri Grandis sample is subjected to normalized data processing, combined with chemometrics, multivariate cluster analysis and factor analysis, and the characteristic sample data for establishing a discriminant function are screened out; a stepwise discriminant analysis method is used to extract characteristic variables from the screened sample data, a discriminant function is established, and meanwhile, the characteristic variables are used in combination with machine learning means to perform prediction analysis on unknown Exocarpium Citri Grandis samples, so that the pattern recognition of the quality of the Exocarpium Citri Grandis sample is completed.

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a construction method and application of a sophora japonica L. fingerprint. The construction method comprises preparation of a test sample solution, gradient elution with acetonitrile as mobile phase A and phosphoric acid aqueous solution as mobile phase B, and adoption of a specific gradient elution procedure. The construction method can obtain 11 common characteristic peaks, the separation degree between the common characteristic peaks is good, the characteristic peaks are uniformly distributed, and the effective components of the common characteristic peaks can be identified. The effective chemical components can be used to accurately identify sophora japonica L., overcome the defects that the quality detection and identification of sophora japonica L. cannot be comprehensively, clearly and effectively performed due to the interference caused by the complex chemical components in the sophora japonica L. medicinal materials, and the method is suitable for detection of sophora japonica L. products. Further, the construction method has the advantages of short detection time, high precision, good repeatability and stability, and can comprehensively monitor the quality of sophora japonica L.

This invention relates to the field of plant molecular marker technology, specifically to an EST-SSR molecular marker primer set developed based on the transcriptome sequence of *Prunus campanulata* and its applications. The EST-SSR molecular marker primer set contains 19 pairs of primers, with sequences shown in SEQ ID Nos. 1–38. The EST-SSR molecular marker primers developed based on *Prunus campanulata* transcriptome data exhibit greater accuracy, stability, and effectiveness compared to traditional molecular markers. Furthermore, the EST-SSR markers are mostly distributed in regions related to gene coding regions or regulatory sequences, providing a theoretical basis and important technical means for *Prunus campanulata* germplasm resource identification, genetic diversity analysis, molecular-assisted breeding, and fingerprinting, and have promising application prospects.

The invention relates to a gracilaria cpSSR (simple sequence repeat) marker primer combination and application thereof. The SSR molecular marker primer combination comprises the following five pairs of primer combinations: CpSSR-6-451, CpSSR-11-97, CpSSR-11-485, CpSSR-10-162 and CpSSR-12-86. The gracilaria cpSSR marker primer combination provided by the invention has the beneficial effects that a group of SSR loci with high polymorphism and high resolution and specific primers thereof are screened from chloroplast genomes, so that the resolving power and stability of the existing molecular marker are remarkably improved; the marker combination can provide objective and accurate identification basis for gracilaria species from genetic essence, effectively distinguish species with similar forms, solve the problem of difficult identification of traditional methods, can be used for gracilaria DNA fingerprint spectrum construction, germplasm resource identification, genetic relationship analysis and population genetic diversity research, and has wide application prospects. And a reliable technical means is provided for protection, development and improved variety breeding of gracilaria germplasm resources.