Composition for regulating skin mood as well as preparation method and application thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses a composition for regulating skin emotion and a preparation method and application thereof. Comprising the following components: aesculus chinensis fruit dry powder, radix sophorae flavescentis dry powder, mint leaf dry powder, geranium leaf dry powder and a solvent. The invention provides a composition for regulating skin mood, which is prepared by compounding dried aesculus chinensis fruit powder, dried radix sophorae flavescentis root powder, dried mint leaf powder and dried geranium leaf powder, and the prepared composition can regulate endogenous'happy hormone 'neurotransmitters (beta-enorphin, cortisol, 5-hydroxytryptamine, noradrenaline and the like) of a human body through skin absorption. Moreover, unhealthy states such as skin sensitivity, tension, redness and swelling, stabbing pain and pruritus caused by mood and pressure problems can be effectively relieved, expression of pressure regulating genes is activated, skin mood is relieved, skin detoxification is promoted, external pollution is defended, oxidation resistance and inflammation diminishing are achieved, and skin is relieved and repaired.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition for regulating skin mood, a preparation method and application thereof. Background Art

[0002] In modern society, the pace of life is accelerating, competition is intensifying, and pressure is increasing. When faced with difficulties, setbacks, and illnesses, anxiety and depression often make people lose sleep and appetite. How to regulate, control, and overcome negative emotions and maintain a positive attitude is a problem that each of us needs to face.

[0003] Human emotions, behaviors and hormone secretion are governed by the hypothalamus, which is connected to the cerebral cortex and the pituitary gland and other nerve tissues. It is the center for regulating nerves, body fluids and endocrine. Neurocosmetic researchers have found that the skin, as the largest sensory organ of the human body, has a large number of nerve endings distributed under its stratum corneum, which enables the skin to receive information from the external environment and transmit it to the nerve tissue. Therefore, from the perspective of neurocosmetology, emotional stress, nerve endings and skin can be correlated.

[0004] Emotions, as a resource, need to be replenished in time. From the perspective of organizational behavior, three ways to replenish emotional energy have been found: one is to use external physiological resources such as sleep and fitness; the second is to force the brain to jump out of the current situation such as meditation; and the third is to obtain positive feedback from intimate relationships. As an important part of life, beauty and skin care are one of the external physiological resources to replenish emotions. Consumers also have new demands for beauty products, and want to enhance the value of healthy emotions through makeup, skin care, and fragrance. In 2004, French skin neurobiologists proposed the concept of neurocosmetics, which is a new interdisciplinary subject that integrates neuroscience, psychology, dermatology, and cosmetic science. It is believed that when the active ingredients in cosmetics act on the nerve endings in the skin in a targeted manner, they can interfere with the transmission of nerve signals on the skin or the communication between skin nerves and other cells, and adjust and relieve the skin's sensitivity to cold, heat, tingling, itching, and pressure. Products that achieve emotional skin care by affecting the secretion of emotional hormones are called "neurocosmetics" that can communicate with the brain.

[0005] At present, Western medicine and Chinese patent medicine are mostly used to regulate negative emotions at home and abroad. Western medicine has large toxic side effects, and long-term use will damage the liver and kidneys. The effect of Chinese patent medicine is very slow, and most of them are taken by taking drugs or biological preparations, but there are not many external biological preparations on the market to improve skin mood sub-health, and most of the ingredients for nursing effects are unclear, the mechanism of action is unclear, and the effect is difficult to guarantee. Therefore, it is necessary to provide a composition for regulating skin emotions and its preparation method and application, so as to regulate skin emotions and relieve skin problems. Summary of the invention

[0006] The present invention aims to solve at least one of the technical problems existing in the above-mentioned prior art. To this end, the present invention proposes a composition for regulating skin emotions, a preparation method and an application thereof, which can effectively relieve unhealthy conditions such as skin sensitivity, tightness, redness, swelling, tingling, itching, etc. caused by emotions and stress problems, effectively regulate the human body's "happy hormone" neurotransmitters, activate the expression of stress regulating genes, relieve skin emotions, promote skin detoxification, defend against external pollution, resist oxidation and inflammation, and soothe and repair the skin.

[0007] A first aspect of the present invention provides a composition for regulating skin mood.

[0008] Specifically, the composition comprises the following components: horse chestnut dry powder, sophora flavescens root dry powder, mint leaf dry powder, geranium leaf dry powder and solvent.

