6 results about "Aesculus chinensis" patented technology

This invention discloses a method for preparing and applying aescin in cosmetics, belonging to the field of plant active ingredient extraction technology. The method for preparing aescin includes the following steps: adding horse chestnut seeds to an organic solvent, then adding an alkaloid, heating and refluxing to extract, and then removing the organic solvent to obtain a crude extract; dissolving the crude extract in water, adding a cyclic oligosaccharide, stirring and reacting, then adding an organic acid, heating and reacting, and finally purifying to obtain aescin. The preparation method of this invention can improve both the extraction rate and retention rate of aescin.

The application discloses a method for preparing high-value cellobiose from a medicine and food homologous Radix Millettiae and application of the high-value cellobiose. The method comprises the following steps: extracting cellulose from Radix Millettiae; optimizing a beta-glucanase enzymolysis process by using a response surface method; after the enzymolysis liquid is subjected to selective removal of glucose through yeast fermentation, the high-value cellobiose product is obtained through acetone precipitation and freeze-drying. The process condition is mild and environment-friendly, high-value utilization of agricultural waste is realized, the obtained cellobiose product has high purity, and an economic and effective approach is provided for large-scale production of cellobiose. Animal experiments prove that the cellobiose product has a significant relieving effect on colonitis induced by dextran sulfate sodium in mice, can effectively improve disease symptoms, reduce oxidative stress, repair intestinal barrier and regulate inflammatory response, and has a good application prospect in preparation of functional food or medicine for relieving colonitis and high-value utilization of Radix Millettiae.

The invention provides UDP-glucose transferase for catalyzing glycosylation at C2'site of aescin, a gene, a primer group and application, and belongs to the technical field of aescin. The method is based on aesculus chinensis genome and transcriptome data analysis; according to the present invention, the UGT gene for catalyzing the C2'site of the protoaescinogenin-3-O-beta-D-glucopyranuronide is excavated, and the tobacco transient expression system is utilized to verify that the AcUGT94AK1, the AcUGT94AK3 and the AcUGT94AK6 can catalyze the protoaescinogenin-3-O-beta-D-glucopyranuronide to form the protoaescinogenin-3-O-beta-D-glucopyranuronide-(1-> 2)-beta-D-glucoside, such that the UGT gene can be used for catalyzing the C2 'site of the protoaescinogenin-3-O-beta-D-glucopyranuronide; the invention not only provides an important gene element for biosynthesis of aescin compounds, but also provides a key gene locus for molecular breeding of aesculus chinensis, and provides a theoretical basis for glycosylation modification of other triterpenoid saponins.

The invention discloses a liposome composition for rapidly improving facial edema and relaxation as well as a preparation method and application of the liposome composition, and belongs to the technical field of daily cosmetics. The liposome composition adopts a'core-shell 'double-layer structure design, a shell layer component comprises hydrogenated lecithin, beta-sitosterol, polyoxyethylene 40 hydrogenated castor oil, a spanishneedles herb extract and a kadsura longepedunculata extract, and a core layer component comprises acetyl hexapeptide-8 and an aesculus chinensis extract. According to the lipidosome composition, through the synergistic effect of multiple components and the slow release and transdermal enhancement effects of the core-shell lipidosome, the skin microcirculation is remarkably improved, the collagen generation is increased, the skin compactness is enhanced, and the problems of facial edema and relaxation are efficiently solved.

The invention discloses an aesculetin extraction method and application of aesculetin in plant disease control, and belongs to the technical field of plant disease control, the extraction method comprises the following steps: (1) raw material pretreatment: crushing and sieving a cortex fraxini raw material to obtain cortex fraxini powder; (2) extraction treatment: mixing the cortex fraxini powder with a methanol solution to obtain a mixed solution, then placing the mixed solution in an ultrasonic cleaner for extraction to obtain an extracting solution after the extraction is finished, transferring the extracting solution into a water bath heating reflux device, and after the reaction is finished, carrying out reduced pressure suction filtration while the extracting solution is hot to obtain aesculetin. And the application field is narrow. Meanwhile, the aesculetin is proved to have a broad-spectrum induced resistance effect and can effectively prevent and treat various diseases such as tobacco bacterial wilt, target leaf spot and virus diseases.

The invention provides a C24-site hydroxylase gene CYP7140E67 of stamen eugenin C, a CYP7140E67 protein and application of the C24-site hydroxylase gene CYP7140E67 and belongs to the technical field of genes. According to the invention, the key enzyme for catalyzing hydroxylation of the C24 site of the stamen eugenin C is successfully found and identified, the blank of research on the catalytic enzyme in aesculus chinensis is filled, and a key gene and a protein coding sequence are provided for biosynthetic pathway analysis and synthetic biology research of the original aescin and derivatives thereof. Moreover, the proaescin is heterogeneously synthesized by expressing the CYP714E67 in the Bensi tobacco leaves, a living body biosynthesis system is constructed, the CYP714E67 is heterogeneously expressed by utilizing saccharomyces cerevisiae, and the in-vitro synthesis of the proaescin is realized by virtue of yeast microsomes, so that a flexible path is provided for industrial production; the problem of resource supply of aescin A is solved, and a solid foundation is laid for promoting cultivation of new varieties of high-yield plants and industrial biosynthesis.