UPLC method for detecting components in radix puerariae, radix puerariae extract and radix puerariae-containing preparation

Abstract

The present invention provides a UPLC method for qualitatively and quantitatively detecting the contents of six components such as 3'-hydroxy puerarin, puerarin, puerarin-6"-O-xyloside, 3'-methoxy puerarin, mirificin and daidzin in radix puerariae, radix puerariae extracts and radix puerariae-containing preparations by adopting puerarin as a reference substance. The UPLC method comprises: preparing a reference substance solution and a testing sample solution, and carrying out gradient elution by adopting 0.1% acetic acid as a mobile phase A and adopting acetonitrile as a mobile phase B, wherein the detection wavelength is 250 nm, the chromatographic column is SB-C18, RRHD 1.8 [mu]m, 2.1*100 mm, the column temperature is 31 DEG C, the flow rate is 0.4 mL / min, and the injection volume is 1 [mu]L; comparing the obtained chromatogram with a radix puerariae herb finger print, wherein the main peak is puerarin, and other components are corresponding to various peaks in a one-to-one manner so as to identify the herb; and calculating the contents of the six components in the radix puerariae by combining relative calibration factors (the relative calibration factor of 3'-hydroxy puerarin is 1.253, the relative calibration factor of puerarin is 1.000, the relative calibration factor of puerarin-6"-O-xyloside is 1.422, the relative calibration factor of 3'-methoxy puerarin is 1.297, the relative calibration factor of mirificin is 1.332, and the relative calibration factor of daidzin is 1.030) and the peak area of puerarin in the contrast solution.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to Chinese medicinal material extracts and extraction technology, and a UPLC rapid analysis method of preparations. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria lobata, which is distributed in most provinces of the country. Hubei is an important producing area. Deep processing of Pueraria lobata root into extract can produce better social and economic benefits. At the same time, the main component of Pueraria lobata extract is isoflavones, which has a wide range of pharmacological effects and prominent effects on cardiovascular and cerebrovascular diseases. The domestic and foreign markets have broad prospects. Pueraria lobata is used as the main medicine in many kinds of Chinese patent medicine prescriptions, and puerarin is selected as the quality control index. The components of traditional Chinese medicine are complex, and most of the time t...

AI Technical Summary

Problems solved by technology

However, due to limited conditions in the past, one of the easily detectable components (indicator components) was often selected to control the quality of Chinese medicinal materials, which would lead to some problems. On the one hand, the effect of medicinal materials may not be produced by this component. Therefore, it cannot fully reflect the quality of medicinal materials; on the other hand, in order to make huge profits, unscrupulous producers use counterfeit or inferior products to add ingredients that need to be detected by inspection standards. Therefore, multiple ingredients (indicative ingredients or Active ingredients) to establish corresponding quality control standards, so that artificial adulteration and adulteration can be well prevented, and industrial production can also be guided to ensure product quality

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