[0009] Preferably, the following components are included by weight: 25-40 parts of horse chestnut dry powder, 1-8 parts of sophora flavescens root dry powder, 5-15 parts of peppermint leaf dry powder, 5-15 parts of geranium leaf dry powder, and 180-400 parts of solvent.

[0010] Preferably, the solvent includes at least one of ethanol, butanediol, propylene glycol and hexylene glycol.

[0011] More preferably, the solvent comprises ethanol and butanediol.

[0012] More preferably, the mass ratio of ethanol to butanediol is 1 to 3:3.

[0013] A second aspect of the present invention provides a method for preparing a composition for regulating skin mood.

[0014] Specifically, the following steps are included:

[0015] (1) adding horse chestnut fruit powder, Sophora flavescens root powder, peppermint leaf powder and geranium leaf powder to a solvent, stirring to obtain a mixed solution;

[0016] (2) setting the temperature, and then pumping the mixed solution from step (1) into the microfluidic extractor, adjusting the flow rate, stirring and extracting the mixed solution through the extractor, centrifuging, and collecting the separated solution;

[0017] (3) The separated liquid in step (2) is subjected to rotary evaporation, filtration, and decolorization to obtain a composition for regulating skin mood.

[0018] Preferably, in step (2), the set temperature is 40-50°C.

[0019] Further preferably, in step (2), the set temperature is 45-50°C.

[0020] More preferably, in step (2), the set temperature is 45°C.

[0021] Preferably, in step (2), the adjusted flow rate is 300 to 380 m / s.

[0022] Preferably, in step (2), the stirring extraction time is 15 to 20 hours.

[0023] Further preferably, in step (2), the stirring extraction time is 18 to 20 hours.

[0024] More preferably, in step (2), the stirring extraction time is 18 hours.

[0025] Preferably, in step (2), the centrifugal speed is 3500-4500 r / min, and the time is 80-100 min.

[0026] Further preferably, in step (2), the centrifugal speed is 4000-4500 r / min, and the time is 90-100 min.

[0027] More preferably, in step (2), the centrifugal speed is 4000 r / min and the time is 90 min.

[0028] A third aspect of the present invention provides a use of a composition for regulating skin mood in the preparation of cosmetics.

[0029] Preferably, the cosmetics include facial masks, essences, lotions, facial creams, skin care water, essence lotions, facial cleansers, makeup removers, freeze-dried agents, and isolation lotions.

[0030] A fourth aspect of the present invention provides an application method.

[0031] Specifically, the following steps are included:

[0032] The composition for regulating skin mood is added to the cosmetics in an amount of 0.1 to 15% by total weight.

[0033] Horse chestnut is a deciduous tree plant of the genus Aesculus in the Sapindaceae family. Its seeds, the seeds of the horse chestnut tree, are known as magical seeds and have been included in the Chinese Pharmacopoeia. Horse chestnut extracts mainly contain triterpenoid saponins, sugars, coumarins, brass and other active ingredients, which can protect the integrity of arterial blood vessels and structures, improve venous function, and have multiple effects such as anti-oxidation and anti-oxidation. It has anti-inflammatory, anti-inflammatory exudation, anti-edema, restores capillary permeability, increases venous tension, improves blood circulation, promotes cell recovery, resists oxygen free radicals, and has anti-tumor effects.

[0034] As a medicinal plant, Sophora flavescens has the effects of clearing away heat, reducing inflammation, antibacterial, diuretic, and removing dampness. It also has multiple effects such as antiviral, anti-tumor, and anti-allergic. Sophora flavescens root extract contains active molecules such as matrine, oxymatrine, hydroxymatrine, N-methyl cytisine, anagerine, indigoferine, dehydromatrine, d-isomatrine, and matrine. It has a good anti-inflammatory effect on common skin problems such as skin redness, inflammation, and has a good relief effect on skin itching. It can also reduce the release of allergic mediators in the body, thereby playing an immunosuppressive role. In addition, the alkaloids in Sophora flavescens root extract also have a significant inhibitory effect on bacterial respiration. It can balance oil secretion, dredge and astringe pores, remove toxins and impurities in the skin, promote the growth and repair of damaged vascular nerve cells, restore the vitality of subcutaneous capillary cells, and make the skin firmer, tenderer, and smoother.

