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Establishment method of baphicacanthus cusia HPLC fingerprint

A technology of fingerprints and Nanbanlangen, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to fully reflect the overall information of chemical components, and achieve high quality control, good separation, and good reproducibility.

Active Publication Date: 2014-11-05
贵州中医药大学
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] Among the currently known technologies, the existing quality control methods of Nanbanlangen are mainly limited by the inability to fully reflect the overall information of the chemical composition

Method used

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  • Establishment method of baphicacanthus cusia HPLC fingerprint
  • Establishment method of baphicacanthus cusia HPLC fingerprint
  • Establishment method of baphicacanthus cusia HPLC fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: The establishment method of the HPLC fingerprint of Radix radix radix medicinal material

[0021] 1 Instruments, samples, reagents and reagents

[0022] 1.1 Instrument: Agilent 1100 high performance liquid chromatograph, DAD detector, Agilent Chemstation chromatography workstation, chromatographic column: Agilent ZorBax Eclipse XDB-C 18 (250mm×4.6mm, 5μm);

[0023] 1.2 Samples: 11 batches of samples of Nanlan root medicinal material, which came from different regions in Guizhou Province, and were identified as the Acanthaceae plant Malan Baphiicacant huscusia (Nees) Dry rhizomes and roots of Bremek;

[0024] 1.3 Reagent: Adenosine (batch number: 879-200001) was purchased from China Institute for the Control of Pharmaceutical and Biological Products;

[0025] 1.4 Reagents: Methanol is chromatographically pure, water is pure water, and other reagents are analytically pure;

[0026] 2 methods

[0027] 2.1 Preparation of the test solution: take about ...

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Abstract

The invention provides an establishment method of a baphicacanthus cusia HPLC fingerprint. The method includes following steps: preparing a to-be-tested sample solution, determining chromatographic conditions, and performing detection with an HPLC instrument to obtain a HPLC fingerprint of a baphicacanthus cusia medicine and extracts thereof. Overall chemical information of the baphicacanthus cusia can be comprehensively expressed and fingerprint characteristics of the baphicacanthus cusia can be determined. The fingerprint is simple in method, is good in repeatability, is many in characteristic peaks and is accurate and reliable. An established HPLC fingerprint can effectively controls quality of the baphicacanthus cusia medicine.

Description

technical field [0001] The invention relates to a method for establishing an HPLC (high-performance liquid chromatography) fingerprint of the Radix Isatidis medicinal material and an HPLC standard fingerprint of the Radix Radix Radix Radix medicinal material obtained thereby, belonging to the technical field of drug quality analysis. Background technique [0002] Nanbanlan root is derived from the acanthaceae plant Malan Baphica canthus cusia (Nees) The dried roots and rhizomes of Bremek. Isatis indigotica Roots of Fort.) are different in terms of base source, chemical composition, clinical application, etc. Nanbanlangen is one of the authentic medicinal materials in Guizhou Province. It has the effects of clearing heat and detoxifying, cooling blood and reducing swelling. Mainly cultivated. However, with the gradual expansion of its market, the demand for medicinal materials has increased, and now the artificial planting of Radix isatidis has become an important part o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86
Inventor 张丽艳杨丹黄健徐昌艳罗君谢宇卿娟
Owner 贵州中医药大学
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