Identification method of paris polyphylla
A technology of Aescinus aesculus and identification method, which is applied in the field of identification of Aescinus aescini, which can solve the problems of skyrocketing market prices, flooding of commodity markets with rhizomes, and constraints on sustainable development.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0054] Embodiment 1 infrared fingerprint identification
[0055] Detection method:
[0056] Sample processing: Take the original medicinal material and grind it, pass it through a No. 5 sieve for later use. Take each sample powder and dry at 60°C for 6h. Take 4 mg of each sample separately (to make the light transmittance of the strongest absorption peak below 10), add 200 mg of KBr, mix and grind evenly under an infrared lamp, put it into a mold, press into tablets, and the spectral resolution is 4 cm -1 , measurement area 4000-400cm -1 , medium-speed scanning, scanning times 16 times, instant removal of moisture and CO during scanning 2 The background interference is to ensure the comparability of the maps. In order to reduce the error caused by sample loading, each sample was ground evenly and scanned in parallel 3 times, that is, after each measurement, the sample was poured out and reloaded into a tablet to ensure that the spectrum collection was representative, and t...
Embodiment 2 7
[0070] The composite identification method of embodiment 2 Aescin
[0071] Although no cases of inconsistency with the identification of infrared fingerprints have been found in the current experiments, in order to avoid omissions in the identification of medicinal materials as much as possible and ensure higher accuracy of identification, the present invention also uses a composite identification method, that is, infrared, X-ray diffraction, etc. methods used in combination.
[0072] Wherein, the infrared identification method is as in Example 1.
[0073] The X-ray powder diffraction method is as follows:
[0074] Detection method:
[0075] Sample processing: take a sample of the rhizome of Aesculus chinensis, crush it, dry it at 60°C for 6 hours, and pass it through a 100-mesh sieve for later use. Test conditions: CuKα radiation; scan mode: qualitative, step scan; voltage / current: 36kV / 20mA; scan speed: 8° / min; DS=SS=1°, RS=0.3mm; step width: 0.02°; scan Range 2θ: 5-65°....
Embodiment 3 7
[0088] The composite identification method of embodiment 3 Aescin
[0089] In addition to the method of combining infrared and X-ray powder diffraction identification in Example 2, UPLC can also be added in the present invention.
[0090] Infrared and X-ray powder diffraction identification methods are as in Example 2.
[0091] UPLC identification method is as follows:
[0092] Chromatographic conditions
[0093] ACQUITY HSS C18 column (2.1mm×75mm, 1.7μm); ELSD detector drift tube temperature 55°C, nitrogen pressure 40psi, gain 500, cooling mode; column temperature 40°C; flow rate 0.2mL min -1 ; Injection volume: 2 μL; The mobile phase is acetonitrile (A)-water (B), and the gradient elution program is shown in Table 4. Mixed reference substance chart see Figure 5 .
[0094] Table 4 Gradient elution program
[0095]
[0096] Preparation of the test solution
[0097] Grind the dried rhizome of Aescin, pass through a 40-mesh sieve, accurately weigh 0.500 g of Rhizome...
PUM
Property | Measurement | Unit |
---|---|---|
transmittivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com