[0035] Mint is one of the commonly used Chinese medicines. It is a pungent and cool diaphoretic and antipyretic drug, which can treat influenza, headache, red eyes, fever, sore throat, gum swelling and pain. It can be used externally to treat neuralgia, skin itching, rash and eczema. Mint leaves contain peppermint oil, menthol, menthone, isomenthone, rosmarinic acid and other ingredients. The unique volatile oil components in mint leaves, such as menthol and menthone, can quickly activate the cold receptors on the skin and bring an immediate cool feeling through nerve conduction. The unique cool and aromatic smell of mint leaves has a refreshing effect. The analgesic and anti-inflammatory effects of mint leaves come from its ability to stimulate nerve endings and promote blood circulation, thereby reducing local pain, relieving anxiety and stress, helping to relax the body and mind, and increasing the pleasure of mood. Regulating nerves Mint has the effect of stimulating the central nervous system. It has a burning and cold feeling on the skin. At the same time, it has an inhibitory and paralyzing effect on sensory nerve endings; therefore, it can be used as an anti-irritant and skin stimulant. It has anti-allergic and antipruritic effects on skin itching, and has obvious relief and analgesic effects on neuralgia and rheumatic joint pain. Mint leaves promote wound healing and reduce inflammation symptoms at the wound site. Mint extract has antioxidant effects, accelerates skin metabolism, and promotes wound healing.

[0036] As a traditional Chinese medicine, geranium is cold in nature and sweet in taste. It has the effects of clearing away heat and resolving phlegm, dispersing nodules and reducing swelling. It can be used for eczema, burns, sunburn, and relieving depression and anxiety. The various trace ingredients contained in geranium can calm the nerves and relieve stress, which can help patients relieve their emotions, calm the depression and anxiety of people who are often depressed, and because it can also affect the adrenal cortex, it can relieve stress and restore psychological balance. The stems and leaves of geranium contain volatile oils, which can be extracted from aromatic alcohol, vanillin, geranyl esters, etc., which have a good therapeutic effect on human fatigue, neurasthenia and other symptoms. When the skin is itchy, geranium can be used as a therapeutic drug for skin cleaning, which helps to relieve the symptoms of itching and has anti-inflammatory, bactericidal and itching effects. Geranium has a significant effect on the skin, can reduce dryness or peeling of the skin, and provide the skin tissue with the necessary nutrients. It can also prevent the formation of acne, spots and blackheads on the skin, and even repair damaged skin cells and increase skin elasticity.

[0037] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0038] The present invention provides a composition for regulating skin emotions. The composition is prepared by compounding horse chestnut dry powder, sophora flavescens root dry powder, mint leaf dry powder, and geranium leaf dry powder. The prepared composition can be absorbed through the skin and regulate endogenous "happy hormone" neurotransmitters (β-endorphin, cortisol, 5-hydroxytryptamine, norepinephrine, etc.) in the human body. In addition, it can effectively relieve unhealthy conditions such as skin sensitivity, tightness, redness, swelling, tingling, itching, etc. caused by emotions and stress problems, activate the expression of stress regulation genes, relieve skin emotions, promote skin detoxification, defend against external pollution, resist oxidation and inflammation, and soothe and repair the skin. DETAILED DESCRIPTION

[0039] In order to make the technical scheme of the present invention more clearly understood by those skilled in the art, the following embodiments are listed for illustration. It should be pointed out that the following embodiments do not limit the protection scope of the present invention.

[0040] Unless otherwise specified, the raw materials, reagents or devices used in the following examples can be obtained from conventional commercial sources or by existing known methods.

[0041] Preparation of horse chestnut fruit powder, sophora flavescens root powder, peppermint leaf powder, and geranium leaf powder:

[0042] 1. The horse chestnut fruit and the root of Sophora flavescens are selected, washed repeatedly and cut into 1-2 mm thin slices; the mint leaves and the geranium leaves are selected, washed repeatedly, dried and crushed;

[0043] 2. Add 5 times of pure water respectively, soak for 12 hours, extract at 98-100℃ for 6 hours, and filter for the first time to obtain the first extract;

[0044] 3. Add 3 times of pure water to the residue filtered for the first time, keep it at 98-100℃ for 6 hours, then extract and filter to obtain the second extract;

