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Method for creating HPLC (high-performance liquid chromatography) fingerprint spectra of vinegar-processed rhizoma cyperi

A technology of fingerprints and construction methods, applied in the construction method of Cyperus cyperi HPLC fingerprints and the field of fingerprints, can solve problems such as lack, and achieve the effect of ensuring stability, reproducibility and good stability

Active Publication Date: 2018-08-21
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the lack of fingerprints in the prior art that can reflect the integrity and universality of the components contained in Cyperus cyperi, the present invention provides a method for constructing the HPLC fingerprints of Cyperia cyperi, which has strong operability

Method used

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  • Method for creating HPLC (high-performance liquid chromatography) fingerprint spectra of vinegar-processed rhizoma cyperi
  • Method for creating HPLC (high-performance liquid chromatography) fingerprint spectra of vinegar-processed rhizoma cyperi
  • Method for creating HPLC (high-performance liquid chromatography) fingerprint spectra of vinegar-processed rhizoma cyperi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Construction of the HPLC fingerprint of Cyperus cyperi

[0025] (1) Accurately weigh 0.5 g of Vinegar Cyperus decoction pieces powder, accurately add 20 mL of methanol, ultrasonically treat for 30 min, let it cool, make up for the lost weight with methanol, shake well, filter, and take the filtrate to obtain the test product solution;

[0026] (2) Accurately weigh the appropriate amount of reference substances of α-cyperone, cyperenone, and 5-hydroxymethylfurfural respectively, and add methanol to make different reference substance solutions; then precisely measure the appropriate amount of the above three reference substance solutions , placed in the same measuring bottle, diluted to the mark with methanol, and shaken evenly to obtain mixed reference substance solutions with concentrations of about 0.168mg / mL, 0.070mg / mL, and 0.069mg / mL respectively;

[0027] (3) Precisely draw 20 μL of the reference substance solution or the test solution and inject it into...

Embodiment 2

[0040] Example 2 Precision of Standard Fingerprint Construction Method

[0041] Take the same test solution in Example 1 (Sample No. 1), continuously inject 6 times, take No. 11 peak as the reference peak, calculate the relative retention time and relative peak area RSD value of No. 1-12 common fingerprint peaks (Table 3, Table 4) are all less than 3%, and the similarity of each chromatographic fingerprint calculated by the similarity evaluation software is greater than 0.90, indicating that the instrument method is stable and the precision is good.

[0042] Table 3 Relative peak area RSD of 6 consecutive injections of the same test product

[0043] .

[0044] Table 4 Relative retention time RSD of 6 consecutive injections of the same test product

[0045] .

Embodiment 3

[0046] Example 3 Repeatability of Standard Fingerprint Construction Method

[0047] Get the same batch of Vinegar Rhizoma Rhizoma Rhizoma Rhizoma Rhizoma Rhizome Powder (No. 1 sample) in Example 1, accurately weigh 6 parts, prepare 6 parts of test solution in parallel according to the method for preparing the test product in Example 1, inject samples respectively, and use No. 11 The peak is a reference peak, and the calculated relative retention time and relative peak area RSD values ​​(Table 5, Table 6) of No. 1-12 shared fingerprint peaks are both less than 3%, indicating that the construction method has good reproducibility.

[0048] Table 5 Relative peak area RSD of 6 consecutive injections of the same sample

[0049] .

[0050] Table 6 Relative retention time RSD of 6 consecutive injections of the same test product

[0051]

[0052] .

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Abstract

The invention provides a method for creating HPLC (high-performance liquid chromatography) fingerprint spectra of vinegar-processed rhizoma cyperi. The method includes extracting vinegar-processed rhizoma cyperi decoction piece powder by the aid of methanol to obtain test solution; utilizing alpha-cyperone, rhizoma cyperi ketene and 5-hydroxymethylfurfural as reference substances; measuring more than 10 batches of vinegar-processed rhizoma cyperi and carrying out analysis and comparison to obtain the HPLC fingerprint spectra of the vinegar-processed rhizoma cyperi. Each HPLC fingerprint spectrum comprises 12 common characteristic peaks. The method for creating the fingerprint spectra has the advantages that the method is excellent in precision, repeatability and stability; the quality of vinegar-processed rhizoma cyperi decoction pieces can be effectively integrally described and evaluated by the aid of the availability of common peaks in the fingerprint spectra, and accordingly the stability of the quality of the detection pieces and the effectiveness and the safety of clinical medication can be guaranteed.

Description

technical field [0001] The invention relates to the technical field of quality detection of traditional Chinese medicines, in particular to a method for constructing an HPLC fingerprint of Cyperus cyperi and its fingerprint. Background technique [0002] Cyperus cyperus Cyperus rotundus The dry rhizome of L. mainly contains volatile oil, flavonoids, alkaloids and triterpenoids. Cyperus vinegar enhances the effect of soothing the liver, regulating qi and relieving pain. Vinegar products are commonly used in Chinese medicine. There have been modern studies comparing the changes in the content of α-cyperone, cyperenone, narone, total flavonoids, luteolin, and trace elements before and after the production of Cyperus cyperi vinegar, but the evaluation indicators of these studies are single, or the test samples The amount is too small to characterize the difference of components before and after the production of Cyperus cyperi, and it is difficult to reflect the integrity and...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张超郭菲陈智张学兰李慧芬杨立梅
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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