Gentiana macrophylla capsule fingerprint spectrum and application of spectrum to quality control and component analysis

A technology of fingerprint and capsule, applied in the field of drug analysis, to achieve the effect of good analysis and evaluation ability

Active Publication Date: 2018-03-13
XIAN C P PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the HPLC fingerprinting of Geranium Capsules

Method used

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  • Gentiana macrophylla capsule fingerprint spectrum and application of spectrum to quality control and component analysis
  • Gentiana macrophylla capsule fingerprint spectrum and application of spectrum to quality control and component analysis
  • Gentiana macrophylla capsule fingerprint spectrum and application of spectrum to quality control and component analysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The establishment of embodiment 1 fingerprint spectrum

[0041] 1 Instruments and reagents

[0042] Shimadzu high performance liquid chromatograph (LC-2010C HT , LC / Labsoluion chromatography workstation), Shimadzu Corporation, Japan; sartorius electronic balance, model CPA225D, Germany Sartorius company; ultrasonic generator, model KQ-5200DE CNC, Kunshan Ultrasonic Instrument Co., Ltd. Acetonitrile, methanol, chromatographic grade, Honeywell Company, USA; Ultrapure water, manufactured by Millipore Water Purifier, USA, Millipore Company, USA; loganic acid (lot number 111865-201403, content of 94.7%), methyl shanzhiside (lot number 111873-201103, with a content of 98.3%), stegopicroside (lot number 110785-201404, with a content of 98.3%), gentiopicroside (with a lot number of 110770-201716, with a content of 99.1%), isoorientin (lot number 111974) -201401, content of 94.0%) and other reference substances, all purchased from China Institute for the Control of Pharmaceuti...

Embodiment 2

[0052] Embodiment 2 Determination of each component content of Jilong capsule

[0053] 3.1 Chromatographic conditions The recording time is 60min, and the rest are the same as 2.1 of Example 1.

[0054] 3.2 The preparation of the reference substance solution Precisely weighed a certain amount of kaempferol methyl ester, picroside, picroside, isoorientin, isovitexin, loganin, 6′-O -β-D-glucosyl gentiopicroside, gentiopicroside, configured into a reference substance mixed solution, loganinic acid, kaempferol methyl ester, 6′-O-β-D- The concentrations of glucosyl gentiopicroside, steropicrin, gentiopicroside, erythromycin, isoorientin and isovitexin were 2100.00, 320.00, 1500.00, 510.00, 3900.00, 420.00, 220.00, 110μg·mL -1 .

[0055] 3.3 The preparation of the test solution is the same as 2.2.2 in Example 1.

[0056] 3.4 Linear relationship Precisely measure 0.01, 0.05, 0.10, 0.25, 0.50 and 1.00 mL of the reference substance mixture prepared in 3.2 and place them in each 1 m...

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Abstract

The invention relates to a gentiana macrophylla capsule fingerprint spectrum and an application of the spectrum to quality control and component analysis. According to the spectrum, a HPLC (high-performance liquid chromatography) fingerprint spectrum common mode of a gentiana macrophylla capsule is determined by the aid of a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system (2004A), 23 common peaks are calibrated, similarity is 0.966-0.982, and the content of loganic acid, shanzhiside methyl ester, 6'-O-beta-D-glucose gentiopicroside, swertiamarin,gentiopicroside, sweroside, isoorientin and isovitexin in 10 preparations is measured. According to the spectrum, a HPLC fingerprint spectrum method of the gentiana macrophylla capsule and a content measuring method are built, the method has good analysis evaluation ability and the advantages of accuracy, convenience, stability, reliability and the like, so that the method can serve as an effective evaluation method for the quality of the preparation.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to the fingerprint of Jilong capsules and its application in quality control and component analysis. Background technique [0002] The main component of Jilong Capsules is the extract of natural plant medicinal material Qinjie, which is mainly used for clearing the liver and releasing heat. [1] . Gentianamacrophylla (Gentianamacrophylla) is a plant of the Gentianaceae family. Its root is one of the important traditional Chinese medicinal materials in my country. It was first recorded in the "Shen Nong's Materia Medica". pee. Shaanxi and Gansu are the authentic producing areas of qinjie medicinal materials [2] . At present, the technical process of Jilong capsules produced by our company is as follows: Qinjing is permeated with 80% ethanol, then passed through a D101 type macroporous adsorption resin column, eluted with 30% ethanol, concentrated under reduced pressure of the eluent, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王四旺常瑛高茂煌肖会敏刘铁明袁鹰董明芝许志红
Owner XIAN C P PHARMA
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