[0045] 4. Mix the first extract and the second extract, pump the mixture into the microfluidizer, and adjust the flow rate to 200-300 m / s. After all the materials pass through the extractor, stir and extract for 12 hours, centrifuge at 3000 r / min for 60 minutes to achieve rapid and strong collision of the materials, causing the plant cells to rupture instantly and collect the extract, and collect the separation liquid;

[0046] 5. Separate the separated liquid, obtain the supernatant and perform 0.45 μm fine filtration;

[0047] 6. Maintain the temperature at 75-78°C for concentration and control the density to 1.3-1.35 to obtain a concentrated solution;

[0048] 7. Dry the concentrated solution at a constant temperature of 75-78°C and control the moisture content below 5% to obtain horse chestnut fruit powder, sophora flavescens root powder, mint leaf powder and geranium leaf powder respectively;

[0049] 8. The horse chestnut dry powder, the sophora flavescens root dry powder, the mint leaf dry powder, and the geranium leaf dry powder were respectively pulverized and tested through a 65-mesh sieve to obtain the horse chestnut dry powder, the sophora flavescens root dry powder, the mint leaf dry powder, and the geranium leaf dry powder.

[0050] Example 1

[0051] A composition for regulating skin mood and a preparation method thereof.

[0052] The composition for regulating skin mood consists of 25 parts of horse chestnut dry powder, 2 parts of sophora flavescens root dry powder, 5 parts of peppermint leaf dry powder, 5 parts of geranium leaf dry powder and 185 parts of solvent (ethanol: butanediol is 2:3) by weight.

[0053] The specific preparation method is as follows:

[0054] (1) adding 25 parts of horse chestnut dry powder, 2 parts of Sophora flavescens root dry powder, 5 parts of peppermint leaf dry powder and 5 parts of geranium leaf dry powder to 185 parts of solvent (ethanol:butanediol ratio is 2:3), stirring to obtain a mixed solution;

[0055] (2) setting the temperature to 45° C., pumping the mixed solution from step (1) into a microfluidizer, adjusting the flow rate to 320 m / s, and allowing the mixed solution to pass through the extractor, stirring and extracting for 18 h, and centrifuging at 4000 r / min for 90 min to achieve rapid and strong collision of the materials, causing the plant cells to rupture instantly, collecting the extract, and obtaining a separated solution;

[0056] (3) The separated liquid in step (2) is subjected to rotary evaporation to reduce the total volume to 1 / 3, filtered through a 0.22 μm filter membrane, and the filtrate is added to a composite decolorizing resin for decolorization to obtain a composition for regulating skin mood.

[0057] Example 2

[0058] A composition for regulating skin mood and a preparation method thereof.

[0059] The composition for regulating skin mood consists of 30 parts of horse chestnut dry powder, 5 parts of sophora flavescens root dry powder, 10 parts of peppermint leaf dry powder, 10 parts of geranium leaf dry powder and 275 parts of solvent (ethanol:butanediol is 2:3) by weight.

[0060] The specific preparation method is as follows:

[0061] (1) adding 30 parts of horse chestnut dry powder, 5 parts of Sophora flavescens root dry powder, 10 parts of peppermint leaf dry powder and 10 parts of geranium leaf dry powder to 275 parts of solvent (ethanol:butanediol ratio is 2:3), stirring to obtain a mixed solution;

[0062] (2) setting the temperature to 45° C., pumping the mixed solution from step (1) into a microfluidizer, adjusting the flow rate to 350 m / s, and allowing the mixed solution to pass through the extractor, stirring and extracting for 18 h, and centrifuging at 4000 r / min for 90 min to achieve rapid and strong collision of the materials, causing the plant cells to rupture instantly, collecting the extract, and obtaining a separated solution;

[0063] (3) The separated liquid in step (2) is subjected to rotary evaporation to reduce the total volume to 1 / 3, filtered through a 0.22 μm filter membrane, and the filtrate is added to a composite decolorizing resin for decolorization to obtain a composition for regulating skin mood.

[0064] Example 3

[0065] A composition for regulating skin mood and a preparation method thereof.

[0066] The composition for regulating skin mood consists of 40 parts of horse chestnut dry powder, 8 parts of sophora flavescens root dry powder, 15 parts of mint leaf dry powder, 15 parts of geranium leaf dry powder and 390 parts of solvent (ethanol: butanediol is 2:3) by weight.

[0067] The specific preparation method is as follows:

[0068] (1) adding 40 parts of horse chestnut dry powder, 8 parts of Sophora flavescens root dry powder, 15 parts of peppermint leaf dry powder and 15 parts of geranium leaf dry powder to 390 parts of solvent (ethanol:butanediol ratio is 2:3), stirring to obtain a mixed solution;

[0069] (2) setting the temperature to 45° C., pumping the mixed solution from step (1) into a microfluidizer, adjusting the flow rate to 380 m / s, and allowing the mixed solution to pass through the extractor, stirring and extracting for 18 h, and centrifuging at 4000 r / min for 90 min to achieve rapid and strong collision of the materials, causing the plant cells to rupture instantly, collecting the extract, and obtaining a separated solution;

[0070] (3) The separated liquid in step (2) is subjected to rotary evaporation to reduce the total volume to 1 / 3, filtered through a 0.22 μm filter membrane, and the filtrate is added to a composite decolorizing resin for decolorization to obtain a composition for regulating skin mood.

[0071] Comparative Example 1

[0072] A composition for regulating skin mood and a preparation method thereof.

[0073] Compared with Example 3, no Sophora flavescens root dry powder was added in Comparative Example 1, and the remaining components and preparation method were the same as those in Example 3.

[0074] Comparative Example 2

[0075] A composition for regulating skin mood and a preparation method thereof.

[0076] Compared with Example 3, no mint leaf powder was added in Comparative Example 2, and the remaining components and preparation method were the same as those in Example 3.

[0077] Comparative Example 3

[0078] A composition for regulating skin mood and a preparation method thereof.

[0079] Compared with Example 3, no geranium leaf dry powder was added in Comparative Example 3, and the remaining components and preparation method were the same as those in Example 3.

[0080] Comparative Example 4

[0081] A composition for regulating skin mood and a preparation method thereof.

[0082] Compared with Example 3, in Comparative Example 4, no horse chestnut dry powder was added, and the remaining components and preparation method were the same as those in Example 3.

[0083] Comparative Example 5

[0084] A composition for regulating skin mood and a preparation method thereof.

[0085] Compared with Example 1, the amount of horse chestnut dry powder in Comparative Example 5 is 80 parts, and the remaining components and preparation method are the same as those in Example 1.

[0086] Comparative Example 6

[0087] A composition for regulating skin mood and a preparation method thereof.

[0088] Compared with Example 1, the amount of horse chestnut dry powder in Comparative Example 6 is 2 parts, and the other components and preparation method are the same as those in Example 1.

[0089] Performance testing:

[0090] 1. Appearance, aroma, pH, heat resistance and cold resistance tests.

[0091] According to the "Technical Specification for Safety of Cosmetics" (2015 edition), the compositions for regulating skin mood prepared in Examples 1 to 3 and Comparative Examples 1 to 6 were tested, as shown in Table 1.

[0092] Table 1 Appearance, odor, pH, heat resistance, cold resistance test results

[0093]

[0094] 2. Microbiological testing.

[0095] With reference to the microbial testing methods in Chapter 5 of the "Technical Specification for Safety of Cosmetics" (2015 edition), microbial testing was performed on the skin mood regulating compositions prepared in Examples 1-3 and Comparative Examples 1-6. The results are shown in Table 2.

[0096] Table 2 Microbiological test results

[0097]

[0098]

[0099] 3. Physical and chemical testing.

[0100] With reference to the physical and chemical testing methods in Chapter 4 of the "Technical Specification for Safety of Cosmetics" (2015 edition), microbial testing was performed on the skin mood regulating compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 6. The results are shown in Table 3.

[0101]

[0102] 4. Test for regulating the production of cortisol (hydrocortisone).

[0103] The specific steps are as follows:

[0104] Skin model: Reconstructed epidermis model (Episkin).

[0105] Culture conditions: 37.0°C, 5.0% CO2, saturated humidity.

[0106] Reagents: Episkin maintenance culture medium (special culture medium for skin model); Episkin detection culture medium: special culture medium for skin model.

[0107] sample:

[0108] Control group samples: diluted with purified water to 1% cortisone (hydrocortisone precursor);

[0109] Samples of Examples 1 to 3 and Comparative Examples 1 to 6 were diluted with purified water to a test concentration of 2%.

[0110] Skin model preparation: Add pre-warmed maintenance medium into a 12-well culture plate (2 mL well), transfer the skin model to the plate containing the maintenance medium, and place in an incubator for overnight culture.

[0111] Sample addition of Examples 1 to 3 and Comparative Examples 1 to 6: 1 μM of 1% cortisone (hydrocortisone precursor) was evenly applied to the surface of the epidermal model for 10 minutes, and then 10 μL of 2% samples of Examples 1 to 3 and Comparative Examples 1 to 6 were taken and incubated for 2 hours, the culture medium of each well model was collected, and the cortisol (hydrocortisone) content was determined by high performance liquid chromatography (HPLC).

[0112] Control group sampling: 1 μM 1% cortisone (hydrocortisone precursor) was evenly applied to the surface of the epidermal model for 10 minutes, and then 10 μL of purified water was taken for further incubation for 2 hours. The culture medium of each well model was collected, and the cortisol (hydrocortisone) content was determined by high performance liquid chromatography (HPLC).

[0113] Blank group sampling: 1 μM purified water was evenly applied to the surface of the epidermal model for 10 min, and then 10 μL purified water was taken to continue incubation for 2 h. The culture medium of each well model was collected, and the cortisol (hydrocortisone) content was determined by high performance liquid chromatography (HPLC).

[0114] The calculation formula of the test results is: inhibition rate = (control group-experimental group) / (control group-blank group)×100%. The results are shown in Table 4.

[0115] Table 4 Cortisol production test results

[0116] Cortisol (hydrocortisone) release (pg / mL) Inhibition rate (%) Example 1 57 64.3 Example 2 41 74.7 Example 3 29 82.5 Comparative Example 1 101 35.7 Comparative Example 2 106 32.5 Comparative Example 3 104 33.8 Comparative Example 4 110 29.9 Comparative Example 5 95 39.6 Comparative Example 6 132 15.6 Control group 156 / Blank group 2 /

[0117] As can be seen from Table 4, the effects of inhibiting the release of cortisol in Examples 1 to 3 are better than those in Comparative Examples 1 to 6, and the inhibitory effect of Example 3 is the best.

[0118] 5. Test to promote the production of β-endorphin (soothing neuropeptide).

[0119] The specific steps are as follows:

[0120] Skin model: Reconstructed epidermis model (Episkin).

[0121] Culture conditions: 37.0°C, 5.0% CO2, saturated humidity.

[0122] Reagents: Episkin maintenance culture medium (culture medium dedicated to skin model), Episkin detection culture medium (culture medium dedicated to skin model).

[0123] sample:

[0124] Concentration of control group samples: diluted with purified water to 1% dibutyryl AMPc;

[0125] Samples of Examples 1 to 3 and Comparative Examples 1 to 6 were diluted with purified water to a test concentration of 2%.

[0126] Skin model preparation: Add pre-warmed maintenance medium into a 12-well culture plate (2 mL well), transfer the skin model to the plate containing the maintenance medium, and place in an incubator for overnight culture.

[0127] Sample addition of Examples 1 to 3 and Comparative Examples 1 to 6: 1 μM 1% dibutyryl AMPc was evenly applied to the surface of the epidermal model for treatment for 30 minutes, and then 10 μL 2% samples of Examples 1 to 3 and Comparative Examples 1 to 6 were taken and continued to be incubated for 24 hours. The culture medium of each well model was collected, treated with ELISA reagent, and the β-endorphin content was determined by spectrophotometry.

[0128] Control group sampling: 1 μM 1% dibutyryl AMPc was evenly applied on the surface of the epidermal model for 30 minutes, and then 10 μL purified water was taken for further incubation for 24 hours. The culture medium of each well model was collected, treated with ELISA reagent, and the β-endorphin content was measured by spectrophotometry.

[0129] Blank group sampling: 1 μM purified water was evenly applied on the surface of the epidermal model for 30 min, and then 10 μL purified water was taken for further incubation for 24 h. The culture medium of each well model was collected and then treated with ELISA reagent, and the β-endorphin content was measured by spectrophotometry.

[0130] Calculation formula: increase rate = (experimental group - control group) / control group × 100%. The results are shown in Table 5.

[0131] Table 5 β-endorphin production test results

[0132]

[0133]

[0134] As can be seen from Table 5, the effects of promoting the release of β-endorphin in Examples 1 to 3 are better than those in Comparative Examples 1 to 6, and the promoting effect of Example 3 is the best.

[0135] 6. Test for activation of expression of stress resistance genes.

[0136] The specific steps are as follows:

[0137] Skin model: Reconstructed epidermis model (Episkin).

[0138] Culture conditions: 37.0°C, 5.0% CO2, saturated humidity.

[0139] Reagents: Episkin maintenance culture medium (culture medium dedicated to skin model), Episkin detection culture medium (culture medium dedicated to skin model).

[0140] sample:

[0141] Samples of Examples 1 to 3 and Comparative Examples 1 to 6 were diluted with purified water to a test concentration of 2%.

[0142] Skin model preparation: Add pre-warmed maintenance medium into a 12-well culture plate (2 mL well), transfer the skin model to the plate containing the maintenance medium, and place in an incubator for overnight culture.

[0143] Sample addition of Examples 1 to 3 and Comparative Examples 1 to 6: 10 μL of 2% samples of Examples 1 to 3 and Comparative Examples 1 to 6 were incubated for 48 h, the culture medium of each well model was collected, treated with ELISA reagent, and the β-endorphin content was determined by spectrophotometry.

[0144] Blank group sampling: 10 μL purified water was taken and incubated for 48 h. The culture medium of each well model was collected and treated with ELISA reagent. HMOX1, NQO1, IL-1A, ABCA12, and KLK5 were detected by spectrophotometry.

[0145] HMOX1: Heme oxygenase 1, a protective antioxidant enzyme that can decompose heme, release antioxidant metabolites carbon monoxide and biliverdin, and release free iron associated with oxidative stress and cardiac damage, playing an important role in regulating immune and inflammatory responses. It is often used to evaluate the effects of anti-cellular stress and antioxidant.

[0146] NQO1: An important antioxidant enzyme whose main function in cells is to convert NADH and NADPH into NAD + and NADP + , thus protecting cells from external quinones and oxidative damage. It is often used to evaluate the effects of antioxidants and detoxification.

[0147] IL-1A: Interleukin-1α (IL-1α) is a major alarm cytokine that triggers and enhances inflammatory responses. The structure and biological activity of IL-1α have been extensively studied and is considered an important regulator of inflammatory and hematopoietic responses. IL-1α is mainly produced by activated macrophages, neutrophils, epithelial cells, and endothelial cells, and is involved in the responses of the body's hematopoietic, nervous, and endocrine systems, as well as certain anti-tumor pathophysiological processes. It also has metabolic, physiological, and hematopoietic activities, and plays a core role in regulating immune responses. It is often used to evaluate anti-inflammatory and soothing effects.

[0148] ABCA12: It is a transmembrane transport protein, whose main function is to transport lipids, including ceramides. ABCA12 is responsible for transporting lipid proteins and accumulating them in the lamellae. When the cells on the surface of the skin die after keratinization, these accumulated lipids will be released and become important skin barriers and lipid layers, which play an important role in restoring the barrier function of damaged skin. It is often used to evaluate the effect of skin barrier repair.

[0149] KLK5: It is a serine protease that is mainly expressed in the epidermis of the skin. It is involved in the shedding of the stratum corneum and the regulation of the skin barrier function. The proteolytic activation cascade is initiated by the activation of KLK5 itself. KLK5 is a serine protease in CE (keratin mantle) and plays an important role in regulating the inflammatory response of the skin. They are epidermal products that regulate inflammation and ECM remodeling capabilities, so they can produce skin damage responses in the dermis during skin wound healing. They are often used to regulate skin inflammation and repair skin barrier effects.

[0150] The relative increase fold of each gene is the increase fold of each group of samples relative to the blank group. The results of each group are shown in Table 6.

[0151] Table 6 Expression results of each gene

[0152]

[0153]

[0154] It can be seen from the test results in the above table that Examples 1 to 3 can significantly promote the increase of HMOX1, NQO1, ABCA12, and KLK5, and can significantly inhibit the release of IL-1A. Among them, the promotion and inhibition effects of Example 3 are the best, proving that the composition for regulating skin emotions prepared from horse chestnut dry powder, sophora flavescens root dry powder, mint leaf dry powder and geranium leaf dry powder has a better synergistic effect, and within a certain range, the greater the ratio of the four dry powders, the better the effect. Based on the gene expression results, it can be seen that the composition of the present invention can activate the expression of anti-stress genes, that is, anti-cellular stress, anti-oxidation, detoxification, anti-inflammatory, soothing, and promoting skin barrier repair. The effects of Comparative Examples 1 to 6 are all worse than those of Examples 1 to 3.

[0155] Application Example 1

[0156] A serum.

[0157] Calculated by mass percentage, the essence consists of 5% of the composition for regulating skin mood provided in Example 2, 5% of butylene glycol, 0.2% of carbomer, 2% of betaine, 0.05% of sodium hyaluronate, 0.2% of arginine, 1% of hexylene glycol, 0.3% of p-hydroxyacetophenone, and the balance is water.

[0158] Preparation method: comprising stirring and dissolving the components to obtain the essence.

[0159] Comparative Example 1

[0160] A serum.

[0161] Compared with Application Example 1, Control Example 1 does not add the composition for regulating skin mood, and the remaining components and preparation method are the same as those of Application Example 1.

[0162] Test method: 64 subjects with high stress, irritability, anxiety, sensitive facial skin, tightness, redness, swelling, tingling, and poor skin barrier function were selected, ranging in age from 18 to 50 years old, and divided into two groups. Group A used the essence prepared in Application Example 1 every morning and evening for 1 month, and Group B used the essence prepared in Control Example 1 every morning and evening for 1 month, and the experience evaluation was statistically analyzed. The self-evaluation results of the subjects are shown in Table 7 below.

[0163] Table 7 Evaluation results of each group

[0164]

[0165]

[0166] From the self-evaluation results of the subjects using the essence product, it can be seen that after using the essence product with the composition for regulating skin emotions for 15 days, more than 78% of the subjects felt that the product could soothe emotions, relieve anxiety, relieve fatigue, relieve negative emotions and stress, and solve problems such as dry skin, redness, and sensitivity; after using it for 1 month, more than 90% of the subjects felt that the product could soothe emotions, relieve anxiety, relieve fatigue, relieve negative emotions and stress, and solve problems such as dry skin, redness, and sensitivity. This shows that the composition for regulating skin emotions in the present invention has the effects of soothing emotions, relieving anxiety, relieve fatigue, relieve negative emotions and stress, and solving problems such as dry skin, redness, and sensitivity.

[0167] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution obtained by any modification, equivalent replacement, improvement, etc. made by a person skilled in the art based on the concept of the present invention through logical analysis, reasoning or limited experiments on the basis of the prior art shall be within the scope of protection determined by the claims.

Claims

1. A composition for regulating skin mood, characterized in that: The invention comprises the following components: horse chestnut dry powder, sophora flavescens root dry powder, mint leaf dry powder, geranium leaf dry powder and solvent.

2. The composition according to claim 1, characterized in that The invention comprises the following components by weight: 25 to 40 parts of horse chestnut dry powder, 1 to 8 parts of sophora flavescens root dry powder, 5 to 15 parts of mint leaf dry powder, 5 to 15 parts of geranium leaf dry powder and 180 to 400 parts of solvent.

3. The composition according to claim 1, characterized in that The solvent includes at least one of ethanol, butanediol, propylene glycol and hexanediol.

4. A method for preparing the composition for regulating skin mood according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) adding horse chestnut fruit powder, Sophora flavescens root powder, peppermint leaf powder and geranium leaf powder to a solvent, stirring to obtain a mixed solution; (2) setting the temperature, and then pumping the mixed solution from step (1) into the microfluidic extractor, adjusting the flow rate, stirring and extracting the mixed solution through the extractor, centrifuging, and collecting the separated solution; (3) The separated liquid in step (2) is subjected to rotary evaporation, filtration, and decolorization to obtain a composition for regulating skin mood.

5. The preparation method according to claim 4, characterized in that: In step (2), the set temperature is 40-50°C.

6. The preparation method according to claim 4, characterized in that: In step (2), the flow rate is adjusted to 300-380 m / s.

7. The preparation method according to claim 4, characterized in that: In step (2), the stirring extraction time is 15 to 20 hours.

8. The preparation method according to claim 4, characterized in that: In step (2), the centrifugal speed is 3500-4500 r / min, and the time is 80-100 min.

9. Use of the composition for regulating skin mood according to any one of claims 1 to 3 in the preparation of cosmetics.

10. The use according to claim 9, characterized in that: The cosmetics include facial masks, essences, lotions, creams, skin care water, essence lotions, facial cleansers, makeup removers, freeze-dried agents, and isolation milks.

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